MAP3K8 (NM_005204) Human Untagged Clone

CAT#: SC112674

MAP3K8 (untagged)-Human mitogen-activated protein kinase kinase kinase 8 (MAP3K8)


  "NM_005204" in other vectors (7)

Reconstitution Protocol

USD 760.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol MAP3K8
Synonyms AURA2; c-COT; COT; EST; ESTF; MEKK8; Tpl-2; TPL2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_005204 edited
ATGGAGTACATGAGCACTGGAAGTGACAATAAAGAAGAGATTGATTTATTAATTAAACAT
TTAAATGTGTCTGATGTAATAGACATTATGGAAAATCTTTATGCAAGTGAAGAGCCAGCA
GTTTATGAACCCAGTCTAATGACCATGTGTCAAGACAGTAATCAAAACGATGAGCGTTCT
AAGTCTCTGCTGCTTAGTGGCCAAGAGGTACCATGGTTGTCATCAGTCAGATACGGAACT
GTGGAGGATTTGCTTGCTTTTGCAAACCATATATCCAACACTGCAAAGCATTTTTATGGA
CAACGACCACAGGAATCTGGAATTTTATTAAACATGGTCATCACTCCCCAAAATGGACGT
TACCAAATAGATTCCGATGTTCTCCTGATCCCCTGGAAGCTGACTTACAGGAATATTGGT
TCTGATTTTATTCCTCGGGGCGCCTTTGGAAAGGTATACTTGGCACAAGATATAAAGACG
AAGAAAAGAATGGCGTGTAAACTGATCCCAGTAGATCAATTTAAGCCATCTGATGTGGAA
ATCCAGGCTTGCTTCCGGCACGAGAACATCGCAGAGCTGTATGGCGCAGTCCTGTGGGGT
GAAACTGTCCATCTCTTTATGGAAGCAGGCGAGGGAGGGTCTGTTCTGGAGAAACTGGAG
AGCTGTGGACCAATGAGAGAATTTGAAATTATTTGGGTGACAAAGCATGTTCTCAAGGGA
CTTGATTTTCTACACTCAAAGAAAGTGATCCATCATGATATTAAACCTAGCAACATTGTT
TTCATGTCCACAAAAGCTGTTTTGGTGGATTTTGGCCTAAGTGTTCAAATGACCGAAGAT
GTCTATTTTCCTAAGGACCTCCGAGGAACAGAGATTTACATGAGCCCAGAGGTCATCCTG
TGCAGGGGCCATTCAACCAAAGCAGACATCTACAGCCTGGGGGCCACGCTCATCCACATG
CAGACGGGCACCCCACCCTGGGTGAAGCGCTACCCTCGCTCAGCCTATCCCTCCTACCTG
TACATAATCCACAAGCAAGCACCTCCACTGGAAGACATTGCAGATGACTGCAGTCCAGGG
ATGAGAGAGCTGATAGAAGCTTCCCTGGAGAGAAACCCCAATCACCGCCCAAGAGCCGCA
GACCTACTAAAACATGAGGCCCTGAACCCGCCCAGAGAGGATCAGCCACGCTGTCAGAGT
CTGGACTCTGCCCTCTTGGAGCGCAAGAGGCTGCTGAGTAGGAAGGAGCTGGAACTTCCT
GAGAACATTGCTGATTCTTCGTGCACAGGAAGCACCGAGGAATCTGAGATGCTCAAGAGG
CAACGCTCTCTCTACATCGACCTCGGCGCTCTGGCTGGCTACTTCAATCTTGTTCGGGGA
CCACCAACGCTTGAATATGGCTGA
>OriGene 5' read for NM_005204 unedited
AGTCAAATTTGTATAGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCGGGGATGCGG
GGACCCGCTGTCACTGCGCCTCCCGCTGCCGACGCCGCCTGGACGGCCGCACTCTCCCTG
CCCGAGACCCGATGCAATCTTCTTACCGCGAAGAAGCCAGGGGAATAGGTAGCCACATCT
TGTTTGCAGATAAGAAAGGAAGCTAACGCAGTATCTGCAAAGCCAGGAGTCTGACTCAGT
ACTTTTCTCACTCATGCATACAAAGCAGCTAAAAATGACACAGCTTATTTACCATGCCCC
