Cd8b (NM_031539) Rat Untagged Clone

CAT#: RN214135

Cd8b (untagged ORF) - Rat CD8b molecule (Cd8b), (10 ug)

Product Images

Circular map for RN214135

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Cd8b
• Synonyms: Cd8b1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >NM_031539 ORF sequence, RN214135 may differ due to SNPs.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCAGCCATGGCTCTGGCTGGTCTTCAGTGTGAAGCTATCAGCTCTCTGGGGCAGCTCTGCCCTCCTT
  CAGACTCCTTCATCCCTGCTGGTTCAAACCAACCAAACAGCAAAAATGTCCTGTGAGGCTAAAACCTTC
  CCTAAAGGAACAACCATCTACTGGCTGAGAGAGCTCCAGGATTCCAACAAGAACAAGCACTTTGAGTTC
  CTGGCGTCCCGGACTTCAACCAAAGGAATCAAATATGGTGAAAGGGTGAAGAAGAATATGACTCTGTCT
  TTCAATTCAACCCTGCCCTTTCTCAAGATCATGGATGTGAAACCAGAGGACAGTGGCTTCTACTTCTGC
  GCGATGGTTGGGAGTCCCATGGTAGTCTTTGGGACAGGGACAAAGCTGACTGTGGTTGATGTCCTTCCT
  ACAACTGCTCCAACCAAAAAGACTACACTGAAGAAGAAACAATGTCCAACCCCCCACCCAAAGACCCAG
  AAAGGCTTGACATGTGGCCTCATTACCCTCAGCCTGCTGGTGGCCTGCATCCTGGTTCTGCTGGTATCC
  CTCAGTGTGGCCATCCACTTTCACTGCACGCGGAGGAGAGCCCGGATTCGCTTCATGAAACAGTTTCAC
  AAATGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  MQPWLWLVFSVKLSALWGSSALLQTPSSLLVQTNQTAKMSCEAKTFPKGTTIYWLRELQDSNKNKHFEF
  LASRTSTKGIKYGERVKKNMTLSFNSTLPFLKIMDVKPEDSGFYFCAMVGSPMVVFGTGTKLTVVDVLP
  TTAPTKKTTLKKKQCPTPHPKTQKGLTCGLITLSLLVACILVLLVSLSVAIHFHCTRRRARIRFMKQFH
  K*
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
  Fully Sequenced ORF

  >RN214135 representing NM_031539
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCCATGGCTCTGGCTGGTCTTCAGTGTGAAGCTATCAGCTCTCTGGGGCAGCTCTGCCCTCCTTC
  AGACTCCTTCATCCCTGCTGGTTCAAACCAACCAAACAGCAAAAATGTCCTGTGAGGCTAAAACCTTCCC
  TAAAGGAACAACCATCTACTGGCTGAGAGAGCTCCAGGATTCCAACAAGAACAAGCACTTTGAGTTCCTG
  GCGTCCCGGACTTCAACCAAAGGAATTAAATATGGTGAAAGGGTGAAGAAGAATATGACTCTGTCTTTCA
  ATTCAACCCTGCCCTTTCTCAAGATCATGGATGTGAAGCCAGAGGACAGTGGCTTCTACTTCTGCGCGAT
  GGTTGGGAGTCCCATGGTAGTCTTTGGGACAGGGACAAAGCTGACTGTGGTTGATGTCCTTCCTACAACT
  GCTCCAACCAAAAAGACTACACTGAAGAAGAAACAATGTCCAACCCCCCACCCAAAGACCCAGAAAGGCT
  TGACATGTGGCCTCATTACCCTCAGCCTGCTGGTGGCCTGCATCCTGGTTCTGCTGGTATCCCTCAGTGT
  GGCCATCCACTTTCACTGCATGCGGAGGAGAGCCCGGATTCACTTCATGAAACAGTTTCACAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI Plasmid Map
• ACCN: NM_031539
• Insert Size: 627 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031539.2
• RefSeq Size: 1258 bp
• RefSeq ORF: 627 bp
• Locus ID: 24931
• UniProt ID: P05541
• Cytogenetics: 4q31
• MW: 23.4 kDa
• Gene Summary: a marker for cytotoxic T cells; involved in cell-cell interactions [RGD, Feb 2006]