MCM6 (NM_005915) Human Untagged Clone

CAT#: SC323955

MCM6 (untagged)-Human minichromosome maintenance complex component 6 (MCM6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MCM6
• Synonyms: MCG40308; Mis5; P105MCM
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005915.4 CCACGCGTCCGGTGGCGGTCGAGCGTGGCGTAGGCGAATCCTCGGCACTAAGCATATGGA
  CCTCGCGGCGGCAGCGGAGCCGGGCGCCGGCAGCCAGCACCTGGAGGTCCGCGACGAGGT
  GGCCGAGAAGTGCCAGAAACTGTTCCTGGACTTCTTGGAGGAGTTTCAGAGCAGCGATGG
  AGAAATTAAATACTTGCAATTAGCAGAGGAACTGATTCGTCCTGAGAGAAACACATTGGT
  TGTGAGTTTTGTGGACCTGGAACAATTTAACCAGCAACTTTCCACCACCATTCAAGAGGA
  GTTCTATAGAGTTTACCCTTACCTGTGTCGGGCCTTGAAAACATTCGTCAAAGACCGTAA
  AGAGATCCCTCTTGCCAAGGATTTTTATGTTGCATTCCAAGACCTGCCTACCAGACACAA
  GATTCGAGAGCTCACCTCATCCAGAATTGGTTTGCTCACTCGCATCAGTGGGCAGGTGGT
  GCGGACTCACCCAGTTCACCCAGAGCTTGTGAGCGGAACTTTTCTGTGCTTGGACTGTCA
  GACAGTGATCAGGGATGTAGAACAGCAGTTCAAATACACACAGCCAAACATCTGCCGAAA
  TCCAGTTTGTGCCAACAGGAGGAGATTCTTACTGGATACAAATAAATCAAGATTTGTTGA
  TTTTCAAAAGGTTCGTATTCAAGAGACCCAAGCTGAGCTTCCTCGAGGGAGTATCCCCCG
  CAGTTTAGAAGTAATTTTAAGGGCTGAAGCTGTGGAATCAGCTCAAGCTGGTGACAAGTG
  TGACTTTACAGGGACACTGATTGTTGTGCCTGACGTCTCCAAGCTTAGCACACCAGGAGC
  ACGTGCAGAAACTAATTCCCGTGTCAGTGGTGTTGATGGATATGAGACAGAAGGCATTCG
  AGGACTCCGGGCCCTTGGTGTTAGGGACCTTTCTTATAGGCTGGTCTTTCTTGCCTGCTG
  TGTTGCGCCAACCAACCCAAGGTTTGGGGGGAAAGAGCTCAGAGATGAGGAACAGACAGC
  TGAGAGCATTAAGAACCAAATGACTGTGAAAGAATGGGAGAAAGTGTTTGAGATGAGTCA
  AGATAAAAATCTATACCACAATCTTTGTACCAGCCTGTTCCCTACTATACATGGCAATGA
  TGAAGTAAAACGGGGTGTCCTGCTGATGCTCTTTGGTGGCGTTCCAAAGACAACAGGAGA
  AGGGACCTCTCTTCGAGGGGACATAAATGTTTGCATTGTTGGTGACCCAAGTACAGCTAA
  GAGCCAATTTCTCAAGCACGTGGAGGAGTTCAGCCCCAGAGCTGTCTACACCAGTGGTAA
  AGCGTCCAGTGCTGCTGGCTTAACAGCAGCTGTTGTGAGAGATGAAGAATCTCATGAGTT
  TGTCATTGAGGCTGGAGCTTTGATGTTGGCTGATAATGGTGTGTGTTGTATTGATGAATT
  TGATAAGATGGACGTGCGGGATCAAGTTGCTATTCATGAAGCTATGGAACAGCAGACCAT
  ATCCATCACTAAAGCAGGAGTGAAGGCTACTCTGAACGCCCGGACGTCCATTTTGGCAGC
  AGCAAACCCAATCAGTGGACACTATGACAGATCAAAATCATTGAAACAGAATATAAATTT
