MAP3K8 (NM_005204) Human Untagged Clone
CAT#: SC112674
MAP3K8 (untagged)-Human mitogen-activated protein kinase kinase kinase 8 (MAP3K8)
"NM_005204" in other vectors (7)
Product Images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Symbol | MAP3K8 |
Synonyms | AURA2; c-COT; COT; EST; ESTF; MEKK8; Tpl-2; TPL2 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF sequence for NM_005204 edited
ATGGAGTACATGAGCACTGGAAGTGACAATAAAGAAGAGATTGATTTATTAATTAAACAT TTAAATGTGTCTGATGTAATAGACATTATGGAAAATCTTTATGCAAGTGAAGAGCCAGCA GTTTATGAACCCAGTCTAATGACCATGTGTCAAGACAGTAATCAAAACGATGAGCGTTCT AAGTCTCTGCTGCTTAGTGGCCAAGAGGTACCATGGTTGTCATCAGTCAGATACGGAACT GTGGAGGATTTGCTTGCTTTTGCAAACCATATATCCAACACTGCAAAGCATTTTTATGGA CAACGACCACAGGAATCTGGAATTTTATTAAACATGGTCATCACTCCCCAAAATGGACGT TACCAAATAGATTCCGATGTTCTCCTGATCCCCTGGAAGCTGACTTACAGGAATATTGGT TCTGATTTTATTCCTCGGGGCGCCTTTGGAAAGGTATACTTGGCACAAGATATAAAGACG AAGAAAAGAATGGCGTGTAAACTGATCCCAGTAGATCAATTTAAGCCATCTGATGTGGAA ATCCAGGCTTGCTTCCGGCACGAGAACATCGCAGAGCTGTATGGCGCAGTCCTGTGGGGT GAAACTGTCCATCTCTTTATGGAAGCAGGCGAGGGAGGGTCTGTTCTGGAGAAACTGGAG AGCTGTGGACCAATGAGAGAATTTGAAATTATTTGGGTGACAAAGCATGTTCTCAAGGGA CTTGATTTTCTACACTCAAAGAAAGTGATCCATCATGATATTAAACCTAGCAACATTGTT TTCATGTCCACAAAAGCTGTTTTGGTGGATTTTGGCCTAAGTGTTCAAATGACCGAAGAT GTCTATTTTCCTAAGGACCTCCGAGGAACAGAGATTTACATGAGCCCAGAGGTCATCCTG TGCAGGGGCCATTCAACCAAAGCAGACATCTACAGCCTGGGGGCCACGCTCATCCACATG CAGACGGGCACCCCACCCTGGGTGAAGCGCTACCCTCGCTCAGCCTATCCCTCCTACCTG TACATAATCCACAAGCAAGCACCTCCACTGGAAGACATTGCAGATGACTGCAGTCCAGGG ATGAGAGAGCTGATAGAAGCTTCCCTGGAGAGAAACCCCAATCACCGCCCAAGAGCCGCA GACCTACTAAAACATGAGGCCCTGAACCCGCCCAGAGAGGATCAGCCACGCTGTCAGAGT CTGGACTCTGCCCTCTTGGAGCGCAAGAGGCTGCTGAGTAGGAAGGAGCTGGAACTTCCT GAGAACATTGCTGATTCTTCGTGCACAGGAAGCACCGAGGAATCTGAGATGCTCAAGAGG CAACGCTCTCTCTACATCGACCTCGGCGCTCTGGCTGGCTACTTCAATCTTGTTCGGGGA CCACCAACGCTTGAATATGGCTGA >OriGene 5' read for NM_005204 unedited
AGTCAAATTTGTATAGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCGGGGATGCGG GGACCCGCTGTCACTGCGCCTCCCGCTGCCGACGCCGCCTGGACGGCCGCACTCTCCCTG CCCGAGACCCGATGCAATCTTCTTACCGCGAAGAAGCCAGGGGAATAGGTAGCCACATCT TGTTTGCAGATAAGAAAGGAAGCTAACGCAGTATCTGCAAAGCCAGGAGTCTGACTCAGT ACTTTTCTCACTCATGCATACAAAGCAGCTAAAAATGACACAGCTTATTTACCATGCCCC TGACACTGCACTGAGCACTTTATGAGCTTGAACTCTGTTAATCCTCACGACCACCTCATG AGACTCTCCAGAAAGAGCAACAGTAATGGAGTACATGAGCACTGGAAGTGACAATAAAGA AGAGATTGATTTATTAATTAAACATTTAAATGTGTCTGATGTAATAGACATTATGGAAAA TCTTTATGCAAGTGAAGAGCCAGCAGTTTATGAACCCAGTCTAATGACCATGTGTCAAGA CAGTAATCAAAACGATGAGCGTTCTAAGTCTCTGCTGCTTAGTGGCCAAGAGGTACCATG GTTGTCATCAGTCAGATACGGAACTGTGGAGGATTTGCTTGCTTTTGCAAACCATATATC CAACACTGCAAAGCATTTTTATGGACAACGACCACAGGAATCTGGAATTTTATTAAACAT GGNTCATCACTCCCCANAATGGACGTTACCAAATAGATTCCGATGTTCTNCCTGACCCCT GNAAGCTGACTTACAGGAATATTGGTTCTGATTTTATCCTCGGGGCGCCTTTGGAAAGGT ATACTTGNCACAAGANTTANGACGAAGAAAGAATGGCGTGTAAACTGATCCCAGTAGATC ATTTAAGC >OriGene 3' read for NM_005204 unedited
NTTTAGCTTGACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGGGAAAATCCGGAATTTTT TCAGGGGGTTTGCCCACCCCCTTTGGACAAAAGTTTGGGGTTTTTTTGGCATAAGGGAAA AACATTCTTTACATAAAACTCCCCACATTCTTTACAAGGGTCCAAATTTTTTTTTGTTTA AAAAATTCTTGAGGGCTTTTGATTTAAAACATCCCCAGTTTGCTTTTTTAATATCCAAAG GCTTAAAAAAAAATTTATTCTTAAAAATTCCAAAATCTTCAATTTTTTACAAAAGTTTTT TTTTTTTTTAAGGGGCACATTCTCTTCCTTGGGGAGGAACAAAAATTTTGGGGTTTACCC AGTTTTTTCCCCTTTAAAAACATCCCGTTTTGAAGGTTTTCACCATAGCAGGGCCAGTAA AAAAGGTTTCCATAGGAACCCAAAAAAAACTGGCCCTAGGGGCCGGGTGGCAAAGGGCTT TTAATTCATCAGTTACCCTTTTTTAGGACCCTAGCCCAGTTTTTTGCTCCAATTTTAAAG GGGTTTTAGCCCCCCAAATTTTAAGGAATTCAATGGTTCCCAAAATTTCCCAATAAGGGC TCAAAGGGTTTTTTATTTTCCCCCATTTTCCCCAAAACCGTTTTTCTGGAAAAAAGGAAT TTTCCCTTCTTAAATAAAAACCAAAAAATAGGATTTTGGGCTCCCTCTTTTACCCTGTGG AAGGGGTATTTTTAAATTTGGGACCAAGGGCCCAGGGAATGAAACAATTCGGAAACCCAA TCCTTGTGGGAACCTAAAATTCCCGCCTAAAAACCCCGCCCCCTGAAAAAGAGAACCTGG AAACCTGGGGCTATTAAAAAGCTTCCCTGTTAAACCCCAG |
Restriction Sites | NotI-NotI |
ACCN | NM_005204 |
Insert Size | 2870 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005204.2, NP_005195.2 |
RefSeq Size | 3096 bp |
RefSeq ORF | 1404 bp |
Locus ID | 1326 |
Cytogenetics | 10p11.23 |
Domains | pkinase, TyrKc, S_TKc |
Protein Families | Druggable Genome, Protein Kinase |
Protein Pathways | MAPK signaling pathway, T cell receptor signaling pathway, Toll-like receptor signaling pathway |
Gene Summary | 'This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]' Transcript Variant: This variant (1) represents the longest transcript. Variants 1-3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC323633 | MAP3K8 (untagged)-Kinase deficient mutant (D253A) of Human mitogen-activated protein kinase kinase kinase 8 (MAP3K8) |
USD 760.00 |
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RC211171 | MAP3K8 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 8 (MAP3K8) |
USD 420.00 |
|
RG211171 | MAP3K8 (GFP-tagged) - Human mitogen-activated protein kinase kinase kinase 8 (MAP3K8) |
USD 460.00 |
|
RC211171L1 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase 8 (MAP3K8), Myc-DDK-tagged |
USD 768.00 |
|
RC211171L2 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase 8 (MAP3K8), mGFP tagged |
USD 620.00 |
|
RC211171L3 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase 8 (MAP3K8), Myc-DDK-tagged |
USD 768.00 |
|
RC211171L4 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase 8 (MAP3K8), mGFP tagged |
USD 620.00 |
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