MAP3K8 (NM_005204) Human Untagged Clone

CAT#: SC323633

MAP3K8 (untagged)-Kinase deficient mutant (D253A) of Human mitogen-activated protein kinase kinase kinase 8 (MAP3K8)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAP3K8
• Synonyms: AURA2; c-COT; COT; EST; ESTF; MEKK8; Tpl-2; TPL2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323633 sequence for NM_005204 edited (data generated by NextGen Sequencing)
  ATGGAGTACATGAGCACTGGAAGTGACAATAAAGAAGAGATTGATTTATTAATTAAACAT
  TTAAATGTGTCTGATGTAATAGACATTATGGAAAATCTTTATGCAAGTGAAGAGCCAGCA
  GTTTATGAACCCAGTCTAATGACCATGTGTCAAGACAGTAATCAAAACGATGAGCGTTCT
  AAGTCTCTGCTGCTTAGTGGCCAAGAGGTACCATGGTTGTCATCAGTCAGATACGGAACT
  GTGGAGGATTTGCTTGCTTTTGCAAACCATATATCCAACACTGCAAAGCATTTTTATGGA
  CAACGACCACAGGAATCTGGAATTTTATTAAACATGGTCATCACTCCCCAAAATGGACGT
  TACCAAATAGATTCCGATGTTCTCCTGATCCCCTGGAAGCTGACTTACAGGAATATTGGT
  TCTGATTTTATTCCTCGGGGCGCCTTTGGAAAGGTATACTTGGCACAAGATATAAAGACG
  AAGAAAAGAATGGCGTGTAAACTGATCCCAGTAGATCAATTTAAGCCATCTGATGTGGAA
  ATCCAGGCTTGCTTCCGGCACGAGAACATCGCAGAGCTGTATGGCGCAGTCCTGTGGGGT
  GAAACTGTCCATCTCTTTATGGAAGCAGGCGAGGGAGGGTCTGTTCTGGAGAAACTGGAG
  AGCTGTGGACCAATGAGAGAATTTGAAATTATTTGGGTGACAAAGCATGTTCTCAAGGGA
  CTTGATTTTCTACACTCAAAGAAAGTGATCCATCATGCCATTAAACCTAGCAACATTGTT
  TTCATGTCCACAAAAGCTGTTTTGGTGGATTTTGGCCTAAGTGTTCAAATGACCGAAGAT
  GTCTATTTTCCTAAGGACCTCCGAGGAACAGAGATTTACATGAGCCAAGAGGTCATCCTG
  TGCAGGGGCCATTCAACCAAAGCAGACATCTACAGCCTGGGGGCCACGCTCATCCACATG
  CAGACGGGCACCCCACCCTGGGTGAAGCGCTACCCTCGCTCAGCCTATCCCTCCTACCTG
  TACATAATCCACAAGCAAGCACCTCCACTGGAAGACATTGCAGATGACTGCAGTCCAGGG
  ATGAGAGAGCTGATAGAAGCTTCCCTGGAGAGAAACCCCAATCACCGCCCAAGAGCCGCA
  GACCTACTAAAACATGAGGCCCTGAACCCGCCGAGAGAGGATCAGCCACGCTGTCAGAGT
  CTGGACTCTGCCCTCTTGGAGCGCAAGAGGCTGCTGAGTAGGAAGGAGCTGGAACTTCCT
  GAGAACATTGCTGATTCTTCGTGCACAGGAAGCACCGAGGAATCTGAGATGCTCAAGAGG
  CAACGCTCTCTCTACATCGACCTCGGCGCTCTGGCTGGCTACTTCAATCTTGTTCGGGGA
  CCACCAACGCTTGAATATGGCTGA
  
  Clone variation with respect to NM_005204.3
  234 t=>c;758 a=>c;759 t=>c;887 c=>a;1173 c=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_005204 unedited
  CCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGGGAAGCGGGGA
  CCCGCTGTCACTGCGCCTCCCGCTGCCGACGCCGCCTGGACGGCCGCACTCTCCCTGCCCGAGACCCGAT
  GCAATCTTCTTACCGCGAAGAAGCCAGGGGAATAGGTAGCCACATCTTGTTTGCAGATAAGAAAGGAAGC
  TAACGCAGTATCTGCAAAGCCAGGAGTCTGACTCAGTACTTTTTCTCACTCATGCATACAAAGCAGCTAA
  GAAATGACACAGCTTTATTTTACCATGCCCCCTTGACACTGGCACTGGAGCACTTTTATGAGCCTGGACC
  TCGGGTTAATTCCTCCACAACCCCCCTCAGGAAAACTCCCCAGGAAAAAAGCACCAGGTATGGGAATACA
  CATAGCACACTGAAGTTTGAAAAATAAGAAAAGAAAATGGATTTATTTAATTAAAACATTAAAAGTGGTG
  TTGAGAGGTAATAAAACTTTAGGGAAAAATCTTTTGGCCGTGTAAAGACCCGCCATTTTAAAGACCCCAG
  TCAAAGAGCATAGTGGTCAAGACGGTACCAACCGGATGGAGCTTTTTAGCTCCTGCTGGGGATTGGGGCC
  AAAAAGGCCCCGGGGGTTGGTAACATTTAAATACCAGCACTGGGGGGGGTTTTGTGTTTTTTTTTGACAC
  CCTATTCCCCAACCGGTGAAAATTTTTTTTGGGGGCCCACCCCCCGGGGCTTGGGGTATTTTTATAACGT
  GGGGCCCTCCCCCCCCAGGAGGGGTCTTCACAAAATACCTACGGTGTCCTCGTACACCCGCGAGCAGACT
  TTCTAGAGAGATATGTGTGCACTATTCATCGCGCGCGCCTCGTGAGATGTCTGTCTGCGCATATAGCAGA
  CAAAAGCGTGTCAGATCGCTCCATCAGTGATCACTGCTGCATGATGAGCAGCGCGTGCG
  
  Kinase domain sequence

  >SC323633 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  GWCTGGACTGMTTAAGGATATTGGTTCTGATTTTATTCCTCGGGGCGCCTTTGGAAAGGTATACTTGGCA
  CAAGATATAAAGACGAAGAAAAGAATGGCGTGTAAACTGATCCCAGTAGATCAATTTAAGCCATCTGATG
  TGGAAATCCAGGCTTGCTTCCGGCACGAGAACATCGCAGAGCTGTATGGCGCAGTCCTGTGGGGTGAAAC
  TGTCCATCTCTTTATGGAAGCAGGCGAGGGAGGGTCTGTTCTGGA
  
• Restriction Sites: Please inquire
• ACCN: NM_005204
• Insert Size: 2870 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005204.2, NP_005195.2
• RefSeq Size: 3096 bp
• RefSeq ORF: 1404 bp
• Locus ID: 1326
• Cytogenetics: 10p11.23
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway, T cell receptor signaling pathway, Toll-like receptor signaling pathway
• Gene Summary: 'This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]'
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1-3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.