  GTCAGCTCCCATCATGTCCCGATTCGATCTCTTCTTTATCCTTGTGGATGAATGTAATGA
  GGTTACAGATTATGCCATTGCCAGGCGCATAGTAGATTTGCATTCAAGAATTGAGGAATC
  AATTGATCGTGTCTATTCCCTCGATGATATCAGAAGATATCTTCTCTTTGCAAGACAGTT
  TAAACCCAAGATTTCCAAAGAGTCAGAGGACTTCATTGTGGAGCAATATAAACATCTCCG
  CCAGAGAGATGGTTCTGGAGTGACCAAGTCTTCATGGAGGATTACAGTGCGACAGCTTGA
  GAGCATGATTCGTCTCTCTGAAGCTATGGCTCGGATGCACTGCTGTGATGAGGTCCAACC
  TAAACATGTGAAGGAAGCTTTCCGGTTACTGAATAAATCAATCATCCGTGTGGAAACACC
  TGATGTCAATCTAGATCAAGAGGAAGAGATCCAGATGGAGGTAGATGAGGGTGCCGGTGG
  CATCAATGGTCATGCTGACAGCCCTGCTCCTGTGAACGGGATCAATGGCTACAATGAAGA
  CATAAATCAAGAGTCTGCTCCCAAAGCCTCCTTAAGGCTGGGCTTCTCTGAGTACTGCCG
  AATCTCTAACCTTATTGTGCTTCACCTCAGAAAGGTGGAAGAAGAAGAGGACGAGTCAGC
  ATTAAAGAGGAGCGAGCTTGTTAACTGGTACTTGAAGGAAATCGAATCAGAGATAGACTC
  TGAAGAAGAACTTATAAATAAAAAAAGAATCATAGAGAAAGTTATTCATCGACTCACACA
  CTATGATCATGTTCTAATTGAGCTCACCCAGGCTGGATTGAAAGGCTCCACAGAGGGAAG
  TGAGAGCTATGAAGAAGATCCCTACTTGGTAGTTAACCCTAACTACTTGCTCGAAGATTG
  AGATAGTGAAAGTAACTGACCAGAGCTGAGGAACTGTGGCACAGCACCTCGTGGCCTGGA
  GCCTGGCTGGAGCTCTGCTAGGGACAGAAGTGTTTCTGGAAGTGATGCTTCCAGGATTTG
  TTTTCAGAAACAAGAATTGAGTTGATGGTCCTATGTGTCACATTCATCACAGGTTTCATA
  CCAACACAGGCTTCAGCACTTCCTTTGGTGTGTTTCCTGTCCCAGTGAAGTTGGAACCAA
  ATAATGTGTAGTCTCTATAACCAATACCTTTGTTTTCATGTGTAAGAAAAGGCCCATTAC
  TTTTAAGGTATGTGCTGTCCTATTGAGCAAATAACTTTTTTTCAATTGCCAGCTACTGCT
  TTTATTCATCAAAATAAAATAACTTGTTCTGAAGTTGTCTATTGGATTTCTTTCTACTGT
  ACCCTGATTATTACTTCCATCTACTTCTGAATGTGAGACTTTCCCTTTTTGCTTAACCTG
  GAGTGAAGAGGTAGAACTGTGGTATTATGGATGAGGTTTCTATGAGAAGGAGTCATTAGA
  GAACTCATATGAAAGCTAGAGGCCTTAGAGATGACTTTCCAAGGTTAATTCCAGTTTTTT
  TTTTTTTTAAGTTTATAAAAGTTTATTATACTTTTTTAAAATTACTCTTTAGTAATTTAT
  TTTACTTCTGTGTCCTAAGGGTAATTTCTCAGGATTGTTTTCAAATTGCTTTTTTAGGGG
  AAATAGGTCATTTGCTATATTACAAGCAATCCCCAAATTTTATGGTCTTCCAGGAAAAGT
  TATTACCGTTTATGATACTAACAGTTCCTGAGACTTAGCTATGATCAGTATGTTCATGAG
  GTGGAGCAGTTCCTGTGTTGCAGCTTTTAACAACAGATGGCATTCATTAAATCACAAAGT
  ATGTTAAAGGTCACAAAAGCAAAATAACTGTCTGAGGCTAAGGCCCACGTGGGACAGTCT
  AATACCCATGAGTACTCAACTTGCCTTGATGTCTGAGCTTTCCAGTGCAATGTGAATTTG
