TMEM158 (NM_015444) Human Tagged ORF Clone

CAT#: RC224658

TMEM158 (Myc-DDK-tagged)-Human transmembrane protein 158 (gene/pseudogene) (TMEM158)

Product Images

Western blot validation of overexpression lysate (Cat# LY414544) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224658 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TMEM158
• Synonyms: BBP; p40BBP; RIS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224658 representing NM_015444.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCTGCCCCTGCTCGCCGCGCTCCTGGCCGCCGCCTGCCCGCTGCCGCCCGTCCGCGGCGGGGCCGCG
  GACGCGCCCGGCCTCCTCGGGGTGCCCTCCAATGCTTCAGTCAACGCGTCCTCCGCGGACGAGCCCATC
  GCCCCGCGGCTGCTGGCCTCGGCGGCCCCCGGGCCCCCCGAGCGCCCGGGCCCGGAGGAGACGGCGGCG
  GCAGCGGCGCCGTGCAACATCAGCGTGCAGCGGCAGATGCTGAGCTCGCTGCTCGTGCGCTGGGGCCGC
  CCGCGGGGCTTCCAGTGCGACCTACTGCTCTTCTCCACCAACGCGCACGGCCGCGCTTTCTTCGCCGCC
  GCCTTCCACCGCGTCGGGCCGCCGCTGCTCATCGAGCACCTGGGGCTGGCGGCGGGCGGCGCGCAGCAG
  GACCTGCGCCTCTGCGTGGGCTGCGGCTGGGTGCGCGGTCGCCGCACTGGCCGCCTCCGGCCCGCCGCC
  GCCCCCAGCGCCGCCGCCGCCACCGCCGGGGCGCCCACCGCGCTGCCAGCCTACCCCGCGGCCGAGCCG
  CCCGGGCCGCTGTGGCTGCAGGGCGAGCCGCTGCATTTCTGCTGCCTAGACTTCAGCCTGGAGGAGCTG
  CAGGGCGAGCCGGGCTGGCGGCTGAACCGTAAGCCCATTGAGTCCACGCTGGTGGCCTGCTTCATGACC
  CTGGTCATCGTGGTGTGGAGCGTGGCCGCCCTCATCTGGCCGGTGCCCATCATCGCCGGCTTCCTGCCC
  AACGGCATGGAACAGCGCCGGACCACCGCCAGCACCACCGCAGCCACCCCCGCCGCAGTGCCCGCAGGG
  ACCACCGCAGCCGCCGCCGCCGCCGCCGCTGCCGCCGCCGCCGCGGCCGTCACTTCGGGGGTGGCGACC
  AAG
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by RC224658
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MLPLLAALLAAACPLPPVRGGAADAPGLLGVPSNASVNASSADEPIAPRLLASAAPGPPERPGPEETAA
  AAAPCNISVQRQMLSSLLVRWGRPRGFQCDLLLFSTNAHGRAFFAAAFHRVGPPLLIEHLGLAAGGAQQ
  DLRLCVGCGWVRGRRTGRLRPAAAPSAAAATAGAPTALPAYPAAEPPGPLWLQGEPLHFCCLDFSLEEL
  QGEPGWRLNRKPIESTLVACFMTLVIVVWSVAALIWPVPIIAGFLPNGMEQRRTTASTTAATPAAVPAG
  TTAAAAAAAAAAAAAAVTSGVATK
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015444
• ORF Size: 900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015444.1, NP_056259.1
• RefSeq Size: 1797 bp
• RefSeq ORF: 903 bp
• Locus ID: 25907
• UniProt ID: Q8WZ71
• Cytogenetics: 3p21.31
• Protein Families: Druggable Genome
• MW: 30.4 kDa
• Gene Summary: Constitutive activation of the Ras pathway triggers an irreversible proliferation arrest reminiscent of replicative senescence. Transcription of this gene is upregulated in response to activation of the Ras pathway, but not under other conditions that induce senescence. The encoded protein is similar to a rat cell surface receptor proposed to function in a neuronal survival pathway. An allelic polymorphism in this gene results in both functional and non-functional (frameshifted) alleles; the reference genome represents the functional allele. [provided by RefSeq, Jul 2015]