EBF1 (NM_024007) Human Untagged Clone

CAT#: SC322605

EBF1 (untagged)-Human early B-cell factor 1 (EBF1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EBF1
• Synonyms: COE1; EBF; O/E-1; OLF1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322605 CCACGCGTCCGGCCAAGCATACATCTCTAATTTTATTATTTTGGTCTTTTCGTTGGATTT
  TCCCTTTCTTCTTTTTTTCGGGTTATCGCTCAGTTTTGAGCAGAGGTTTACATTTTTTAA
  AAATTTGCTTTCCAGCCCGCCTTGATCTTCTAAGTGCGAGTTCATCGTCTGAGAAAAAAA
  AAAATCTCTGGTTGGCGTTTTTGTTTCTTTTCTTTTCTTTCTTTTCTTTCCTTTTTTTTT
  TTTTTTTAATTTTTTTCAAGGGGGAGGAGATTTTCCACAAGAAAAGGTTGTTTTCATGTT
  TGGGATTCAGGAAAGCATCCAACGGAGTGGAAGCAGCATGAAGGAAGAGCCGCTGGGCAG
  CGGCATGAACGCGGTGCGGACGTGGATGCAGGGCGCCGGGGTGCTGGACGCCAACACGGC
  GGCGCAGAGCGGGGTGGGTCTGGCCCGGGCTCACTTTGAGAAGCAGCCGCCTTCCAATCT
  GCGGAAATCCAACTTCTTCCACTTCGTCCTGGCCCTCTACGACAGACAGGGCCAGCCCGT
  GGAGATCGAGAGGACAGCGTTTGTGGGGTTCGTGGAGAAGGAAAAAGAAGCCAACAGCGA
  AAAGACCAATAACGGAATTCACTACCGGCTTCAGCTTCTCTACAGCAATGGGATAAGGAC
  GGAGCAGGATTTCTACGTGCGCCTCATTGACTCCATGACAAAACAAGCCATAGTGTATGA
  AGGCCAAGACAAGAACCCAGAAATGTGCCGAGTCTTGCTCACACATGAGATCATGTGCAG
  CCGCTGTTGTGACAAGAAAAGCTGTGGCAACCGAAATGAGACTCCCTCAGATCCAGTGAT
  AATTGACAGGTTCTTCTTGAAATTTTTCCTCAAATGTAACCAAAATTGCCTAAAGAATGC
  GGGAAACCCACGTGACATGCGGAGATTCCAGGTCGTGGTGTCTACGACAGTCAATGTGGA
  TGGCCATGTCCTGGCAGTCTCTGATAACATGTTTGTCCATAATAATTCCAAGCATGGGCG
  GAGGGCTCGGAGGCTTGACCCCTCGGAAGGTACGCCCTCTTATCTGGAACATGCTACTCC
  CTGTATCAAAGCCATCAGCCCGAGTGAAGGATGGACGACGGGAGGTGCGACTGTGATCAT
  CATAGGGGACAATTTCTTTGATGGGTTACAGGTCATATTCGGTACCATGCTGGTCTGGAG
  TGAGTTGATCACTCCTCATGCCATCCGTGTGCAGACCCCTCCTCGGCACATCCCTGGTGT
  TGTGGAAGTCACACTGTCCTACAAATCTAAGCAGTTCTGCAAAGGAACACCAGGCAGATT
  CATTTATACAGCGCTCAACGAACCCACCATCGATTATGGTTTCCAGAGGTTACAGAAGGT
  CATTCCTCGGCACCCTGGTGACCCTGAGCGTTTGCCAAAGGAAGTAATACTCAAAAGGGC
  TGCGGATCTGGTAGAAGCACTGTATGGGATGCCACACAACAACCAGGAAATCATTCTGAA
  GAGAGCGGCCGACATTGCCGAGGCCCTGTACAGTGTTCCCCGCAACCACAACCAACTCCC
  GGCCCTTGCTAACACCTCGGTCCACGCAGGGATGATGGGCGTGAATTCGTTCAGTGGACA
  ACTGGCCGTGAATGTCTCCGAGGCATCACAAGCCACCAATCAGGGTTTCACCCGCAACTC
  AAGCAGCGTATCACCACACGGGTACGTGCCGAGCACCACTCCCCAGCAGACCAACTATAA
  CTCCGTCACCACGAGCATGAACGGATACGGCTCTGCCGCAATGTCCAATTTGGGCGGCTC
  CCCCACCTTCCTCAACGGCTCAGCTGCCAACTCCCCCTATGCCATAGTGCCATCCAGCCC
  CACCATGGCCTCCTCCACAAGCCTCCCCTCCAACTGCAGCAGCTCCTCGGGCATCTTCTC
  CTTCTCACCAGCCAACATGGTCTCAGCCGTGAAACAGAAGAGTGCTTTCGCACCAGTCGT
  CAGACCCCAGACCTCCCCACCTCCCACCTGCACCAGCACCAACGGGAACAGCCTGCAAGC
  GATATCTGGCATGATTGTTCCTCCTATGTGAAAGAATTGCCTTGAAGAATTGTATTAATG
  AAGAGGTTGGATTCTGCTACAGAGAGTAATCTGATACAAGTCCCAGAGTGGAACTTTTAA
  CTCAGGCCTTTTTAAGAGGAATCACAATAACTGCAGATTTTTAAACAAACAAAATCACCG
  ACCTTGCAAATACTGAAATTGGAAGAGGGATCTGCAAGTGCAGGGTGTTGGTTAAAGTTG
  TACCTCCCAAGTATTTGGGGGATATATTTATTCTGTATTGACAAAAGCAAATCCACTTTT
  TCTTTTTCTTTTTTTTTTTTAAGCTTAATTCTGCAATCATTTGTCTTTTATAAACCGTAA
  AGCTCTATACAAGGGACACTATAAATAAGACTCCATGTTTTAATTTATGATGTTTTTAAA
  GCTGTGTAAAGGAAGAATGAAGTGGTGATATTTACAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_024007
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024007.2, NP_076870.1
• RefSeq Size: 2762 bp
• RefSeq ORF: 1776 bp
• Locus ID: 1879
• Cytogenetics: 5q33.3
• Protein Families: Transcription Factors
• Gene Summary: ''
  Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is one aa shorter compared to isoform 1.