MCM6 (NM_005915) Human Untagged Clone

CAT#: SC110945

MCM6 (untagged)-Human minichromosome maintenance complex component 6 (MCM6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MCM6
• Synonyms: MCG40308; Mis5; P105MCM
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110945 sequence for NM_005915 edited (data generated by NextGen Sequencing)
  ATGGACCTCGCGGCGGCAGCGGAGCCGGGCGCCGGCAGCCAGCACCTGGAGGTCCGCGAC
  GAGGTGGCCGAGAAGTGCCAGAAACTGTTCCTGGACTTCTTGGAGGAGTTTCAGAGCAGC
  GATGGAGAAATTAAATACTTGCAATTAGCAGAGGAACTGATTCGTCCTGAGAGAAACACA
  TTGGTTGTGAGTTTTGTGGACCTGGAACAATTTAACCAGCAACTTTCCACCACCATTCAA
  GAGGAGTTCTATAGAGTTTACCCTTACCTGTGTCGGGCCTTGAAAACATTCGTCAAAGAC
  CGTAAAGAGATCCCTCTTGCCAAGGATTTTTATGTTGCATTCCAAGACCTGCCTACCAGA
  CACAAGATTCGAGAGCTCACCTCATCCAGAATTGGTTTGCTCACTCGCATCAGTGGGCAG
  GTGGTGCGGACTCACCCAGTTCACCCAGAGCTTGTGAGCGGAACTTTTCTGTGCTTGGAC
  TGTCAGACAGTGATCAGGGATGTAGAACAGCAGTTCAAATACACACAGCCAAACATCTGC
  CGAAATCCAGTTTGTGCCAACAGGAGGAGATTCTTACTGGATACAAATAAATCAAGATTT
  GTTGATTTTCAAAAGGTTCGTATTCAAGAGACCCAAGCTGAGCTTCCTCGAGGGAGTATC
  CCCCGCAGTTTAGAAGTAATTTTAAGGGCTGAAGCTGTGGAATCAGCTCAAGCTGGTGAC
  AAGTGTGACTTTACAGGGACACTGATTGTTGTGCCTGACGTCTCCAAGCTTAGCACACCA
  GGAGCACGTGCAGAAACTAATTCCCGTGTCAGTGGTGTTGATGGATATGAGACAGAAGGC
  ATTCGAGGACTCCGGGCCCTTGGTGTTAGGGACCTTTCTTATAGGCTGGTCTTTCTTGCC
  TGCTGTGTTGCGCCAACCAACCCAAGGTTTGGGGGGAAAGAGCTCAGAGATGAGGAACAG
  ACAGCTGAGAGCATTAAGAACCAAATGACTGTGAAAGAATGGGAGAAAGTGTTTGAGATG
  AGTCAAGATAAAAATCTATACCACAATCTTTGTACCAGCCTGTTCCCTACTATACATGGC
  AATGATGAAGTAAAACGGGGTGTCCTGCTGATGCTCTTTGGTGGCGTTCCAAAGACAACA
  GGAGAAGGGACCTCTCTTCGAGGGGACATAAATGTTTGCATTGTTGGTGACCCAAGTACA
  GCTAAGAGCCAATTTCTCAAGCACGTGGAGGAGTTCAGCCCCAGAGCTGTCTACACCAGT
  GGTAAAGCGTCCAGTGCTGCTGGCTTAACAGCAGCTGTTGTGAGAGATGAAGAATCTCAT
  GAGTTTGTCATTGAGGCTGGAGCTTTGATGTTGGCTGATAATGGTGTGTGTTGTATTGAT
  GAATTTGATAAGATGGACGTGCGGGATCAAGTTGCTATTCATGAAGCTATGGAACAGCAG
  ACCATATCCATCACTAAAGCAGGAGTGAAGGCTACTCTGAACGCCCGGACGTCCATTTTG
  GCAGCAGCAAACCCAATCAGTGGACACTATGACAGATCAAAATCATTGAAACAGAATATA
  AATTTGTCAGCTCCCATCATGTCCCGATTCGATCTCTTCTTTATCCTTGTGGATGAATGT
  AATGAGGTTACAGATTATGCCATTGCCAGGCGCATAGTAGATTTGCATTCAAGAATTGAG
  GAATCAATTGATCGTGTCTATTCCCTCGATGATATCAGAAGATATCTTCTCTTTGCAAGA
  CAGTTTAAACCCAAGATTTCCAAAGAGTCAGAGGACTTCATTGTGGAGCAATATAAACAT
  CTCCGCCAGAGAGATGGTTCTGGAGTGACCAAGTCTTCATGGAGGATTACAGTGCGACAG
  CTTGAGAGCATGATTCGTCTCTCTGAAGCTATGGCTCGGATGCACTGCTGTGATGAGGTC
  CAACCTAAACATGTGAAGGAAGCTTTCCGGTTACTGAATAAATCAATCATCCGTGTGGAA
  ACACCTGATGTCAATCTAGATCAAGAGGAAGAGATCCAGATGGAGGTAGATGAGGGTGCT
  GGTGGCATCAATGGTCATGCTGACAGCCCTGCTCCTGTGAACGGGATCAATGGCTACAAT
  GAAGACATAAATCAAGAGTCTGCTCCCAAAGCCTCCTTAAGGCTGGGCTTCTCTGAGTAC
  TGCCGAATCTCTAACCTTATTGTGCTTCACCTCAGAAAGGTGGAAGAAGAAGAGGACGAG
  TCAGCATTAAAGAGGAGCGAGCTTGTTAACTGGTACTTGAAGGAAATCGAATCAGAGATA
  GACTCTGAAGAAGAACTTATAAATAAAAAAAGAATCATAGAGAAAGTTATTCATCGACTC
  ACACACTATGATCATGTTCTAATTGAGCTCACCCAGGCTGGATTGAAAGGCTCCACAGAG
  GGAAGTGAGAGCTATGAAGAAGATCCCTACTTGGTAGTTAACCCTAACTACTTGCTCGAA
  GATTGA
  
