TMEM158 (NM_015444) Human Tagged ORF Clone

CAT#: RG224658

TMEM158 (GFP-tagged) - Human transmembrane protein 158 (gene/pseudogene) (TMEM158)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TMEM158
• Synonyms: BBP; p40BBP; RIS1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224658 representing NM_015444
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCCCTGCTCGCCGCGCTCCTGGCCGCCGCCTGCCCGCTGCCGCCCGTCCGCGGCGGGGCCGCGG
  ACGCGCCCGGCCTCCTCGGGGTGCCCTCCAATGCTTCAGTCAACGCGTCCTCCGCGGACGAGCCCATCGC
  CCCGCGGCTGCTGGCCTCGGCGGCCCCCGGGCCCCCCGAGCGCCCGGGCCCGGAGGAGACGGCGGCGGCA
  GCGGCGCCGTGCAACATCAGCGTGCAGCGGCAGATGCTGAGCTCGCTGCTCGTGCGCTGGGGCCGCCCGC
  GGGGCTTCCAGTGCGACCTACTGCTCTTCTCCACCAACGCGCACGGCCGCGCTTTCTTCGCCGCCGCCTT
  CCACCGCGTCGGGCCGCCGCTGCTCATCGAGCACCTGGGGCTGGCGGCGGGCGGCGCGCAGCAGGACCTG
  CGCCTCTGCGTGGGCTGCGGCTGGGTGCGCGGTCGCCGCACTGGCCGCCTCCGGCCCGCCGCCGCCCCCA
  GCGCCGCCGCCGCCACCGCCGGGGCGCCCACCGCGCTGCCAGCCTACCCCGCGGCCGAGCCGCCCGGGCC
  GCTGTGGCTGCAGGGCGAGCCGCTGCATTTCTGCTGCCTAGACTTCAGCCTGGAGGAGCTGCAGGGCGAG
  CCGGGCTGGCGGCTGAACCGTAAGCCCATTGAGTCCACGCTGGTGGCCTGCTTCATGACCCTGGTCATCG
  TGGTGTGGAGCGTGGCCGCCCTCATCTGGCCGGTGCCCATCATCGCCGGCTTCCTGCCCAACGGCATGGA
  ACAGCGCCGGACCACCGCCAGCACCACCGCAGCCACCCCCGCCGCAGTGCCCGCAGGGACCACCGCAGCC
  GCCGCCGCCGCCGCCGCTGCCGCCGCCGCCGCGGCCGTCACTTCGGGGGTGGCGACCAAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224658 representing NM_015444
  Red=Cloning site Green=Tags(s)
  
  MLPLLAALLAAACPLPPVRGGAADAPGLLGVPSNASVNASSADEPIAPRLLASAAPGPPERPGPEETAAA
  AAPCNISVQRQMLSSLLVRWGRPRGFQCDLLLFSTNAHGRAFFAAAFHRVGPPLLIEHLGLAAGGAQQDL
  RLCVGCGWVRGRRTGRLRPAAAPSAAAATAGAPTALPAYPAAEPPGPLWLQGEPLHFCCLDFSLEELQGE
  PGWRLNRKPIESTLVACFMTLVIVVWSVAALIWPVPIIAGFLPNGMEQRRTTASTTAATPAAVPAGTTAA
  AAAAAAAAAAAAVTSGVATK
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015444
• ORF Size: 900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015444.1, NP_056259.1
• RefSeq Size: 1797
• RefSeq ORF: 903
• Locus ID: 25907
• Protein Families: Druggable Genome
• Gene Summary: Constitutive activation of the Ras pathway triggers an irreversible proliferation arrest reminiscent of replicative senescence. Transcription of this gene is upregulated in response to activation of the Ras pathway, but not under other conditions that induce senescence. The encoded protein is similar to a rat cell surface receptor proposed to function in a neuronal survival pathway. An allelic polymorphism in this gene results in both functional and non-functional (frameshifted) alleles; the reference genome represents the functional allele. [provided by RefSeq, Jul 2015]