GRK5 (NM_005308) Human Untagged Clone

CAT#: SC110910

GRK5 (untagged)-Human G protein-coupled receptor kinase 5 (GRK5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRK5
• Synonyms: FP2025; GPRK5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005308 edited
  ATGGAGCTGGAAAACATCGTGGCCAACACGGTCTTGCTGAAAGCCAGGGAAGGGGGCGGA
  GGAAAGCGCAAAGGGAAAAGCAAGAAGTGGAAAGAAATCCTGAAGTTCCCTCACATTAGC
  CAGTGTGAAGACCTCCGAAGGACCATAGACAGAGATTACTGCAGTTTATGTGACAAGCAG
  CCAATCGGGAGGCTGCTTTTCCGGCAGTTTTGTGAAACCAGGCCTGGGCTGGAGTGTTAC
  ATTCAGTTCCTGGACTCCGTGGCAGAATATGAAGTTACTCCAGATGAAAAACTGGGAGAG
  AAAGGGAAGGAAATTATGACCAAGTACCTCACCCCAAAGTCCCCTGTTTTCATAGCCCAA
  GTTGGCCAAGACCTGGTCTCCCAGACGGAGGAGAAGCTCCTACAGAAGCCGTGCAAAGAA
  CTCTTTTCTGCCTGTGCACAGTCTGTCCACGAGTACCTGAGGGGAGAACCATTCCACGAA
  TATCTGGACAGCATGTTTTTTGACCGCTTTCTCCAGTGGAAGTGGTTGGAAAGGCAACCG
  GTGACCAAAAACACTTTCAGGCAGTATCGAGTGCTAGGAAAAGGGGGCTTCGGGGAGGTC
  TGTGCCTGCCAGGTTCGGGCCACGGGTAAAATGTATGCCTGCAAGCGCTTGGAGAAGAAG
  AGGATCAAAAAGAGGAAAGGGGAGTCCATGGCCCTCAATGAGAAGCAGATCCTCGAGAAG
  GTCAACAGTCAGTTTGTGGTCAACCTGGCCTATGCCTACGAGACCAAGGATGCACTGTGC
  TTGGTCCTGACCATCATGAATGGGGGTGACCTGAAGTTCCACATCTACAACATGGGCAAC
  CCTGGCTTCGAGGAGGAGCGGGCCTTGTTTTATGCGGCAGAGATCCTCTGCGGCTTAGAA
  GACCTCCACCGTGAGAACACCGTCTACCGAGATCTGAAACCTGAAAACATCCTGTTAGAT
  GATTATGGCCACATTAGGATCTCAGACCTGGGCTTGGCTGTGAAGATCCCCGAGGGAGAC
  CTGATCCGCGGCCGGGTGGGCACTGTTGGCTACATGGCTCCGGAGGTCCTGAACAACCAG
  AGGTACGGCCTGAGCCCCGACTACTGGGGCCTTGGCTGCCTCATCTATGAGATGATCGAG
  GGCCAGTCGCCGTTCCGCGGCCGCAAGGAGAAGGTGAAGCGGGAGGAGGTGGACCGCCGG
  GTCCTGGAGACGGAGGAGGTGTACTCCCACAAGTTCTCCGAGGAGGCCAAGTCCATCTGC
  AAGATGCTGCTCACGAAAGATGCGAAGCAGAGGCTGGGCTGCCAGGAGGAGGGGGCTGCA
  GAGGTCAAGAGACACCCCTTCTTCAGGAACATGAACTTCAAGCGCTTAGAAGCCGGGATG
  TTGGACCCTCCCTTCGTTCCAGACCCCCGCGCTGTGTACTGTAAGGACGTGCTGGACATC
  GAGCAGTTCTCCACTGTGAAGGGCGTCAATCTGGACCACACAGACGACGACTTCTACTCC
  AAGTTCTCCACGGGCTCTGTGTCCATCCCATGGCAAAACGAGATGATAGAAACAGAATGC
  TTTAAGGAGCTGAACGTGTTTGGACCTAATGGTACCCTCCCGCCAGATCTGAACAGAAAC
  CACCCTCCGGAACCGCCCAAGAAAGGGCTGCTCCAGAGACTCTTCAAGCGGCAGCATCAG
  AACAATTCCAAGAGTTCGCCCAGCTCCAAGACCAGTTTTAACCACCACATAAACTCAAAC
  CATGTCAGCTCGAACTCCACCGGAAGCAGCTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005308 unedited
  NGGTCAAAATTTGTATACGACTCATATAGGCGGCNCGCGAATTCGCACGAGGGCGGTAGG
  CAAGGCGGGCTGCTGGCTCCCCCGGCTCCGGCAGCAGCGGCGGCAGCCCGAGCAGCGGCA
