HNMT (NM_006895) Human Untagged Clone

CAT#: SC115807

HNMT (untagged)-Human histamine N-methyltransferase (HNMT), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HNMT
• Synonyms: HMT; HNMT-S1; HNMT-S2; MRT51
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115807 sequence for NM_006895 edited (data generated by NextGen Sequencing)
  ATGGCATCTTCCATGAGGAGCTTGTTTTCTGACCACGGGAAATATGTTGAATCTTTCCGG
  AGGTTTCTCAACCATTCCACGGAACACCAGTGCATGCAGGAATTCATGGACAAGAAGCTG
  CCAGGCATAATAGGAAGGATTGGAGACACAAAATCAGAAATTAAGATTCTAAGCATAGGC
  GGAGGTGCAGGTGAAATTGATCTTCAAATTCTCTCCAAAGTTCAGGCTCAATACCCAGGA
  GTTTGTATCAACAATGAAGTTGTTGAGCCAAGTGCTGAACAAATTGCCAAATACAAAGAG
  CTTGTAGCCAAGACATCGAACCTCGAGAACGTAAAGTTTGCTTGGCATAAGGAGACATCA
  TCTGAATACCAAAGTAGAATGTTGGAGAAAAAGGAGCTTCAAAAGTGGGACTTTATTCAT
  ATGATTCAAATGCTGTATTATGTAAAAGACATCCCAGCTACCCTGAAATTCTTCCATAGT
  CTCTTAGGTACCAATGCTAAGATGCTCATTATTGTTGTGTCAGGAAGCAGTGGCTGGGAC
  AAGCTGTGGAAAAAGTACGGATCACGCTTTCCCCAGGATGACCTCTGCCAGTATATCACA
  TCAGATGACCTCACTCAGATGCTGGACAACCTAGGGCTTAAGTATGAGTGCTATGACCTT
  TTGTCCACCATGGATATATCTGACTGCTTTATTGATGGTAATGAAAATGGAGACCTGCTT
  TGGGATTTTTTGACTGAAACCTGCAACTTTAATGCCACAGCACCACCTGATCTCAGAGCA
  GAGCTTGGGAAAGATCTACAAGAGCCTGAATTTAGTGCTAAGAAAGAGGGGAAGGTTCTT
  TTTAATAATACTCTGAGTTTCATAGTGATTGAGGCATAA
  
  Clone variation with respect to NM_006895.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006895 unedited
  AGCATTTTGTAATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGGGAAAAAAA
  GAGGGAACTGGNGAAAAGAAGGAGGGGAACTTAAACCTTGCTTCCTGCTCTGTCTTTCTC
  AGAAAACCAAATATGGCATCTTCCATGAGGAGCTTGTTTTCTGACCACGGGAAATATGTT
  GAATCTTTCCGGAGGTTTCTCAACCATTCCACGGAACACCAGTGCATGCAGGAATTCATG
  GACAAGAAGCTGCCAGGCATAATAGGAAGGATTGGAGACACAAAATCAGAAATTAAGATT
  CTAAGCATAGGCGGAGGTGCAGGTGAAATTGATCTTCAAATTCTCTCCAAAGTTCAGGCT
  CAATACCCAGGAGTTTGTATCAACAATGAAGTTGTTGAGCCAAGTGCTGAACAAATTGCC
  AAATACAAACAGCTTGTAGCCAAGACATCGAACCTCGAGAACGTAAAGTTTGCTTGGCAT
  AAGGAGACATCATCTGAATACCAAAGTAGAATGTTGGAGAAAAAGGAGCTTCAAAAGTGG
  GACTTTATTCATATGATTCAAATGCTGTATTATGTAAAAGACATACCAGCTACCCTGAAA
  TTCTTCCATAGTCTCTTAGGTACCAATGCTAAGATGCTCATTATTGTTGTGTCAGGAAGC
  AGTGGCTGGGACAAGCTGTGGAAAAAGTACGGATCACGCTNTCCCCAGGATGACCTCTGC
  CAGTATATCACATCAGATGACCTCACTCAGATGCTGGACAAACCTAGGCTTAAGTATGAG
  TGCTATGACCTTTTGTCCACCATGGATATATCTGACTGGCTTATTGATGGGTATGAAAAT
  GGAGACCTGCTNNTGGATTTTTTGACTGAAACCCTGCACCTTATGCCACACACCACCTGA
  TCTCAGAGCAGAGCTTGGGGAAGAATCTACAGACCTGGATTTATGCTANGAAGAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006895 unedited
  GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACAC
  TGGCAACACATAACTTTATTGGGTTTCTTGAGCTCTGTTTATAATAATAATATGAACACA
  CAAGGTATAAAATAAATGTTTGAAAAGGAAATAATTCATTGATCCCGCTTTACTTAACAT
  TTACCATATGCAAGGCACCATGTTAAACACTGAGAGATCCACAACAATACATAAAACAAC
  ATTCCAAATTCATGCTGAGCACTTTTTTCTGAAACACAAAAACAAATCTGAAAAGTTAGT
  ATGTGACTGTCCCAAATTTTGGTATTATCATACAGTGCAGGAAAAAAACAGGGATAGGTT
  TATCCCTTGAATTTATACAACTTCCCATTGCTGACTAATAGGTTTTCATGGGAATTTCTC
  TCTTTAAAAAGGCTTATGTGTTTCAATTGCACTAAAATTAAGCAGGTGAAAATTTATTTA
  TCCTTGTATACTAAGGGTTTAATAAGGACGAGAAAAAGGCCATATTTACAAACACCAATC
  TATGGACTGCAATTCTTTTGTATCTTTCAATAGCATTGNTAGTTCTAATTTTGTTTGTTT
  TTGCACGTGTGCTTTATACAAAAGACTCTGTAAAGTCTTGATTTCCATACTTCTGGTGAA
  GCTCTTCAAGGTCTTCATGGCCCCATACCCATCAATCATGTGTGCCTCACAGATTTCCTT
  TGTCAGTAGTACATATCAGTATGATTAACGGGTTCAGTGTTCATTTTCAGGAATCTGATT
  GGATAACTGTCGTTNCATTTGNNTGAACATATAANTCAAAAGCATTGATGAAGTTGTCCA
  AAAAGAGAGCAATGCAGTCCTTTTACTTATTATGACAACAAGCTNGTAGTTNATTANGAA
  ATAAGCTCTCTATGCAGGNATTCCCTGGGGAAAACTAGTGATGGAAAAGGGTCGTTACTT
  TTTAAGNGATATACGNACTTCTTGGAAGCCTTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006895
• Insert Size: 6310 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006895.2, NP_008826.1
• RefSeq Size: 3373 bp
• RefSeq ORF: 879 bp
• Locus ID: 3176
• Cytogenetics: 2q22.1
• Protein Families: Druggable Genome
• Protein Pathways: Histidine metabolism
• Gene Summary: 'In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.