  Clone variation with respect to NM_005915.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005915 unedited
  NATTTTGTAATACGACTCACTATAGGGGCGGCCGCGAATTCGCACGAGGGCGGCGCGCGC
  AAAGCTGCAGCGTCTGGAAAAAACGACTTGTGGCGGTCGAGCGTGGCGCAGGCGAATCCT
  CGGCACTAAGCAAATATGGACCTCGCGGCGGCAGCGGAGCCGGGCGCCGGCAGCCAGCAC
  CTGGAGGTCCGCGACGAGGTGGCCGAGAAGTGCCAGAAACTGTTCCTGGACTTCTTGGAG
  GAGTTTCAGAGCAGCGATGGAGAAATTAAATACTTGCAATTAGCAGAGGAACTGATTCGT
  CCTGAGAGAAACACATTGGTTGTGAGTTTTGTGGACCTGGAACAATTTAACCAGCAACTT
  TCCACCACCATTCAAGAGGAGTTCTATAGAGTTTACCCTTACCTGTGTCGGGCCTTGAAA
  ACATTCGTCAAAGACCGTAAAGAGATCCCTCTTGCCAAGGATTTTTATGTTGCATTCCAA
  GACCTGCCTACCAGACACAAGATTCGAGAGCTCACCTCATCCAGAATTGGTTTGCTCACT
  CGCATCAGTGGGCAGGTGGTGCGGACTCACCCAGTTCACCCAGAGCTTGTGAGCGGAACT
  TTTCTGTGCTTGGACTGTCAGACAGTGATCAGGGATGTAGAACAGCAGTTCAAATACACA
  CAGCCAAACATCTGCCGAAATCCAGTTTGTGCCAACAGGAGGAGATTCTTACTGGATACA
  NNATAATCAAGATTTGTTGATTTTCAAAAGGGTCGTATTCAAGAGACCCCAGCTGAGCTT
  CCTCGAGGGAGTATCCCCCGCAGTTTAGAAGTAATTTTAGGGCTGAAGCTGTGGAATCAG
  CTCAAGCTGGTGACCAGTGTGACTTTAAGGGACACCTGATGTTGTGCCTGACGTCTCCAA
  GCTTACACCACCAGAGCCGTGCAGAAACTAANTCCCTGTCACGGTGGTGATGGAATGAAA
  CCAAGGCTTCCAGACTCCGCCCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005915 unedited
  GGACCGCGGCACGCAATCTATAGTCGAGNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTAAAAACAAGTTTTTTTATTTTGATAAATAAAAGCAGTACCTGGC
  ATTGGAAAAAAAGTTTTTTGCTCAATAGGACAGCACATCCCTTAAAAGTAAGGGGCCTTT
  TTTTACACATGAAAAACAAAGGTTTGGGTTATAAAAACTACACTTTTTTGGGTTCCAACT
  TCCCTGGGACAGGAAACACCCCAAAGGAAGGGCTGAACCCTGGGTGGGTATAAAACCTGG
  GATGAATGGGACACATAGGACCTTCAACTCATTTCTTGTTTCTGAAAACAAATCCGGGAA
  GCATCACTTCCAAAAACATTTCTGCCCCTAAAAAAGCTCCAGCCAGGCTCCAGGCCACGA
  GGGGCTGGGCCACAGTTCCTCAGCTCTGGCCAGTTACTTTCACTATCTAAATCTTCAAGC
  AAGTATTTAGGGTTAACTACCAAGTAGGGATCTTCTTCATAGCTCTCACTTCCCTCGGGG
  GAGCCTTTCAATCCAGCCGGGGGGAGCTCAATTAAAACATGATCATAGGGGGGGAGCCGA
  TAAATAACTTTCTCTATGATCCTTTTTTTATTAATAAGCTCTTCTTAAAAGTCTATCTCT
  GATTCGATTTCCTTCAAGCACCAGTTAACAAGCTCGCTCCTCTTTAATGCAGACTTGGCC
  TCTTCCTTCTACACCCTTTGTGAGGGGAACCACATAAGGTAGAGATTCGGCAGTCCTCAA
  AAACCCCACCCTTAAGGAGGCTTGGGAGCAAACTCTTGATTTATGTCTTCTTTGAACCAT
  TGATCCCGTTCCAAGAACAGGGCTGCANCATAACCTTGGTGCCACCAACACCCTATTCAC
  CTCATTGGGATTTCTTCCTCGGATCTAAATGAAACAGGGGTTCCACCCGTTGAATGATTT
  TTTAGTACGCGAGCCTCCTCCATGTTAGGGGAACCTATCCAGAAGGCTCCACCATATTTA
  AG
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_005915
• ORF Size: 2466 bp
• Insert Size: 3080
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_005915.4, NP_005906.2
• RefSeq Size: 3769
• RefSeq ORF: 2466
• Locus ID: 4175
• Domains: MCM
• Protein Families: Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle, DNA replication
• Gene Summary: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication. Single nucleotide polymorphisms in the intron regions of this gene are associated with differential transcriptional activation of the promoter of the neighboring lactase gene and, thereby, influence lactose intolerance in early adulthood. [provided by RefSeq, May 2012]