DACH1 (NM_004392) Human Untagged Clone

CAT#: SC327893

DACH1 (untagged)-Human dachshund homolog 1 (Drosophila) (DACH1) transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DACH1
• Synonyms: DACH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004392, the custom clone sequence may differ by one or more nucleotides
  ATGGCAGTGCCGGCGGCTTTGATCCCTCCGACCCAGCTGGTCCCCCCTCAACCCCCAATC
  TCCACGTCTGCTTCCTCCTCTGGCACCACCACCTCCACCTCTTCGGCGACTTCGTCTCCG
  GCTCCTTCCATCGGACCCCCGGCGTCCTCTGGGCCAACTCTGTTCCGCCCGGAGCCCATC
  GCTTCGGCGGCGGCGGCGGCGGCCACAGTCACCTCTACCGGCGGCGGCGGCGGCGGCGGC
  GGCGGCGGCAGCGGAGGCGGCGGCGGCAGCAGCGGCAACGGAGGCGGCGGTGGCGGCGGC
  GGCGGTGGCAGCAACTGCAACCCCAACCTGGCGGCCGCGAGCAACGGCAGCGGCGGCGGC
  GGCGGCGGCATCAGCGCTGGCGGCGGCGTCGCTTCCAGCACCCCCATCAACGCCAGCACC
  GGCAGCAGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGCAGCAGTAGTAGCAGCAGCAGC
  AGTAGCAGCAGCAGCTGCGGCCCCCTCCCCGGGAAACCCGTGTACTCAACCCCGTCCCCA
  GTGGAAAACACCCCTCAGAATAATGAGTGCAAAATGGTGGATCTGAGGGGGGCCAAAGTG
  GCTTCCTTCACGGTGGAGGGCTGCGAGCTGATCTGCCTGCCCCAGGCTTTCGACCTGTTC
  CTGAAGCACTTGGTGGGGGGCTTGCATACGGTCTACACCAAGCTGAAGCGGCTGGAGATC
  ACGCCGGTGGTGTGCAATGTGGAACAAGTTCGCATCCTGAGGGGACTGGGCGCCATCCAG
  CCAGGAGTGAACCGCTGCAAACTCATCTCCAGGAAGGACTTCGAGACCCTCTACAATGAC
  TGCACCAACGCAAGTTCTAGACCTGGAAGGCCTCCTAAGAGGACTCAAAGTGTCACCTCC
  CCAGAGAACTCTCACATCATGCCGCATTCTGTCCCTGGTCTCATGTCTCCTGGGATAATT
  CCACCAACAGATGAGACCCCGCTTTCTACACCAACCGCAAGAGACAGCCTTGACAAACTC
  TCTCTAACTGGGCATGGACAACCACTGCCTCCAGGTTTTCCATCTCCTTTTCTGTTTCCT
  GATGGACTGTCTTCCATCGAGACTCTTCTGACTAACATACAGGGGCTGTTGAAAGTTGCC
  ATAGATAATGCCAGAGCTCAAGAGAAACAGGTCCAACTGGAAAAAACTGAGCTGAAGATG
  GATTTTTTAAGGGAAAGAGAACTAAGGGAAACACTTGAGAAGCAGTTGGCTATGGAACAA
  AAGAATAGAGCCATAGTTCAAAAGAGGCTAAAGAAGGAGAAGAAGGCAAAGAGAAAATTG
  CAGGAAGCACTTGAGTTTGAGACGAAACGGCGTGAACAAGCAGAACAGACGCTAAAACAG
  GCAGCTTCAACAGATAGTCTCAGGGTCTTAAATGACTCTCTGACCCCAGAGATAGAGGCT
  GACCGCAGTGGCGGCAGAACAGATGCTGAAAGGACAATACAAGATGGAAGACTGTATTTG
  AAAACTACTGTCATGTAC
  
• Restriction Sites: Please inquire
• ACCN: NM_004392
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004392.5, NP_004383.3
• RefSeq Size: 4640 bp
• RefSeq ORF: 1521 bp
• Locus ID: 1602
• Cytogenetics: 13q21.33
• Domains: Ski_Sno
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a chromatin-associated protein that associates with other DNA-binding transcription factors to regulate gene expression and cell fate determination during development. The protein contains a Ski domain that is highly conserved from Drosophila to human. Expression of this gene is lost in some forms of metastatic cancer, and is correlated with poor prognosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]'
  Transcript Variant: This variant (3) lacks four consecutive coding exons but maintains the same reading frame, compared to variant 1. The resulting isoform (c) is shorter than isoform a. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence because a full-length transcript was not available for this variant. The genomic coordinates used for the transcript record were based on RT-PCR data reported in PMID:11543628.