  GCAGCAGCGGCAGCACCCCAGGCGCTGACAGCCCCGCCGGCCGGCTCCGTTGCTGACCGC
  CGACTGTCAATGGAGCTGGAAAACATCGTGGCCAACACGGTCTTGCTGAAAGCCAGGGAA
  GGGGGCGGAGGAAAGCGCAAAGGGAAAAGCAAGAAGTGGAAAGAAATCCTGAAGTTCCCT
  CACATTAGCCAGTGTGAAGACCTCCGAAGGACCATAGACAGAGATTACTGCAGTTTATGT
  GACAAGCAGCCAATCGGGAGGCTGCTTTTCCGGCAGTTTTGTGAAACCAGGCCTGGGCTG
  GAGTGTTACATTCAGTTCCTGGACTCCGTGGCAGAATATGAAGTTACTCCAGATGAAAAA
  CTGGGAGAGAAAGGGAAGGAAATTATGACCAAGTACCTCACCCCAAAGTCCCCTGTTTTC
  ATAGCCCAAGTTGGCCAAGACCTGGTCTCCCAGACGGAGGAGAAGCTCCTACAGAAGCCG
  TGCAAAGAACTCTTTTCTGCCTGTGCACAGTCTGTCCACGAGTACCTGAGGGGAGAACCA
  TTCCACGAATATCTGGACAGCATGTTTTTTGACCGCTNTCTCCAGTGGAAGTGGTTGGAA
  AGGGGCACCGTGACCAAAAACACTNTCAGGCAGTATCGAGTGCTANGAAAAGGNGGCTCG
  GNGAGGTCTGTGCCTGCCAGGTTCGGGCCACGGGTAAAATGTATGCCTGNCAGCGCTGGN
  AGAAGAANAGGGATCAAAAGAGGAAAGGGGAGTTCATGGCCCTCATGAGAAGCAGACCTC
  GAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005308 unedited
  CCCCCCCCAATAGGTTGGACCGTCGGCCGCATCCTAGGAGCGAGTTTTTTTTTTTTTTTT
  TTGATAACCCCCTCCCCGCTAAAATGCCTTGNGAACACATGGCTTAACAGTGGCTGCACA
  GAAAATATACACCCTCAAAGTCTCACTTTCATTTAAATACAAATATACAAAATGTAAACT
  GAGACAGTAGAGAAATTTACAAAACTTTTCTTTAAAAATCATAAAGACAAAATTCAGTTC
  TAGTTATGACGCTATTAAAATACGTGCAAGTTGTCGTATCCAATTGGATTTCTATTGCAA
  TGTTCACCAAAGACAAATCCAATCCACCCCGGCCGGGGCCGCCTCGGAAAACAGACCTGA
  GTGGAAATTTCTTTGAGAAACCTCGAGGTTCTACGTCGACGGGATGGACGGCCTCCTTCC
  CCGGCTCCCGGGGTCTCCCCTGGCAGCCCCACCAGAGCAGGGGCTCCACTACTTCCACTT
  CTAAGGAGAAGGGTCTGGGCTGGTTCCACTGTGGACTTGGAGGCCAGAGCCGAAACTAGC
  TGCTTCCGGTGGAGTTCGAGCTGACATGGTTTGAGTTTATGTGGTGGTTAAAACTGGTCT
  TGGAGCTGGGCGAACTCTTGGAATTGTTCTGATGCTGCCGCTTGAAGAGTCTCTGGAGCA
  GCCCTTTCTTGGGCGGTTCCGGAGGGGTGGTTTCTGTTCAGATCTGGCGGGAGGGTACCA
  TTANGTCCAAACACGTTCAGCTCCTTAAAGCATTCTGTTTCTATCATCTCGTTTTGCCAT
  GGGATGGACACAAGAGCCGTGGTAGAGCTTGAAGTAGAAATCCTTCGCTGGTGTGGTCCA
  GAATGACGCCCTTTACAGTGGAGAACTGGTTCGAGTCCAACACGTTCTTTCAGTAACAGG
  CCCGGGGGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005308
• Insert Size: 2280 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005308.2, NP_005299.1
• RefSeq Size: 2575 bp
• RefSeq ORF: 1773 bp
• Locus ID: 2869
• Cytogenetics: 10q26.11
• Domains: RGS, pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Endocytosis
• Gene Summary: 'This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs). [provided by RefSeq, Jul 2008]'