EBF1 (NM_024007) Human Untagged Clone

CAT#: SC107271

EBF1 (untagged)-Human early B-cell factor 1 (EBF1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EBF1
• Synonyms: COE1; EBF; O/E-1; OLF1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_024007, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTTGGGATTCAGGAAAGCATCCAACGGAGTGGAAGCAGCATGAAGGAAGAGCCGCTGGGCAGCGGCA
  TGAACGCGGTGCGGACGTGGATGCAGGGCGCCGGGGTGCTGGACGCCAACACGGCGGCGCAGAGCGGGGT
  GGGTCTGGCCCGGGCTCACTTTGAGAAGCAGCCGCCTTCCAATCTGCGGAAATCCAACTTCTTCCACTTC
  GTCCTGGCCCTCTACGACAGACAGGGCCAGCCCGTGGAGATCGAGAGGACAGCGTTTGTGGGGTTCGTGG
  AGAAGGAAAAAGAAGCCAACAGCGAAAAGACCAATAACGGAATTCACTACCGGCTTCAGCTTCTCTACAG
  CAATGGGATAAGGACGGAGCAGGATTTCTACGTGCGCCTCATTGACTCCATGACAAAACAAGCCATAGTG
  TATGAAGGCCAAGACAAGAACCCAGAAATGTGCCGAGTCTTGCTCACACATGAGATCATGTGCAGCCGCT
  GTTGTGACAAGAAAAGCTGTGGCAACCGAAATGAGACTCCCTCAGATCCAGTGATAATTGACAGGTTCTT
  CTTGAAATTTTTCCTCAAATGTAACCAAAATTGCCTAAAGAATGCGGGAAACCCACGTGACATGCGGAGA
  TTCCAGGTCGTGGTGTCTACGACAGTCAATGTGGATGGCCATGTCCTGGCAGTCTCTGATAACATGTTTG
  TCCATAATAATTCCAAGCATGGGCGGAGGGCTCGGAGGCTTGACCCCTCGGAAGGTACGCCCTCTTATCT
  GGAACATGCTACTCCCTGTATCAAAGCCATCAGCCCGAGTGAAGGATGGACGACGGGAGGTGCGACTGTG
  ATCATCATAGGGGACAATTTCTTTGATGGGTTACAGGTCATATTCGGTACCATGCTGGTCTGGAGTGAGT
  TGATCACTCCTCATGCCATCCGTGTGCAGACCCCTCCTCGGCACATCCCTGGTGTTGTGGAAGTCACACT
  GTCCTACAAATCTAAGCAGTTCTGCAAAGGAACACCAGGCAGATTCATTTATACAGCGCTCAACGAACCC
  ACCATCGATTATGGTTTCCAGAGGTTACAGAAGGTCATTCCTCGGCACCCTGGTGACCCTGAGCGTTTGC
  CAAAGGAAGTAATACTCAAAAGGGCTGCGGATCTGGTAGAAGCACTGTATGGGATGCCACACAACAACCA
  GGAAATCATTCTGAAGAGAGCGGCCGACATTGCCGAGGCCCTGTACAGTGTTCCCCGCAACCACAACCAA
  CTCCCGGCCCTTGCTAACACCTCGGTCCACGCAGGGATGATGGGCGTGAATTCGTTCAGTGGACAACTGG
  CCGTGAATGTCTCCGAGGCATCACAAGCCACCAATCAGGGTTTCACCCGCAACTCAAGCAGCGTATCACC
  ACACGGGTACGTGCCGAGCACCACTCCCCAGCAGACCAACTATAACTCCGTCACCACGAGCATGAACGGA
  TACGGCTCTGCCGCAATGTCCAATTTGGGCGGCTCCCCCACCTTCCTCAACGGCTCAGCTGCCAACTCCC
  CCTATGCCATAGTGCCATCCAGCCCCACCATGGCCTCCTCCACAAGCCTCCCCTCCAACTGCAGCAGCTC
  CTCGGGCATCTTCTCCTTCTCACCAGCCAACATGGTCTCAGCCGTGAAACAGAAGAGTGCTTTCGCACCA
  GTCGTCAGACCCCAGACCTCCCCACCTCCCACCTGCACCAGCACCAACGGGAACAGCCTGCAAGCGATAT
  CTGGCATGATTGTTCCTCCTATGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024007 unedited
  NATACGACTCACTATAGGGGCGGCCGCGATTCGGCACGAGGGTTGTTTTCATGTTTGGGA
  TTCAGGAAAGCATCCAACGGAGTGGAAGCAGCATGAAGGAAGAGCCGCTGGGCAGCGGCA
  TGAACGCGGTGCGGACGTGGATGCAGGGCGCCGGGGTGCTGGACGCCAACACGGCGGCGC
  AGAGCGGGGTGGGTCTGGCCCGGGCTCACTTTGAGAAGCAGCCGCCTTCCAATCTGCGGA
  AATCCAACTTCTTCCACTTCGTCCTGGCCCTCTACGACAGACAGGGCCAGCCCGTGGAGA
  TCGAGAGGACAGCGTTTGTGGGGTTCGTGGAGAAGGAAAAAGAAGCCAACAGCGAAAAGA
  CCAATAACGGAATTCACTACCGGCTTCAGCTTCTCTACAGCAATGGGATAAGGACGGAGC
  AGGATTTCTACGTGCGCCTCATTGACTCCATGACAAAACAAGCCATAGTGTATGAAGGCC
  AAGACAAGAACCCAGAAATGTGCCGAGTCTTGCTCACACATGAGATCATGTGCAGCCGCT
  GTTGTGACAAGAAAAGCTGTGGCAACCGAAATGAGACTCCCTCAGATCCAGTGATAATTG
  ACAGGTTCTTCTTGAAATTTTTCCTCAAATGTAACCAAAATTGCCTAAAGAATGCGGGAA
  ANCCCACGTGACATGCGGAGATTCCNAGGTCGTGGTGTCTACGACAGTCATGGTGGATGG
  CCATGTCCCTGGCAGTCTCTGATAACAATGTTTGTCCTTAATAATTTCCAGCATGGNCGG
  GAGGGCTCGGGAGCCTTTGACCCCTCGGAAGCTACTCCCTGTATCAAAGCATCANCCCGA
  GTGAGGATGGACACCGGGAGGTGCAATTGTGATATCATAGGGGGACATTCTTGATGGGNT
  AAGGTCATATTCGACCCTGCTGGNCTGGGGAGAGATGGATACTCCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_024007
• Insert Size: 4400 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024007.2, NP_076870.1
• RefSeq Size: 2762 bp
• RefSeq ORF: 1776 bp
• Locus ID: 1879
• Cytogenetics: 5q33.3
• Protein Families: Transcription Factors
• Gene Summary: ''
  Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is one aa shorter compared to isoform 1.