HNMT (NM_006895) Human Untagged Clone

CAT#: SC321115

HNMT (untagged)-Human histamine N-methyltransferase (HNMT), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HNMT
• Synonyms: HMT; HNMT-S1; HNMT-S2; MRT51
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006895.2 GAGAAAACCAAATATGGCATCTTCCATGAGGAGCTTGTTTTCTGACCACGGGAAATATGT
  TGAATCTTTCCGGAGGTTTCTCAACCATTCCACGGAACACCAGTGCATGCAGGAATTCAT
  GGACAAGAAGCTGCCAGGCATAATAGGAAGGATTGGAGACACAAAATCAGAAATTAAGAT
  TCTAAGCATAGGCGGAGGTGCAGGTGAAATTGATCTTCAAATTCTCTCCAAAGTTCAGGC
  TCAATACCCAGGAGTTTGTATCAACAATGAAGTTGTTGAGCCAAGTGCTGAACAAATTGC
  CAAATACAAAGAGCTTGTAGCCAAGACATCGAACCTCGAGAACGTAAAGTTTGCTTGGCA
  TAAGGAGACATCATCTGAATACCAAAGTAGAATGTTGGAGAAAAAGGAGCTTCAAAAGTG
  GGACTTTATTCATATGATTCAAATGCTGTATTATGTAAAAGACATCCCAGCTACCCTGAA
  ATTCTTCCATAGTCTCTTAGGTACCAATGCTAAGATGCTCATTATTGTTGTGTCAGGAAG
  CAGTGGCTGGGACAAGCTGTGGAAAAAGTACGGATCACGCTTTCCCCAGGATGACCTCTG
  CCAGTATATCACATCAGATGACCTCACTCAGATGCTGGACAACCTAGGGCTTAAGTATGA
  GTGCTATGACCTTTTGTCCACCATGGATATATCTGACTGCTTTATTGATGGTGATGAAAA
  TGGAGACCTGCTTTGGGATTTTTTGACTGAAACCTGCAACTTTAATGCCACAGCACCACC
  TGATCTCAGAGCAGAGCTTGGGAAAGATCTACAAGAGCCTGAATTTAGTGCTAAGAAAGA
  GGGGAAGGTTCTTTTTAATAATACTCTGAGTTTCATAGTGATTGAGGCATAACTATCAAT
  CACAAAAGTATATTCAAAAATTATATTTTGAACAACTCGAATCACTCATTTATTTCCATA
  TTAAAATCACAAACTCATCCATTAATGTAGATAAAGCACTGTTTGGATATGAGATGTAGC
  AAATTCCAATACATTATTGGACTTCCATTTGGAATCATATGGGATACTGCTGGTCTTATC
  CTGTCCCTCCTCCAGGTAGAGAGACCACAAGCAGGCTCAACATAACATAAGCTAGAAAAA
  TTAGATGACTGAATTTCTATGGCATATTGATAATAAAATTCATTCCATTTGCTGATTGTC
  TGAAATTTTCTAGAATACTAATAAAATACATACTATAGATTCTTTATTAGTGAAGTATGC
  ACTAATCAATACTTTGAACACAAAGCCTGTGTTACTGATTTGGCCGTTTTGTGAAGAAAC
  ATTTATCTTTGTACGTTCTTCTATTGTGCTTTCTATCTAATTTTTATTAATTTGTAAGAG
  TAAGCACCTTTAGAATATTAAAAATTAATTCTCCAAAAAAAAAAAAAAAAAAAAAAAAAA
  AA
• Restriction Sites: Please inquire
• ACCN: NM_006895
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006895.2, NP_008826.1
• RefSeq Size: 3373 bp
• RefSeq ORF: 879 bp
• Locus ID: 3176
• Cytogenetics: 2q22.1
• Protein Families: Druggable Genome
• Protein Pathways: Histidine metabolism
• Gene Summary: 'In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.