Selenophosphate synthetase 2 (SEPHS2) (NM_012248) Human Untagged Clone

CAT#: SC115458

SEPHS2 (untagged)-Human selenophosphate synthetase 2 (SEPHS2) (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)

Specifications

Product Data

• Type: Human Untagged Clone
• Symbol: SEPHS2
• Synonyms: SPS2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115458 sequence for NM_012248 edited (data generated by NextGen Sequencing)
  ATGGCGGAAGCCTCGGCGACGGGCGCCTGCGGAGAGGCGATGGCAGCGGCGGAAGGCTCC
  TCGGGCCCGGCGGGCTTGACTCTGGGCCGGAGCTTCTCGAACTACCGGCCCTTCGAGCCC
  CAGGCGTTGGGCCTCAGCCCGAGCTGGCGGCTGACGGGCTTCTCCGGCATGAAGGGCTGA
  GGCTGCAAGGTCCCGCAGGAGGCGCTGCTCAAACTCCTGGCGGGACTGACGCGGCCGGAC
  GTGCGGCCCCCGCTGGGCCGGGGCCTGGTGGGTGGCCAGGAAGAGGCGTCCCAGGAAGCC
  GGCCTGCCGGCAGGAGCGGGCCCCAGCCCCACCTTTCCAGCCCTGGGCATCGGGATGGAC
  TCCTGCGTCATCCCCCTGAGGCACGGGGGCCTGTCACTGGTGCAGACCACGGACTTCTTT
  TACCCCTTGGTAGAAGATCCCTACATGATGGGGCGCATAGCTTGTGCCAACGTGCTGAGT
  GACCTCTACGCCATGGGGATTACTGAGTGTGACAACATGTTGATGTTACTCAGCGTCAGC
  CAGAGTATGAGTGAGGAGGAACGCGAAAAGGTAACGCCACTCATGGTCAAAGGCTTTCGG
  GATGCGGCTGAGGAAGGAGGGACGGCAGTGACCGGTGGGCAAACGGTGGTCAACCCTTGG
  ATTATAATCGGTGGAGTTGCCACTGTAGTATGCCAACCAAATGAGTTCATAATGCCGGAC
  AGCGCCGTCGTTGGGGACGTGCTGGTGTTAACCAAACCGTTAGGAACCCAGGTTGCTGTC
  AATGCCCACCAATGGCTGGATAATCCTGAAAGATGGAATAAAGTAAAGATGGTGGTCTCC
  AGAGAAGAGGTGGAGCTGGCCTATCAGGAAGCCATGTTCAATATGGCTACCCTCAACAGA
  ACTGCTGCAGGTTTAATGCACACATTTAATGCCCATGCGGCCACAGATATCACAGGCTTT
  GGCATTCTAGGACACTCCCAGAACCTTGCAAAACAACAAAGAAATGAAGTGTCCTTTGTT
  ATTCATAATCTGCCAATAATTGCCAAGATGGCTGCCGTCAGCAAGGCCAGTGGACGGTTT
  GGGCTTCTTCAAGGAACCTCAGCTGAAACCTCTGGGGGATTACTGATTTGTCTGCCAAGA
  GAACAGGCGGCTCGCTTTTGTTCTGAAATCAAATCCTCCAAGTACGGAGAGGGTCACCAA
  GCGTGGATCGTTGGCATTGTGGAAAAGGGAAACCGAACGGCCCGGATCATTGACAAGCCG
  CGAGTTATTGAAGTCCTGCCTCGTGGGGCCACAGCTGCTGTTCTTGCTCCTGACAGTTCA
  AATGCCTCCTCTGAGCCTAGCTCGTGA
  
