PABPC4 (NM_001135653) Human Tagged ORF Clone

CAT#: RG226744

PABPC4 (GFP-tagged) - Human poly(A) binding protein, cytoplasmic 4 (inducible form) (PABPC4), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PABPC4
• Synonyms: APP-1; APP1; iPABP; PABP4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226744 representing NM_001135653
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGCTGCGGCCAGCAGCTACCCCATGGCCTCCCTGTACGTGGGCGACCTGCATTCGGACGTCACCG
  AGGCCATGCTGTACGAAAAGTTCAGCCCCGCGGGGCCTGTGCTGTCCATCCGGGTCTGCCGCGATATGAT
  CACCCGCCGCTCCCTGGGCTATGCCTACGTCAACTTCCAGCAGCCGGCCGACGCTGAGCGGGCTTTGGAC
  ACCATGAACTTTGATGTGATTAAGGGAAAGCCAATCCGCATCATGTGGTCTCAGAGGGATCCCTCTTTGA
  GAAAATCTGGTGTGGGAAACGTCTTCATCAAGAACCTGGACAAATCTATAGATAACAAGGCACTTTATGA
  TACTTTTTCTGCTTTTGGAAACATACTGTCCTGCAAGGTGGTGTGTGATGAGAACGGCTCTAAGGGTTAT
  GCCTTTGTCCACTTCGAGACCCAAGAGGCTGCCGACAAGGCCATCGAGAAGATGAATGGCATGCTCCTCA
  ATGACCGCAAAGTATTTGTGGGCAGATTCAAGTCTCGCAAAGAGCGGGAAGCTGAGCTTGGAGCCAAAGC
  CAAGGAATTCACCAATGTTTATATCAAAAACTTTGGGGAAGAGGTGGATGATGAGAGTCTGAAAGAGCTA
  TTCAGTCAGTTTGGTAAGACCCTAAGTGTCAAGGTGATGAGAGATCCCAATGGGAAATCCAAAGGCTTTG
  GCTTTGTGAGTTACGAAAAACACGAGGATGCCAATAAGGCTGTGGAAGAGATGAATGGAAAAGAAATAAG
  TGGTAAAATCATATTTGTAGGCCGTGCACAAAAGAAAGTAGAACGGCAGGCAGAGTTAAAACGGAAATTT
  GAACAGTTGAAACAGGAGAGAATTAGTCGATATCAGGGGGTGAATCTCTACATTAAGAACTTGGATGACA
  CTATTGATGATGAGAAATTAAGGAAAGAATTTTCTCCTTTTGGATCAATTACCAGTGCTAAGGTAATGCT
  GGAGGATGGAAGAAGCAAAGGGTTTGGCTTCGTCTGCTTCTCATCTCCTGAAGAAGCAACCAAAGCAGTC
  ACTGAGATGAATGGACGCATTGTGGGCTCCAAGCCACTATATGTTGCCCTGGCCCAGAGGAAGGAAGAGA
  GAAAGGCTCACCTGACCAACCAGTATATGCAACGAGTGGCTGGAATGAGAGCACTTCCTGCCAATGCCAT
  CTTAAATCAGTTCCAGCCTGCAGCGGGTGGCTACTTTGTGCCAGCAGTCCCACAGGCTCAGGGAAGGCCT
  CCATATTATACACCTAACCAGTTAGCACAGATGAGGCCTAATCCACGCTGGCAGCAAGGTGGGAGACCTC
  AAGGCTTCCAAGGAATGCCAAGTGCTATACGCCAGTCTGGGCCTCGTCCAACTCTTCGCCATCTGGCTCC
  AACTGGTAATGCTCCGGCCTCTCGTGGCCTCCCTACTACCACTCAGAGAGTCGGGTCTGAGTGCCCGGAC
  CGCTTGGCTATGGACTTTGGTGGGGCTGGTGCCGCCCAGCAAGGGCTGACTGACAGCTGCCAGTCTGGAG
  GCGTTCCCACAGCTGTGCAGAACTTAGCGCCACGCGCTGCTGTTGCTGCTGCTGCTCCCCGGGCTGTTGC
  CCCCTACAAATACGCCTCCAGTGTCCGCGGCCCTCATCCTGCCATACAGCCTCTGCAGGCACCCCAGCCT
  GCGGTCCATGTGCAGGGGCAGGAGCCACTGACTGCCTCCATGCTGGCTGCAGCACCCCCCCAGGAACAGA
  AGCAGATGCTGGGAGAACGCTTGTTCCCACTCATCCAAACAATGCATTCAAATCTGGCTGGGAAGATCAC
  GGGAATGCTGCTGGAGATAGACAACTCTGAGCTGCTGCACATGTTAGAGTCCCCCGAGTCTCTCCGCTCC
  AAGGTGGATGAAGCTGTAGCAGTTCTACAGGCTCATCATGCCAAGAAAGAAGCTGCCCAGAAGGTGGGCG
  CTGTTGCTGCTGCTACCTCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226744 representing NM_001135653
  Red=Cloning site Green=Tags(s)
  
  MNAAASSYPMASLYVGDLHSDVTEAMLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFQQPADAERALD
  TMNFDVIKGKPIRIMWSQRDPSLRKSGVGNVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGY
  AFVHFETQEAADKAIEKMNGMLLNDRKVFVGRFKSRKEREAELGAKAKEFTNVYIKNFGEEVDDESLKEL
  FSQFGKTLSVKVMRDPNGKSKGFGFVSYEKHEDANKAVEEMNGKEISGKIIFVGRAQKKVERQAELKRKF
  EQLKQERISRYQGVNLYIKNLDDTIDDEKLRKEFSPFGSITSAKVMLEDGRSKGFGFVCFSSPEEATKAV
  TEMNGRIVGSKPLYVALAQRKEERKAHLTNQYMQRVAGMRALPANAILNQFQPAAGGYFVPAVPQAQGRP
  PYYTPNQLAQMRPNPRWQQGGRPQGFQGMPSAIRQSGPRPTLRHLAPTGNAPASRGLPTTTQRVGSECPD
  RLAMDFGGAGAAQQGLTDSCQSGGVPTAVQNLAPRAAVAAAAPRAVAPYKYASSVRGPHPAIQPLQAPQP
  AVHVQGQEPLTASMLAAAPPQEQKQMLGERLFPLIQTMHSNLAGKITGMLLEIDNSELLHMLESPESLRS
  KVDEAVAVLQAHHAKKEAAQKVGAVAAATS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001135653
• ORF Size: 1980 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001135653.1, NP_001129125.1
• RefSeq Size: 3230
• RefSeq ORF: 1983
• Locus ID: 8761
• Gene Summary: Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]