Topoisomerase II beta (TOP2B) (NM_001068) Human Tagged ORF Clone

CAT#: RG224802

TOP2B (GFP-tagged) - Human topoisomerase (DNA) II beta 180kDa (TOP2B)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TOP2B
• Synonyms: top2beta; TOPIIB
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224802 representing NM_001068
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAGTCGGGTGGCTGCGGCGCGGGAGCCGGCGTGGGCGGCGGCAACGGGGCACTGACCTGGGTGA
  ACAATGCTGCAAAAAAAGAAGAGTCAGAAACTGCCAACAAAAATGATTCTTCAAAGAAGTTGTCTGTTGA
  GAGAGTGTATCAGAAGAAGACACAACTTGAACACATTCTTCTTCGTCCTGATACATATATTGGGTCAGTG
  GAGCCATTGACGCAGTTCATGTGGGTGTATGATGAAGATGTAGGAATGAATTGCAGGGAGGTTACCTTTG
  TGCCAGGTTTATACAAGATCTTTGATGAAATTTTGGTTAATGCTGCTGACAATAAACAGAGGGATAAGAA
  CATGACTTGTATTAAAGTTTCTATTGATCCTGAATCTAACATTATAAGCATTTGGAATAATGGGAAAGGC
  ATTCCAGTAGTAGAACACAAGGTAGAGAAAGTTTATGTTCCTGCTTTAATTTTTGGACAGCTTTTAACAT
  CCAGTAACTATGATGATGATGAGAAAAAAGTTACAGGTGGTCGTAATGGTTATGGTGCAAAACTTTGTAA
  TATTTTCAGTACAAAGTTTACAGTAGAAACAGCTTGCAAAGAATACAAACACAGTTTTAAGCAGACATGG
  ATGAATAATATGATGAAGACTTCTGAAGCCAAAATTAAACATTTTGATGGTGAAGATTACACATGCATAA
  CATTCCAACCAGATCTGTCCAAATTTAAGATGGAAAAACTTGACAAGGATATTGTGGCCCTCATGACTAG
  AAGGGCATATGATTTGGCTGGTTCGTGTAGAGGGGTCAAGGTCATGTTTAATGGAAAGAAATTGCCTGTA
  AATGGATTTCGCAGTTATGTAGATCTTTATGTGAAAGACAAATTGGATGAAACTGGGGTGGCCCTGAAAG
  TTATTCATGAGCTTGCAAATGAAAGATGGGATGTTTGTCTCACATTGAGTGAAAAAGGATTCCAGCAAAT
  CAGCTTTGTAAATAGTATTGCAACTACAAAAGGTGGACGGCACGTGGATTATGTGGTAGATCAAGTTGTT
  GGTAAACTGATTGAAGTAGTTAAGAAAAAGAACAAAGCTGGTGTATCAGTGAAACCATTTCAAGTAAAAA
  ACCATATATGGGTTTTTATTAATTGCCTTATTGAAAATCCAACTTTTGATTCTCAGACTAAGGAAAACAT
  GACTCTGCAGCCCAAAAGTTTTGGGTCTAAATGCCAGCTGTCAGAAAAATTTTTTAAAGCAGCCTCTAAT
  TGTGGCATTGTAGAAAGTATCCTGAACTGGGTGAAATTTAAGGCTCAGACTCAGCTGAATAAGAAGTGTT
  CATCAGTAAAATACAGTAAAATCAAAGGTATTCCCAAACTGGATGATGCTAATGATGCTGGTGGTAAACA
  TTCCCTGGAGTGTACACTGATATTAACAGAGGGAGACTCTGCCAAATCACTGGCTGTGTCTGGATTAGGT
  GTGATTGGACGAGACAGATACGGAGTTTTTCCACTCAGGGGCAAAATTCTTAATGTACGGGAAGCTTCTC
  ATAAACAGATCATGGAAAATGCTGAAATAAATAATATTATTAAAATAGTTGGTCTACAATATAAGAAAAG
  TTACGATGATGCAGAATCTCTGAAAACCTTACGCTATGGAAAGATTATGATTATGACCGATCAGGATCAA
  GATGGTTCTCACATAAAAGGCCTGCTTATTAATTTCATCCATCACAATTGGCCATCACTTTTGAAGCATG
  GTTTTCTTGAAGAGTTCATTACTCCTATTGTAAAGGCAAGCAAAAATAAGCAGGAACTTTCCTTCTACAG
  TATTCCTGAATTTGACGAATGGAAAAAACATATAGAAAACCAGAAAGCCTGGAAAATAAAGTACTATAAA
  GGATTGGGTACTAGTACAGCTAAAGAAGCAAAGGAATATTTTGCTGATATGGAAAGGCATCGCATCTTGT
  TTAGATATGCTGGTCCTGAAGATGATGCTGCCATTACCTTGGCATTTAGTAAGAAGAAGATTGATGACAG
