emopamil binding protein (EBP) (NM_006579) Human Untagged Clone

CAT#: SC116006

EBP (untagged)-Human emopamil binding protein (sterol isomerase) (EBP)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EBP
• Synonyms: CDPX2; CHO2; CPX; CPXD; MEND
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116006 sequence for NM_006579 edited (data generated by NextGen Sequencing)
  ATGACTACCAACGCGGGCCCCTTGCACCCATACTGGCCTCAGCACCTAAGACTGGACAAC
  TTTGTACCTAATGACCGCCCCACCTGGCATATACTGGCTGGCCTCTTCTCTGTCACAGGG
  GTCTTAGTCGTGACCACATGGCTGTTGTCAGGTCGTGCTGCGGTTGTCCCATTGGGGACT
  TGGCGGCGACTGTCCCTGTGCTGGTTTGCAGTGTGTGGGTTCATTCACCTGGTGATCGAG
  GGCTGGTTCGTTCTCTACTACGAAGACCTGCTTGGAGACCAAGCCTTCTTATCTCAACTC
  TGGAAAGAGTATGCCAAGGGAGACAGCCGATACATCCTGGGTGACAACTTCACAGTGTGC
  ATGGAAACCATCACAGCTTGCCTGTGGGGACCACTCAGCCTGTGGGTGGTGATCGCCTTT
  CTCCGCCAGCATCCCCTCCGCTTCATTCTACAGCTTGTGGTCTCTGTGGGCCAGATCTAT
  GGGGATGTGCTCTACTTCCTGACAGAGCACCGCGACGGATTCCAGCACGGAGAGCTGGGC
  CACCCTCTCTACTTCTGGTTTTACTTTGTCTTCATGAATGCCCTGTGGCTGGTGCTGCCT
  GGAGTCCTTGTGCTTGATGCTGTGAAGCACCTCACTCATGCCCAGAGCACGCTGGATGCC
  AAGGCCACAAAAGCCAAGAGCAAGAAGAACTGA
  
  Clone variation with respect to NM_006579.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006579 unedited
  AGGAATTTGTAAACGACTCACTATAGGCGGCCCGCGAATCGGCACGAGGCCTCGTGCCGA
  ATTCGGCACGAGGGACCGGCTTTGTGTTCCGTTCTAGCGCTGCACGCCAGACACCGGCCT
  TTCAATATCCGTCTCTTCCCTGCAGGTTGACGGCGTTGCACGCTCTCGCGGGGAGGCTCT
  GGCTTTCCAAACGCTGGCACCGAGGGTTNTTCTGTTCCTTTTTTTTTTTTTTTTTTAACT
  TCCTGCCTATACACACGCAGCCATCAGCCCACAAAGACATGACTACCAACGCGGGCCCCT
  TGCACCCATACTGGCCTCAGCACCTAAGACTGGACAACTTTGTACCTAATGACCGCCCCA
  CCTGGCATATACTGGCTGGCCTCTTCTCTGTCACAGGGGTCTTAGTCGTGACCACATGGC
  TGTTGTCAGGTCGTGCTGCGGTTGTCCCATTGGGGACTTGGCGGCGACTGTCCCTGTGCT
  GGTTTGCAGTGTGTGGGTTCATTCACCTGGTGATCGAGGGCTGGTTCGTTCTCTACTACG
  AAGACCTGCTTGGAGACCAAGCCTTCTTATCTCAACTCTGGAAAGAGTATGCCAAGGGAG
  ACAGCCGATACATCCTGGGTGACAACTTCACAGTGTGCATGGAAACCATCACAGCTTGCC
  TGTGGGGACCACTCAGCCTGTGGGTGGTGATCGCCTTTCTCCGCCAGCATCCCCTCCGCT
  TTATTCTACAGCTTGTGGTCTCTGTGGGCCAGATCTATGGGGGATGTGCTCTACTTCCTG
  ACAGACACCGCGACGGATCCCGCACGGAGAGCTGGGCCCCCCTCCTACTTCGGGTTTACT
  TTGCCTCATGAAGCCCTGGGGCTGAGCTGCCTGAAACCCCTGGCTTGAGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006579 unedited
  TGTAACGAGGCTCGCATTTTAGNATCGAGCCCTCCCCCCCTCCTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTAAAGGCAAAATCTTTTATTTCCTGATTTTAAAAATCGTC
  ACCATCATAAACCTCCTGGCCTTTCTTGCACCCGGGTTCTAATGGCTCCCAACAGGGCCT
  TCACACAGCCCTTTTATTCCCCTTTTTGGGCCTGCCAATGACCCTGACGGTGAACCACCA
  AACAGCTTCGCCCCTCCAAACTGGGGGAGCCCGACCACCATTTTTTGGCAGGCAAATAGC
  TTTTCCTGGGCCAGGGTTCCACCCTGGTCCACCATTCCCAAACCCTTTTTGCCCCGGGCT
  TCCGGGGCCTTGGCATCCAACGGGCTTTGGGCATGAGGGAGGCTTTTCCTATTATCAAGT
  ATAAGGACTTCAGGGAGAACCACCCCCAGGGCCCTTCTTACCACCCCCTTTTACCCCCCG
  TTCCGAGGGGGGCCACACTTCCCGCGCTGGAATCCCCCGCGGGGCTCTGGCTGCAATTCA
  AACCCCTCCCCTTACCACCCGCCCCCCCAGAACCATAATCTCACAAAGAATCTTCCGCCA
  TCTTCGCCCCCACAAGGTCCCCTCCACCCCACAGGCGACGGTTCCTTCCCAGTTTTTTTT
  CTTGGTTTCCCCGCCCATTTGTTCCCGTTTTCCCCAGCACGGATGCGCCGTCCCCCTGCA
  ATACCTTTTGAAGATTCTTATACCCCCCTTGTTTTCCACCCCCCTCCCCCGCACCCAAGA
  ACCATCTTTTCATCCAGTGCATGAAACCAAATTTTGGAAACACCCCCGCCCCGCCCGCGC
  CATCCCCCCCGGCAACGCCCAAATTTTTGTCCCCCCCTGTTGTCACTATTAACCCCCTTG
  CCACAAAAAACGCTTCCCTATTTTTCCGGCCGCCGGTCCTCCGTCCATATATTTCTCCTT
  CACCCCGCCGCCCACCGCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006579
• ORF Size: 693 bp
• Insert Size: 1250
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_006579.1, NP_006570.1
• RefSeq Size: 1073
• RefSeq ORF: 693
• Locus ID: 10682
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, Steroid biosynthesis
• Gene Summary: The protein encoded by this gene is an integral membrane protein of the endoplasmic reticulum. It is a high affinity binding protein for the antiischemic phenylalkylamine Ca2+ antagonist [3H]emopamil and the photoaffinity label [3H]azidopamil. It is similar to sigma receptors and may be a member of a superfamily of high affinity drug-binding proteins in the endoplasmic reticulum of different tissues. This protein shares structural features with bacterial and eukaryontic drug transporting proteins. It has four putative transmembrane segments and contains two conserved glutamate residues which may be involved in the transport of cationic amphiphilics. Another prominent feature of this protein is its high content of aromatic amino acid residues (>23%) in its transmembrane segments. These aromatic amino acid residues have been suggested to be involved in the drug transport by the P-glycoprotein. Mutations in this gene cause Chondrodysplasia punctata 2 (CDPX2; also known as Conradi-Hunermann syndrome). [provided by RefSeq, Jul 2008]