RNF14 (NM_004290) Human Tagged ORF Clone

CAT#: RG223304

RNF14 (GFP-tagged) - Human ring finger protein 14 (RNF14), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RNF14
• Synonyms: ARA54; HFB30; HRIHFB2038; TRIAD2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223304 representing NM_004290
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTCAGAAGATCGAGAAGCTCAGGAGGATGAATTGCTGGCCCTGGCAAGTATTTACGATGGAGATG
  AATTTAGAAAAGCAGAGTCTGTCCAAGGTGGAGAAACCAGGATCTATTTGGATTTGCCACAGAATTTCAA
  GATATTTGTGAGCGGCAATTCAAATGAGTGTCTCCAGAATAGTGGCTTTGAATACACCATTTGCTTTCTG
  CCTCCACTTGTGCTGAACTTTGAACTGCCACCAGATTATCCATCCTCTTCCCCACCTTCATTCACACTTA
  GTGGCAAATGGCTGTCACCAACTCAGCTATCTGCTCTATGCAAGCACTTAGACAACCTATGGGAAGAACA
  CCGTGGCAGCGTGGTCCTGTTTGCCTGGATGCAATTTCTTAAGGAAGAGACCCTAGCATACTTGAATATT
  GTCTCTCCTTTTGAGCTCAAGATTGGTTCTCAGAAAAAAGTGCAGAGAAGGACAGCTCAAGCTTCTCCCA
  ACACAGAGCTAGATTTTGGAGGAGCTGCTGGATCTGATGTAGACCAAGAGGAAATTGTGGATGAGAGAGC
  AGTGCAGGATGTGGAATCACTGTCAAATCTGATCCAGGAAATCTTGGACTTTGATCAAGCTCAGCAGATA
  AAATGCTTTAATAGTAAATTGTTCCTGTGCAGTATCTGTTTCTGTGAGAAGCTGGGTAGTGAATGCATGT
  ACTTCTTGGAGTGCAGGCATGTGTACTGCAAAGCCTGTCTGAAGGACTACTTTGAAATCCAGATCAGAGA
  TGGCCAGGTTCAATGCCTCAACTGCCCAGAACCAAAGTGCCCTTCGGTGGCCACTCCTGGTCAGGTCAAA
  GAGTTAGTGGAAGCAGAGTTATTTGCCCGTTATGACCGCCTTCTCCTCCAGTCCTCCTTGGACCTGATGG
  CAGATGTGGTGTACTGCCCCCGGCCGTGCTGCCAGCTGCCTGTGATGCAGGAACCTGGCTGCACCATGGG
  TATCTGCTCCAGCTGCAATTTTGCCTTCTGTACTTTGTGCAGGTTGACCTACCATGGGGTCTCCCCATGT
  AAGGTGACTGCAGAGAAATTAATGGACTTACGAAATGAATACCTGCAAGCGGATGAGGCTAATAAAAGAC
  TTTTGGATCAAAGGTATGGTAAGAGAGTGATTCAGAAGGCACTGGAAGAGATGGAAAGTAAGGAGTGGCT
  AGAGAAGAACTCAAAGAGCTGCCCATGTTGTGGAACTCCCATAGAGAAATTAGACGGATGTAACAAGATG
  ACATGTACTGGCTGTATGCAATATTTCTGTTGGATTTGCATGGGTTCTCTCTCTAGAGCAAACCCTTACA
  AACATTTCAATGACCCTGGTTCACCATGTTTTAACCGGCTGTTTTATGCTGTGGATGTTGACGACGATAT
  TTGGGAAGATGAGGTAGAAGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223304 representing NM_004290
  Red=Cloning site Green=Tags(s)
  
  MSSEDREAQEDELLALASIYDGDEFRKAESVQGGETRIYLDLPQNFKIFVSGNSNECLQNSGFEYTICFL
  PPLVLNFELPPDYPSSSPPSFTLSGKWLSPTQLSALCKHLDNLWEEHRGSVVLFAWMQFLKEETLAYLNI
  VSPFELKIGSQKKVQRRTAQASPNTELDFGGAAGSDVDQEEIVDERAVQDVESLSNLIQEILDFDQAQQI
  KCFNSKLFLCSICFCEKLGSECMYFLECRHVYCKACLKDYFEIQIRDGQVQCLNCPEPKCPSVATPGQVK
  ELVEAELFARYDRLLLQSSLDLMADVVYCPRPCCQLPVMQEPGCTMGICSSCNFAFCTLCRLTYHGVSPC
  KVTAEKLMDLRNEYLQADEANKRLLDQRYGKRVIQKALEEMESKEWLEKNSKSCPCCGTPIEKLDGCNKM
  TCTGCMQYFCWICMGSLSRANPYKHFNDPGSPCFNRLFYAVDVDDDIWEDEVED
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004290
• ORF Size: 1422 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_004290.4, NP_004281.1
• RefSeq Size: 4395
• RefSeq ORF: 1425
• Locus ID: 9604
• Domains: RING, IBR, RWD
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Six alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq, Jan 2011]