  Clone variation with respect to NM_012248.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012248 unedited
  AATTTGGTAAACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGTGACGGCTTGAG
  TAGCGCTAGGGAGAATCCCTGCAGGTAATATTTGACTTTTGCTTCATATTAATCTGAGTG
  GAAAATAAAAGGGCCCTCTTCTCCTCTCGCTTCCCTGCCGGGCAGGCGCCATGGCGGAAG
  CCTCGGCGACGGGCGCCTGCGGAGAGGCGATGGCAGCGGCGGAAGGCTCCTCGGGCCCGG
  CGGGCTTGACTCTGGGCCGGAGCTTCTCGAACTACCGGCCCTTCGAGCCCCAGGCGTTGG
  GCCTCAGCCCGAGCTGGCGGCTGACGGGCTTCTCCGGCATGAAGGGCTGAGGCTGCAAGG
  TCCCGCAGGAGGCGCTGCTCAAACTCCTGGCGGGACTGACGCGGCCGGACGTGCGGCCCC
  CGCTGGGCCGGGGCCTGGTGGGTGGCCAGGAAGAGGCGTCCCAGGAAGCCGGCCTGCCGG
  CAGGAGCGGGCCCCAGCCCCACCTTTCCAGCCCTGGGCATCGGGATGGACTCCTGCGTCA
  TCCCCCTGAGGCACGGGGGCCTGTCACTGGTGCAGACCACGGACTTCTTTTACCCCTTGG
  TAGAAGATCCCTACATGATGGGGCGCATAGCTTGTGCCAACGTGCTGAGTGACCTCTACG
  CCATGGGGATTACTGAGTGTGACAACATGTTGATGTTACTCAGCGTCAGCCAGAGTATGA
  GTGAGGAGGAACGCGAAAAGTAACGCCACTCATGGTCAAAGGCTNTCGGAATGCGGCTGA
  NGAAGAGGNACGGCAGTGACCGGTGGGCCAACGGTGGTCAACCCTTGGATTATAATCGGT
  GGAGTTGCCACTGTAGTATGCCAACCAAATGAGTNCATAATGCCGNACAGCGCCGTCGTT
  GGGGACCTGCCTGGGTTAAACCAACCGTAAGGACCCACGTTGCTGTTCATGCCCACCAAT
  GGCTGGAAATCCTGGAGNAGGATAAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012248 unedited
  TAGCTATGGACCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTAGTTTTTCAACAAGGTTTCTTTTATTTATTTAGCAAGAAATTACTCTTTATTGAT
  CAAATCACCAGGAATCTGCCGCAAAAGACTTAACATCTTAATGACCACTGAGGCCTGGGC
  TCTGAGGCTTGCTCTGCCTCTCCCAGTCCTTAATGGGGTTTAGAGGGAGAGACGTCATTA
  ACAGAGACCAAGTCAGATGGTTGCAGGTCCTCCTTTCCCAGTCATCAGACAAGTTGCTCA
  ATTCTTGTCCAGAGCAGGAAAGAGCCAGTACTTCAGTAAAATTGAATAAAGAATTTGATG
  TTGCATTATAAGATCTCTCTTAACAATTTAAATTTATCAACAATATTGGTCCAAAACTCA
  TTTGCTTCTAATTTTTTTCATTTTGCAATTTACTTTGGGTGTGTGTTACTTTAATAATCT
  CACTCCCTGTGCAGAAATTAATCAACCCCGTGCAAAGAAAAATCCCTCAACCTGGAATAG
  CTTCATCTTACTTTGGCAAGAGAAAACAGAGGTATTTTTGCTCCTCTTCTACCCAAGTGA
  TGAACAAATATCCCCCCAAAGTTCAAAATGTTACTCTGGCCTCGGAATGCATATAAAGAC
  TGCTTTAGATGGGCTGATTCCAATCACCTAGAAAGGGCAGCCGGAACCACTATGCAGGCA
  GCCTTCTTTGGAAATTTCTTACATCAACTCTTGAGACCATCCGTGATTGTGGACAATGGC
  TCTAGGTTCAAACAACTTCTGTTCTTTCATCTCACGAGCTAGGCTCAGAAGAAGCATTTT
  GACTGTCAGGAGCAAGAACAGCAGCTGTGGGCCCACGAGCCAGGACTTCCATACTCGCGG
  CTTGCAATGATCCGGCCCGTCGGTTCCCTTTTCACATGCCACGATCACG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012248
• Insert Size: 2240
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.
• OTI Annotation: This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.

Reference Data

• RefSeq: NM_012248.2, NP_036380.2
• RefSeq Size: 2291
• Locus ID: 22928
• Domains: AIRS
• Protein Pathways: Metabolic pathways, Selenoamino acid metabolism
• Gene Summary: This gene encodes an enzyme that catalyzes the production of monoselenophosphate (MSP) from selenide and ATP. MSP is the selenium donor required for synthesis of selenocysteine (Sec), which is co-translationally incorporated into selenoproteins at in-frame UGA codons that normally signal translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein is itself a selenoprotein containing a Sec residue at its active site, suggesting the existence of an autoregulatory mechanism. It is preferentially expressed in tissues implicated in the synthesis of selenoproteins and in sites of blood cell development. A pseudogene for this locus has been identified on chromosome 5. [provided by RefSeq, May 2017]