  AGCAGCCAGAAATCTATTAGTAGAAAGCAAGACAGATTAATATAGGTTAAAACAATGATT
  TAAATATGTTTCTCCCAATAATTATCTCTTTCCCTGGAATCAACTTGTATGAAACCTTGT
  CAAAATGTACTCCACAAGTATGTACAATTAAGTATTTTAAAAATAAATGGCAAACATTAA
  AAAAAAAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005915.4 unedited
  TCAATTGTATACGACTCCTATAGGGCGGCCGGCGATCGCGGCGCGCCGGTACCGGTCCGG
  AATTCCCGGGATATCGTCGACCCACGCGTCCGGTGGCGGTCGAGCGTGGCGTAGGCGAAT
  CCTCGGCACTAAGCATATGGACCTCGCGGCGGCAGCGGAGCCGGGCGCCGGCAGCCAGCA
  CCTGGAGGTCCGCGACGAGGTGGCCGAGAAGTGCCAGAAACTGTTCCTGGACTTCTTGGA
  GGAGTTTCAGAGCAGCGATGGAGAAATTAAATACTTGCAATTAGCAGAGGAACTGATTCG
  TCCTGAGAGAAACACATTGGTTGTGAGTTTTGTGGACCTGGAACAATTTAACCAGCAACT
  TTCCACCACCATTCAAGAGGAGTTCTATAGAGTTTACCCTTACCTGTGTCGGGCCTTGAA
  AACATTCGTCAAAGACCGTAAAGAGATCCCTCTTGCCAAGGATTTTTATGTTGCATTCCA
  AGACCTGCCTACCAGACACAAGATTCGAGAGCTCACCTCATCCAGAATTGGTTTGCTCAC
  TCGCATCAGTGGGCAGGTGGTGCGGACTCACCCAGTTCACCCAGAGCTTGTGAGCGGAAC
  TTTTCTGTGCTTGGACTGTCAGACAGTGATCAGGGATGTAGAACAGCAGTTCAAATACAC
  ACAGCCAAACATCTGCCGAAATCCAGTTTGTGCCAACAGGAGGAGATTCTTACTGGATAC
  AAATAAATCAAGATTTGTTGATTTTCAAAAGGTTCGTATTCAAGAGACCCAAGCTGAGCT
  TCCTCGAGGGAGTATCCCCCGCAGTTTAGAAGTAATTTTAAGGGCTGAAGCTGTGGAATC
  AGCTCAAGCTGGTGACAGTGTGACTTTACAGGGAACACTGATTGTTGTGCCTGACGTTCT
  CAGCTAGCACACAGGAGGCACGTGCAGGAAACATATCCCGGTGTCAGTGGTGTGATGATA
  TTGAGACAGAAGCATTTCGAGGAACTCGGACCTGTGTGTGTTAGGGC
• Restriction Sites: ECoRI-NOT
• ACCN: NM_005915
• ORF Size: 2466 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_005915.4, NP_005906.2
• RefSeq Size: 3769
• RefSeq ORF: 2466
• Locus ID: 4175
• Domains: MCM
• Protein Families: Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle, DNA replication
• Gene Summary: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication. Single nucleotide polymorphisms in the intron regions of this gene are associated with differential transcriptional activation of the promoter of the neighboring lactase gene and, thereby, influence lactose intolerance in early adulthood. [provided by RefSeq, May 2012]