METT10D (METTL16) (NM_024086) Human Untagged Clone

CAT#: SC112272

METTL16 (untagged)-Human methyltransferase like 16 (METTL16)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: METTL16
• Synonyms: METT10D
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112272 sequence for NM_024086 edited (data generated by NextGen Sequencing)
  ATGGCTCTGAGTAAATCAATGCATGCAAGAAATAGATACAAGGACAAACCTCCTGACTTT
  GCATATCTGGCATCCAAATATCCAGATTTTAAGCAGCATGTTCAGATAAATCTGAATGGA
  AGAGTGAGCCTTAATTTTAAAGACCCCGAAGCAGTCAGAGCTCTGACGTGTACTCTCCTA
  AGGGAAGATTTTGGACTTTCTATTGATATTCCATTGGAGAGACTAATTCCCACAGTTCCC
  TTGAGACTCAACTATATTCACTGGGTAGAAGATCTGATCGGTCACCAGGATTCTGACAAA
  AGTACTCTCCGAAGAGGAATTGACATAGGCACGGGGGCATCTTGCATCTACCCCTTACTT
  GGAGCAACCTTGAATGGCTGGTATTTCCTCGCAACAGAAGTGGATGATATGTGTTTCAAC
  TATGCAAAGAAAAATGTGGAACAGAATAACTTATCTGATCTCATAAAAGTGGTGAAAGTG
  CCACAGAAGACACTCCTGATGGATGCTCTTAAAGAAGAATCTGAGATAATCTATGACTTT
  TGCATGTGCAACCCTCCCTTTTTTGCCAATCAATTGGAAGCCAAGGGAGTAAACTCACGA
  AATCCTCGAAGACCTCCGCCTAGTTCTGTTAATACAGGAGGCATCACAGAGATCATGGCA
  GAAGGAGGTGAATTAGAGTTTGTTAAAAGGATCATCCATGACAGTCTACAACTTAAAAAA
  AGATTAAGATGGTATAGCTGCATGCTGGGAAAGAAATGCAGCCTGGCGCCTCTGAAGGAG
  GAGCTTCGCATACAAGGGGTTCCCAAAGTAACGTACACTGAATTCTGTCAAGGTCGGACA
  ATGAGATGGGCCTTAGCTTGGAGTTTTTATGATGATGTCACAGTACCATCACCACCAAGT
  AAGCGAAGAAAATTAGAGAAACCGAGAAAACCCATAACATTCGTGGTGCTGGCGTCCGTG
  ATGAAGGAATTATCCCTCAAAGCATCACCTCTGCGCTCGGAGACGGCGGAAGGCATAGTC
  GTTGTCACGACATGGATTGAAAAAATTCTCACTGATTTGAAGGTCCAGCATAAACGAGTT
  CCCTGTGGAAAAGAGGAAGTCAGCCTTTTCCTAACGGCCATAGAAAACTCCTGGATTCAT
  TTAAGGAGAAAGAAAAGAGAGCGTGTGAGACAGCTGAGAGAAGTTCCCCGAGCTCCTGAG
  GACGTCATTCAGGCCTTGGAAGAGAAAAAGCCCACCCCCAAAGAGTCTGGCAATAGCCAA
  GAACTGGCCAGGGGCCCCCAGGAGAGGACCCCCTGTGGGCCTGCTCTGCGGGAAGGCGAG
  GCTGCCGCTGTGGAGGGCCCGTGCCCGAGCCAGGAGTCCCTGTCCCAGGAGGAAAACCCG
  GAACCCACGGAGGATGAAAGGAGTGAGGAAAAGGGAGGGGTGGAGGTTTTGGAAAGTTGT
  CAAGGCTCTAGCAACGGAGCCCAGGACCAAGAGGCTTCTGAGCAGTTCGGCAGCCCAGTG
  GCTGAAAGGGGGAAACGTCTCCCAGGAGTGGCCGGACAGTACCTGTTTAAGTGTTTGATA
  AACGTTAAGAAGGAGGTGGACGATGCCTTAGTGGAGATGCACTGGGTTGAGGGCCAGAAC
  AGGGATCTGATGAACCAGCTTTGCACCTACATACGTAACCAAATTTTCAGGCTTGTTGCA
  GTTAACTAG
  
  Clone variation with respect to NM_024086.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024086 unedited
  TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTGTACAGGCCCACGCCAGA
  CACGACGTCTGGCAGGAACCTCGGCCTCAGAGATGGCTCTGAGTAAATCAATGCATGCAA
  GAAATAGATACAAGGACAAACCTCCTGACTTTGCATATCTGGCATCCAAATATCCAGATT
  TTAAGCAGCATGTTCAGATAAATCTGAATGGAAGAGTGAGCCTTAATTTTAAAGACCCCG
  AAGCAGTCAGAGCTCTGACGTGTACTCTCCTAAGGGAAGATTTTGGACTTTCTATTGATA
  TTCCATTGGAGAGACTAATTCCCACAGTTCCCTTGAGACTCAACTATATTCACTGGGTAG
  AAGATCTGATCGGTCACCAGGATTCTGACAAAAGTACTCTCCGAAGAGGAATTGACATAG
  GCACGGGGGCATCTTGCATCTACCCCTTACTTGGAGCAACCTTGAATGGCTGGTATTTCC
  TCGCAACAGAAGTGGATGATATGTGTTTCAACTATGCAAAGAAAAATGTGGAACAGAATA
  ACTTATCTGATCTCATAAAAGTGGTGAAAGTGCCACAGAAGACACTCCTGATGGATGCTC
  TTAAAGAAGAATCTGAGATAATCTATGACTTTTGCATGTGCAACCCTCCCTTTTTTGCCA
  ATCAATTGGAAGCCAAGGGAGTAAACTCACGAAATCCTCGAAGACCTNCGCCTAGTTCTG
  TTAATACAGGANGCATCACAGAGATCATGGCAGAAGGAGGGTGAANTTAGAGTTGNTAAA
  AGGNATCATNCATGACAGTCTACAACTTAAAAAAAGATNAAGATGTATAGCTGCATGCTG
  GGAAAGAAAGCAGCCTGGCGCCTCTGAAGGNAGAGCTCGCATACAGGNGNTCCNNAAGTA
  CGTCACTGAATCTGTCAGGTCGACATGAGATGGCCCTAGCTGAGTTTATGAGAGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_024086
• ORF Size: 1515 bp
• Insert Size: 3670
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_024086.2, NP_076991.2
• RefSeq Size: 3368
• RefSeq ORF: 1515
• Locus ID: 79066
• Gene Summary: RNA N6-methyltransferase that methylates adenosine residues of a subset of RNAs and plays a key role in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts (PubMed:28525753). Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs) (PubMed:28525753). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure (PubMed:28525753). In presence of S-adenosyl-L-methionine, binds the 3'-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, leading to intron retention and preventing MAT2A mRNA splicing (PubMed:28525753). In S-adenosyl-L-methionine-limiting conditions, binds the 3'-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, leading to stimulate splicing of the MAT2A retained intron, and promoting expression of MAT2A (PubMed:28525753). In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA): specifically N6-methylates adenine in position 43 of U6 snRNAs (PubMed:28525753). Specifically binds the 3'-end of the MALAT1 long non-coding RNA (PubMed:27872311). [UniProtKB/Swiss-Prot Function]