METT10D (METTL16) (NM_024086) Human Untagged Clone

CAT#: SC317966

METTL16 (untagged)-Human methyltransferase like 16 (METTL16)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: METTL16
• Synonyms: METT10D
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_024086, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTCTGAGTAAATCAATGCATGCAAGAAATAGATACAAGGACAAACCTCCTGACTTTGCATATCTGG
  CATCCAAATATCCAGATTTTAAGCAGCATGTTCAGATAAATCTGAATGGAAGAGTGAGCCTTAATTTTAA
  AGACCCCGAAGCAGTCAGAGCTCTGACGTGTACTCTCCTAAGGGAAGATTTTGGACTTTCTATTGATATT
  CCATTGGAGAGACTAATTCCCACAGTTCCCTTGAGACTCAACTATATTCACTGGGTAGAAGATCTGATCG
  GTCACCAGGATTCTGACAAAAGTACTCTCCGAAGAGGAATTGACATAGGCACGGGGGCATCTTGCATCTA
  CCCCTTACTTGGAGCAACCTTGAATGGCTGGTATTTCCTCGCAACAGAAGTGGATGATATGTGTTTCAAC
  TATGCAAAGAAAAATGTGGAACAGAATAACTTATCTGATCTCATAAAAGTGGTGAAAGTGCCACAGAAGA
  CACTCCTGATGGATGCTCTTAAAGAAGAATCTGAGATAATCTATGACTTTTGCATGTGCAACCCTCCCTT
  TTTTGCCAATCAATTGGAAGCCAAGGGAGTAAACTCACGAAATCCTCGAAGACCTCCGCCTAGTTCTGTT
  AATACAGGAGGCATCACAGAGATCATGGCAGAAGGAGGTGAATTAGAGTTTGTTAAAAGGATCATCCATG
  ACAGTCTACAACTTAAAAAAAGATTAAGATGGTATAGCTGCATGCTGGGAAAGAAATGCAGCCTGGCGCC
  TCTGAAGGAGGAGCTTCGCATACAAGGGGTTCCCAAAGTAACGTACACTGAATTCTGTCAAGGTCGGACA
  ATGAGATGGGCCTTAGCTTGGAGTTTTTATGATGATGTCACAGTACCATCACCACCAAGTAAGCGAAGAA
  AATTAGAGAAACCGAGAAAACCCATAACATTCGTGGTGCTGGCGTCCGTGATGAAGGAATTATCCCTCAA
  AGCATCACCTCTGCGCTCGGAGACGGCGGAAGGCATAGTCGTTGTCACGACATGGATTGAAAAAATTCTC
  ACTGATTTGAAGGTCCAGCATAAACGAGTTCCCTGTGGAAAAGAGGAAGTCAGCCTTTTCCTAACGGCCA
  TAGAAAACTCCTGGATTCATTTAAGGAGAAAGAAAAGAGAGCGTGTGAGACAGCTGAGAGAAGTTCCCCG
  AGCTCCTGAGGACGTCATTCAGGCCTTGGAAGAGAAAAAGCCCACCCCCAAAGAGTCTGGCAATAGCCAA
  GAACTGGCCAGGGGCCCCCAGGAGAGGACCCCCTGTGGGCCTGCTCTGCGGGAAGGCGAGGCTGCCGCTG
  TGGAGGGCCCGTGCCCGAGCCAGGAGTCCCTGTCCCAGGAGGAAAACCCGGAACCCACGGAGGATGAAAG
  GAGTGAGGAAAAGGGAGGGGTGGAGGTTTTGGAAAGTTGTCAAGGCTCTAGCAACGGAGCCCAGGACCAA
  GAGGCTTCTGAGCAGTTCGGCAGCCCAGTGGCTGAAAGGGGGAAACGTCTCCCAGGAGTGGCCGGACAGT
  ACCTGTTTAAGTGTTTGATAAACGTTAAGAAGGAGGTGGACGATGCCTTAGTGGAGATGCACTGGGTTGA
  GGGCCAGAACAGGGATCTGATGAACCAGCTTTGCACCTACATACGTAACCAAATTTTCAGGCTTGTTGCA
  GTTAACTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_024086
• ORF Size: 1689 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_024086.3, NP_076991.3
• RefSeq Size: 5758
• RefSeq ORF: 1689
• Locus ID: 79066
• Gene Summary: RNA N6-methyltransferase that methylates adenosine residues of a subset of RNAs and plays a key role in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts (PubMed:28525753). Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs) (PubMed:28525753). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure (PubMed:28525753). In presence of S-adenosyl-L-methionine, binds the 3'-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, leading to intron retention and preventing MAT2A mRNA splicing (PubMed:28525753). In S-adenosyl-L-methionine-limiting conditions, binds the 3'-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, leading to stimulate splicing of the MAT2A retained intron, and promoting expression of MAT2A (PubMed:28525753). In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA): specifically N6-methylates adenine in position 43 of U6 snRNAs (PubMed:28525753). Specifically binds the 3'-end of the MALAT1 long non-coding RNA (PubMed:27872311). [UniProtKB/Swiss-Prot Function]