GRK5 (NM_005308) Human Untagged Clone

CAT#: SC323499

GRK5 (untagged)-Kinase deficient mutant (K215M) of Human G protein-coupled receptor kinase 5 (GRK5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRK5
• Synonyms: FP2025; GPRK5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323499 sequence for NM_005308 edited (data generated by NextGen Sequencing)
  ATGGAGCTGGAAAACATCGTGGCCAACACGGTCTTGCTGAAAGCCAGGGAAGGGGGCGGA
  GGAAAGCGCAAAGGGAAAAGCAAGAAGTGGAAAGAAATCCTGAAGTTCCCTCACATTAGC
  CAGTGTGAAGACCTCCGAAGGACCATAGACAGAGATTACTGCAGTTTATGTGACAAGCAG
  CCAATCGGGAGGCTGCTTTTCCGGCAGTTTTGTGAAACCAGGCCTGGGCTGGAGTGTTAC
  ATTCAGTTCCTGGACTCCGTGGCAGAATATGAAGTTACTCCAGATGAAAAACTGGGAGAG
  AAAGGGAAGGAAATTATGACCAAGTACCTCACCCCAAAGTCCCCTGTTTTCATAGCCCAA
  GTTGGCCAAGACCTGGTCTCCCAGACGGAGGAGAAGCTCCTACAGAAGCCGTGCAAAGAA
  CTCTTTTCTGCCTGTGCACAGTCTGTCCACGAGTACCTGAGGGGAGAACCATTCCACGAA
  TATCTGGACAGCATGTTTTTTGACCGCTTTCTCCAGTGGAAGTGGTTGGAAAGGCAACCG
  GTGACCAAAAACACTTTCAGGCAGTATCGAGTGCTAGGAAAAGGGGGCTTCGGGGAGGTC
  TGTGCCTGCCAGGTTCGGGCCACGGGTAAAATGTATGCCTGCATGCGCTTGGAGAAGAAG
  AGGATCAAAAAGAGGAAAGGGGAGTCCATGGCCCTCAATGAGAAGCAGATCCTCGAGAAG
  GTCAACAGTCAGTTTGTGGTCAACCTGGCCTATGCCTACGAGACCAAGGATGCACTGTGC
  TTGGTCCTGACCATCATGAATGGGGGTGACCTGAAGTTCCACATCTACAACATGGGCAAC
  CCTGGCTTCGAGGAGGAGCGGGCCTTGTTTTATGCGGCAGAGATCCTCTGCGGCTTAGAA
  GACCTCCACCGTGAGAACACCGTCTACCGAGATCTGAAACCTGAAAACATCCTGTTAGAT
  GATTATGGCCACATTAGGATCTCAGACCTGGGCTTGGCTGTGAAGATCCCCGAGGGAGAC
  CTGATCCGCGGCCGGGTGGGCACTGTTGGCTACATGGCTCCAGAGGTCCTGAACAACCAG
  AGGTACGGCCTGAGCCCCGACTACTGGGGCCTTGGCTGCCTCATCTATGAGATGATCGAG
  GGCCAGTCGCCGTTCCGCGGCCGCAAGGAGAAGGTGAAGCGGGAGGAGGTGGACCGCCGG
  GTCCTGGAGACGGAGGAGGTGTACTCCCACAAGTTCTCCGAGGAGGCCAAGTCCATCTGC
  AAGATGCTGCTCACGAAAGATGCGAAGCAGAGGCTGGGCTGCCAGGAGGAGGGGGCTGCA
  GAGGTCAAGAGACACCCCTTCTTCAGGAACATGAACTTCAAGCGCTTAGAAGCCGGGATG
  TTGGACCCTCCCTTCGTTCCAGACCCCCGCGCTGTGTACTGTAAGGACGTGCTGGACATC
  GAGCAGTTCTCCACTGTGAAGGGCGTCAATCTGGACCACACAGACGACGACTTCTACTCC
  AAGTTCTCCACGGGCTCTGTGTCCATCCCATGGCAAAACGAGATGATAGAAACAGAATGC
  TTTAAGGAGCTGAACGTGTTTGGACCTAATGGTACCCTCCCGCCAGATCTGAACAGAAAC
  CACCCTCCGGAACCGCCCAAGAAAGGGCTGCTCCAGAGACTCTTCAAGCGGCAGCATCAG
  AACAATTCCAAGAGTTCGCCCAGCTCCAAGACCAGTTTTAACCACCACATAAACTCAAAC
  CATGTCAGCTCGAACTCCACCGGAAGCAGCTAG
  
  Clone variation with respect to NM_005308.2
  644 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_005308 unedited
  CGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC
  CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGTAGGCAAGGCG
  GGCTGCTGGCTCCCCCGGCTCCGGCAGCAGCGGCGGCAGCCCGAGCAGCGGCAGCAGCAGCGGCAGCACC
  CCAGGCGCTGACAGCCCCGCCGGCCGGCTCCGTTGCTGACCGCCGACTGTCAATGGAGCTGGAAAACATC
  GTGGCCAACACGGTCTTGCTGAAAGCCAGGGAAGGGGGCGGAGGAAAGCGCAAAGGGAAAAGCAAGAAGT
  GGAAAGAAATCCTGAGGTTCCCTCACATTAGCCAGTGTGAAGACCTCCGAAGGACAATAGACAAGAAAAT
  AACCTGGCAAGTTATATGTGACCAAGCCAGCCAATCCGGGAGGGCTTGCTTTTTCCGGCAGTTTTGGTGA
  AACCCAGGCCTGGGGCTGGAGATGGTTACATTCAGTTCCGGACTCCCGGTGGGAAAATAATGAGGTAATT
  CCAAATGAAAACTGGGGAGAAAAAGGGAAGGGAATTTTTGACAGTTACCTACCCCAAGGTCCCCGTGTTT
  TCATAGCCAGTTTGCCCAGCCTGGGTCTCCCAAAGGGGAGAAACTCTCCTACAAACGCTGCGCAGAGATC
  TTTTCTGTGTGTGCGCACACTGTGTCCAAGACCCGAGGGGGAACACTTCTCCCAATATTGTCGACACGTG
  TTTGACGCGTTTCCACTGTAGAAGGTGGTGAGAGGCCAGTGTCACACAACACTCTTGCGCATTCATAGCT
  CAGAAAGGGCTCTGCGAGACTGTCGTGCCAAATTGCAACGGGAATATGACCCTCGCTTCGGAAAGAAAAC
  
  Kinase domain sequence

  >SC323499 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  GMTGGTGACAAAACACTTTMGGCAGTATCGAGTGCTAGGAAAAGGGGGCTTCGGGGAGGTCTGTGCCTGC
  CAGGTTCGGGCCACGGGTAAAATGTATGCCTGCATGCGCTTGGAGAAGAAGAGGATCAAAAAGAGGAAAG
  GGGAGTCCATGGCCCTCAATGAGAAGCAGATCCTCGAGAAGGTCAACAGTCAGTTTGTGGTCAACCTGGC
  CTATGCCTACGAGACCAAGGATGCACTGTGCTTGGTCCTGACCAT
  
• Restriction Sites: Please inquire
• ACCN: NM_005308
• Insert Size: 2280 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005308.2, NP_005299.1
• RefSeq Size: 2575 bp
• RefSeq ORF: 1773 bp
• Locus ID: 2869
• Cytogenetics: 10q26.11
• Domains: RGS, pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Endocytosis
• Gene Summary: 'This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs). [provided by RefSeq, Jul 2008]'