DACH1 (NM_004392) Human Tagged ORF Clone

CAT#: RG229258

DACH1 (GFP-tagged) - Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 3. Note: ORF is codon optimized

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DACH1
• Synonyms: DACH
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG229258 ORF sequence, codon optimized.
  Due to the complexity of NM_004392, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGTGCCGGCAGCCCTGATCCCCCCAACACAGTTGGTCCCTCCTCAGCCTCCAATTTCCACAAGCG
  CCAGTTCTTCTGGTACGACTACTAGCACCAGTTCTGCTACATCTAGCCCTGCACCTTCTATTGGTCCGCC
  GGCGAGTTCTGGCCCGACGCTGTTTAGGCCAGAACCAATCGCTTCAGCCGCCGCCGCAGCCGCGACGGTT
  ACCTCCACAGGGGGAGGTGGCGGAGGTGGCGGTGGCGGCAGCGGCGGAGGCGGCGGAAGTAGCGGCAACG
  GGGGTGGAGGAGGAGGCGGTGGCGGAGGCTCTAACTGTAATCCAAACCTGGCAGCTGCGAGTAACGGCTC
  TGGGGGCGGTGGGGGCGGCATTTCTGCCGGCGGCGGCGTGGCTAGCTCTACACCCATTAATGCTAGTACA
  GGCTCCAGTTCAAGCTCAAGCAGTAGCTCTTCTAGTTCCTCATCTTCCAGCTCATCAAGCAGCTCTTCAT
  CTTCATGTGGTCCACTCCCAGGTAAACCAGTGTACAGCACCCCATCTCCCGTCGAGAATACGCCCCAAAA
  CAATGAGTGCAAGATGGTGGATCTGAGAGGAGCAAAAGTAGCGTCCTTCACAGTCGAAGGGTGTGAGCTG
  ATATGTCTCCCTCAGGCCTTTGACCTCTTCCTGAAACACCTGGTTGGCGGGCTGCACACTGTCTATACCA
  AGCTTAAACGCCTCGAAATCACACCGGTTGTGTGCAACGTCGAACAGGTGAGGATCCTCAGAGGTCTGGG
  GGCAATTCAGCCCGGCGTGAATAGGTGTAAACTCATCTCCCGCAAGGACTTTGAAACACTCTATAACGAC
  TGTACCAACGCTTCCAGCCGGCCAGGCCGGCCACCCAAAAGGACCCAGAGCGTCACAAGCCCAGAAAACT
  CACACATTATGCCCCACTCCGTCCCAGGACTGATGAGTCCTGGAATCATACCCCCGACAGACGAGACACC
  CCTCAGTACCCCTACAGCCCGCGACAGTCTCGACAAGCTGTCACTTACTGGACACGGACAACCCCTCCCA
  CCTGGGTTCCCAAGCCCATTTTTGTTCCCCGACGGCCTCAGTAGTATCGAGACGTTGCTGACTAACATTC
  AGGGCCTGCTGAAAGTTGCTATTGATAATGCCCGGGCCCAGGAGAAACAAGTTCAGCTCGAGAAAACAGA
  GTTGAAAATGGACTTTCTGCGGGAGCGCGAGTTGAGAGAGACGTTGGAAAAGCAGCTGGCTATGGAGCAG
  AAGAATCGCGCAATTGTGCAGAAACGGCTGAAAAAGGAAAAAAAGGCCAAGCGCAAGCTCCAGGAGGCAC
  TTGAATTTGAAACCAAGAGGAGAGAACAGGCGGAACAGACCCTCAAGCAGGCGGCAAGTACAGACTCCCT
  CAGGGTGTTGAATGATTCTCTCACGCCTGAGATTGAAGCTGACCGCAGCGGCGGTAGAACCGACGCTGAG
  CGGACCATCCAGGATGGACGACTCTATCTGAAAACTACAGTGATGTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG229258 representing NM_004392
  Red=Cloning site Green=Tags(s)
  
  MAVPAALIPPTQLVPPQPPISTSASSSGTTTSTSSATSSPAPSIGPPASSGPTLFRPEPIASAAAAAATV
  TSTGGGGGGGGGGSGGGGGSSGNGGGGGGGGGGSNCNPNLAAASNGSGGGGGGISAGGGVASSTPINAST
  GSSSSSSSSSSSSSSSSSSSSSSSSCGPLPGKPVYSTPSPVENTPQNNECKMVDLRGAKVASFTVEGCEL
  ICLPQAFDLFLKHLVGGLHTVYTKLKRLEITPVVCNVEQVRILRGLGAIQPGVNRCKLISRKDFETLYND
  CTNASSRPGRPPKRTQSVTSPENSHIMPHSVPGLMSPGIIPPTDETPLSTPTARDSLDKLSLTGHGQPLP
  PGFPSPFLFPDGLSSIETLLTNIQGLLKVAIDNARAQEKQVQLEKTELKMDFLRERELRETLEKQLAMEQ
  KNRAIVQKRLKKEKKAKRKLQEALEFETKRREQAEQTLKQAASTDSLRVLNDSLTPEIEADRSGGRTDAE
  RTIQDGRLYLKTTVMY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004392
• ORF Size: 1518 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004392.5, NP_004383.3
• RefSeq Size: 4634 bp
• RefSeq ORF: 1515 bp
• Locus ID: 1602
• Cytogenetics: 13q21.33
• Domains: Ski_Sno
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a chromatin-associated protein that associates with other DNA-binding transcription factors to regulate gene expression and cell fate determination during development. The protein contains a Ski domain that is highly conserved from Drosophila to human. Expression of this gene is lost in some forms of metastatic cancer, and is correlated with poor prognosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]'