  AAAAGAATGGTTAACAAATTTTATGGAAGACCGGAGACAGCGTAGGCTACATGGCTTACCAGAGCAATTT
  TTATATGGTACTGCAACAAAGCATTTGACTTATAATGATTTCATCAACAAGGAATTGATTCTCTTCTCAA
  ACTCAGACAATGAAAGATCTATACCATCTCTTGTTGATGGCTTTAAACCTGGCCAGCGGAAAGTTTTATT
  TACCTGTTTCAAGAGGAATGATAAACGTGAAGTAAAAGTTGCCCAGTTGGCTGGCTCTGTTGCTGAGATG
  TCGGCTTATCATCATGGAGAACAAGCATTGATGATGACTATTGTGAATTTGGCTCAGAACTTTGTGGGAA
  GTAACAACATTAACTTGCTTCAGCCTATTGGTCAGTTTGGAACTCGGCTTCATGGTGGCAAAGATGCTGC
  AAGCCCTCGTTATATTTTCACAATGTTAAGCACTTTAGCAAGGCTACTTTTTCCTGCTGTGGATGACAAC
  CTCCTTAAGTTCCTTTATGATGATAATCAACGTGTAGAGCCTGAGTGGTATATTCCTATAATTCCCATGG
  TTTTAATAAATGGTGCTGAGGGCATTGGTACTGGATGGGCTTGTAAACTACCCAACTATGATGCTAGGGA
  AATTGTGAACAATGTCAGACGAATGCTAGATGGCCTGGATCCTCATCCCATGCTTCCAAACTACAAAAAC
  TTTAAAGGCACGATTCAAGAACTTGGTCAAAACCAGTATGCAGTCAGTGGTGAAATATTTGTAGTGGACA
  GAAACACAGTAGAAATTACAGAGCTTCCAGTTAGAACTTGGACACAGGTATATAAAGAACAGGTTTTAGA
  ACCTATGCTAAATGGAACAGATAAAACACCAGCATTAATTTCTGATTATAAAGAATATCATACTGACACA
  ACTGTGAAATTTGTGGTGAAAATGACTGAAGAGAAACTAGCACAAGCAGAAGCTGCTGGACTGCATAAAG
  TTTTTAAACTTCAAACTACTCTTACTTGTAATTCCATGGTACTTTTTGATCATATGGGATGTCTGAAGAA
  ATATGAAACTGTGCAAGACATTCTGAAAGAATTCTTTGATTTACGATTAAGTTATTACGGTTTACGTAAG
  GAGTGGCTTGTGGGAATGTTGGGAGCAGAATCTACAAAGCTTAACAATCAAGCCCGTTTCATTTTAGAGA
  AGATACAAGGGAAAATTACTATAGAGAATAGGTCAAAGAAAGATTTGATTCAAATGTTAGTCCAGAGAGG
  TTATGAATCTGACCCAGTGAAAGCCTGGAAAGAAGCACAAGAAAAGGCAGCAGAAGAGGATGAAACACAA
  AACCAGCATGATGATAGTTCCTCCGATTCAGGAACTCCTTCAGGCCCAGATTTTAATTATATTTTAAATA
  TGTCTCTGTGGTCTCTTACTAAAGAAAAAGTTGAAGAACTGATTAAACAGAGAGATGCAAAAGGGCGAGA
  GGTCAATGATCTTAAAAGAAAATCTCCTTCAGATCTTTGGAAAGAGGATTTAGCGGCATTTGTTGAAGAA
  CTGGATAAAGTGGAATCTCAAGAACGAGAAGATGTTCTGGCTGGAATGTCTGGAAAAGCAATTAAAGGTA
  AAGTTGGCAAACCTAAGGTGAAGAAACTCCAGTTGGAAGAGACAATGCCCTCACCTTATGGCAGAAGAAT
  AATTCCTGAAATTACAGCTATGAAGGCAGATGCCAGCAAAAAGTTGCTGAAGAAGAAGAAGGGTGATCTT
  GATACTGCAGCAGTAAAAGTGGAATTTGATGAAGAATTCAGTGGAGCACCAGTAGAAGGTGCAGGAGAAG
  AGGCATTGACTCCATCAGTTCCTATAAATAAAGGTCCCAAACCTAAGAGGGAGAAGAAGGAGCCTGGTAC
  CAGAGTGAGAAAAACACCTACATCATCTGGTAAACCTAGTGCAAAGAAAGTGAAGAAACGGAATCCTTGG
  TCAGATGATGAATCCAAGTCAGAAAGTGATTTGGAAGAAACAGAACCTGTGGTTATTCCAAGAGATTCTT
  TGCTTAGGAGAGCAGCAGCCGAAAGACCTAAATACACATTTGATTTCTCAGAAGAAGAGGATGATGATGC
  TGATGATGATGATGATGACAATAATGATTTAGAGGAATTGAAAGTTAAAGCATCTCCCATAACAAATGAT
  GGGGAAGATGAATTTGTTCCTTCAGATGGGTTAGATAAAGATGAATATACATTTTCACCAGGCAAATCAA
  AAGCCACTCCAGAAAAATCTTTGCATGACAAAAAAAGTCAGGATTTTGGAAATCTCTTCTCATTTCCTTC
  ATATTCTCAGAAGTCAGAAGATGATTCAGCTAAATTTGACAGTAATGAAGAAGATTCTGCTTCTGTTTTT