TGACACTGCACTGAGCACTTTATGAGCTTGAACTCTGTTAATCCTCACGACCACCTCATG
AGACTCTCCAGAAAGAGCAACAGTAATGGAGTACATGAGCACTGGAAGTGACAATAAAGA
AGAGATTGATTTATTAATTAAACATTTAAATGTGTCTGATGTAATAGACATTATGGAAAA
TCTTTATGCAAGTGAAGAGCCAGCAGTTTATGAACCCAGTCTAATGACCATGTGTCAAGA
CAGTAATCAAAACGATGAGCGTTCTAAGTCTCTGCTGCTTAGTGGCCAAGAGGTACCATG
GTTGTCATCAGTCAGATACGGAACTGTGGAGGATTTGCTTGCTTTTGCAAACCATATATC
CAACACTGCAAAGCATTTTTATGGACAACGACCACAGGAATCTGGAATTTTATTAAACAT
GGNTCATCACTCCCCANAATGGACGTTACCAAATAGATTCCGATGTTCTNCCTGACCCCT
GNAAGCTGACTTACAGGAATATTGGTTCTGATTTTATCCTCGGGGCGCCTTTGGAAAGGT
ATACTTGNCACAAGANTTANGACGAAGAAAGAATGGCGTGTAAACTGATCCCAGTAGATC
ATTTAAGC
>OriGene 3' read for NM_005204 unedited
NTTTAGCTTGACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGGGAAAATCCGGAATTTTT
TCAGGGGGTTTGCCCACCCCCTTTGGACAAAAGTTTGGGGTTTTTTTGGCATAAGGGAAA
AACATTCTTTACATAAAACTCCCCACATTCTTTACAAGGGTCCAAATTTTTTTTTGTTTA
AAAAATTCTTGAGGGCTTTTGATTTAAAACATCCCCAGTTTGCTTTTTTAATATCCAAAG
GCTTAAAAAAAAATTTATTCTTAAAAATTCCAAAATCTTCAATTTTTTACAAAAGTTTTT
TTTTTTTTTAAGGGGCACATTCTCTTCCTTGGGGAGGAACAAAAATTTTGGGGTTTACCC
AGTTTTTTCCCCTTTAAAAACATCCCGTTTTGAAGGTTTTCACCATAGCAGGGCCAGTAA
AAAAGGTTTCCATAGGAACCCAAAAAAAACTGGCCCTAGGGGCCGGGTGGCAAAGGGCTT
TTAATTCATCAGTTACCCTTTTTTAGGACCCTAGCCCAGTTTTTTGCTCCAATTTTAAAG
GGGTTTTAGCCCCCCAAATTTTAAGGAATTCAATGGTTCCCAAAATTTCCCAATAAGGGC
TCAAAGGGTTTTTTATTTTCCCCCATTTTCCCCAAAACCGTTTTTCTGGAAAAAAGGAAT
TTTCCCTTCTTAAATAAAAACCAAAAAATAGGATTTTGGGCTCCCTCTTTTACCCTGTGG
AAGGGGTATTTTTAAATTTGGGACCAAGGGCCCAGGGAATGAAACAATTCGGAAACCCAA
TCCTTGTGGGAACCTAAAATTCCCGCCTAAAAACCCCGCCCCCTGAAAAAGAGAACCTGG
AAACCTGGGGCTATTAAAAAGCTTCCCTGTTAAACCCCAG
Restriction Sites NotI-NotI     
ACCN NM_005204
Insert Size 2870 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005204.2, NP_005195.2
RefSeq Size 3096 bp
RefSeq ORF 1404 bp
Locus ID 1326
Cytogenetics 10p11.23
Domains pkinase, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways MAPK signaling pathway, T cell receptor signaling pathway, Toll-like receptor signaling pathway
Gene Summary 'This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]'
Transcript Variant: This variant (1) represents the longest transcript. Variants 1-3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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