  TCACCATCATTTGGTCTGAAACAGACAGATAAAGTTCCAAGTAAAACGGTAGCTGCTAAAAAGGGAAAAC
  CGTCTTCAGATACAGTCCCTAAGCCCAAGAGAGCCCCAAAACAGAAGAAAGTAGTAGAGGCTGTAAACTC
  TGACTCGGATTCAGAATTTGGCATTCCAAAGAAGACTACAACACCAAAAGGTAAAGGCCGAGGGGCAAAG
  AAAAGGAAAGCATCTGGCTCTGAAAATGAAGGCGATTATAACCCTGGCAGGAAAACATCCAAAACAACAA
  GCAAGAAACCGAAGAAGACATCTTTTGATCAGGATTCAGATGTGGACATCTTCCCCTCAGACTTCCCTAC
  TGAGCCACCTTCTCTGCCACGAACCGGTCGGGCTAGGAAAGAAGTAAAATATTTTGCAGAGTCTGATGAA
  GAAGAAGATGATGTTGATTTTGCAATGTTTAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224802 representing NM_001068
  Red=Cloning site Green=Tags(s)
  
  MAKSGGCGAGAGVGGGNGALTWVNNAAKKEESETANKNDSSKKLSVERVYQKKTQLEHILLRPDTYIGSV
  EPLTQFMWVYDEDVGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKG
  IPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTW
  MNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGKKLPV
  NGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVV
  GKLIEVVKKKNKAGVSVKPFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASN
  CGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLG
  VIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQ
  DGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYK
  GLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQF
  LYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEM
  SAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDN
  LLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKN
  FKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDT
  TVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRK
  EWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEDETQ
  NQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDAKGREVNDLKRKSPSDLWKEDLAAFVEE
  LDKVESQEREDVLAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIIPEITAMKADASKKLLKKKKGDL
  DTAAVKVEFDEEFSGAPVEGAGEEALTPSVPINKGPKPKREKKEPGTRVRKTPTSSGKPSAKKVKKRNPW
  SDDESKSESDLEETEPVVIPRDSLLRRAAAERPKYTFDFSEEEDDDADDDDDDNNDLEELKVKASPITND
  GEDEFVPSDGLDKDEYTFSPGKSKATPEKSLHDKKSQDFGNLFSFPSYSQKSEDDSAKFDSNEEDSASVF
  SPSFGLKQTDKVPSKTVAAKKGKPSSDTVPKPKRAPKQKKVVEAVNSDSDSEFGIPKKTTTPKGKGRGAK
  KRKASGSENEGDYNPGRKTSKTTSKKPKKTSFDQDSDVDIFPSDFPTEPPSLPRTGRARKEVKYFAESDE
  EEDDVDFAMFN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001068
• ORF Size: 4863 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001068.2, NP_001059.2
• RefSeq Size: 5360 bp
• RefSeq ORF: 4866 bp
• Locus ID: 7155
• Cytogenetics: 3p24.2
• Domains: DNA_gyraseB, DNA_topoisoIV, HATPase_c
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]'