PPP2R5D (NM_006245) Human Untagged Clone

CAT#: SC116241

PPP2R5D (untagged)-Human protein phosphatase 2, regulatory subunit B', delta (PPP2R5D), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP2R5D
• Synonyms: B56D; B56delta; MRD35
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116241 sequence for NM_006245 edited (data generated by NextGen Sequencing)
  ATGCCCTATAAACTGAAAAAGGAGAAGGAGCCCCCCAAGGTTGCCAAATGCACAGCCAAG
  CCTAGCAGCTCGGGCAAGGATGGTGGAGGCGAGAACACTGAGGAGGCCCAGCCGCAGCCC
  CAGCCCCAGCCCCAGCCCCAAGCCCAGTCTCAGCCACCGTCATCCAACAAGCGTCCCAGC
  AATAGCACGCCGCCCCCCACGCAGCTCAGCAAAATCAAGTACTCAGGGGGGCCCCAGATT
  GTCAAGAAGGAGCGACGGCAAAGCTCCTCCCGCTTCAACCTCAGCAAGAATCGGGAGCTG
  CAGAAGCTTCCTGCCCTGAAAGATTCGCCAACCCAGGAGCGGGAGGAGCTGTTTATCCAG
  AAGCTACGCCAGTGCTGTGTCCTCTTTGACTTCGTGTCAGACCCACTCAGTGACCTCAAA
  TTCAAGGAGGTGAAGCGGGCAGGACTCAACGAGATGGTGGAGTACATCACCCATAGCCGT
  GATGTTGTCACTGAGGCCATTTACCCTGAGGCTGTCACCATGTTTTCAGTGAACCTCTTC
  CGGACGCTGCCACCTTCATCGAATCCCACAGGGGCTGAGTTTGACCCAGAGGAAGATGAG
  CCCACCCTGGAAGCTGCTTGGCCACATCTCCAGCTCGTGTATGAGTTCTTCTTACGTTTC
  CTTGAGTCTCCTGATTTCCAGCCAAACATAGCCAAGAAGTACATCGACCAGAAGTTTGTA
  CTTGCTCTCCTAGACCTATTTGACAGTGAGGATCCTCGAGAGCGGGACTTCCTCAAGACC
  ATTTTGCATCGCATCTATGGCAAGTTTTTGGGGCTCCGGGCTTATATCCGTAGGCAGATC
  AACCACATCTTCTACAGGTTCATCTACGAGACGGAGCATCACAACGGGATTGCTGAGCTC
  CTGGAGATCCTGGGCAGCATCATCAATGGCTTTGCCCTGCCCCTTAAAGAAGAGCACAAG
  ATGTTCCTCATCCGTGTCCTACTTCCCCTTCACAAGGTCAAGTCCCTGAGTGTCTACCAC
  CCTCAGCTGGCATACTGTGTGGTACAATTCCTGGAGAAGGAGAGCAGTCTGACTGAGCCG
  GTAATTGTGGGACTTCTCAAGTTTTGGCCCAAGACCCACAGCCCCAAGGAGGTGATGTTC
  TTGAATGAGCTGGAGGAGATTCTGGACGTCATTGAACCTTCTGAGTTCAGCAAAGTGATG
  GAACCCCTCTTCCGCCAGCTGGCCAAGTGTGTCTCTAGCCCCCATTTCCAGGTGGCAGAG
  CGTGCTCTCTATTACTGGAACAATGAGTACATCATGAGCCTGATAAGTGACAATGCTGCC
  CGAGTCCTCCCCATCATGTTCCCTGCACTCTACAGGAACTCCAAGAGCCACTGGAACAAG
  ACAATCCATGGACTGATCTATAATGCCCTGAAGTTGTTTATGGAAATGAATCAGAAGCTG
  TTTGATGACTGCACACAACAATACAAGGCAGAGAAGCAGAAGGGCCGGTTCCGAATGAAG
  GAAAGGGAAGAGATGTGGCAAAAAATCGAGGAGCTGGCCCGGCTTAATCCCCAGTATCCC
  ATGTTCCGAGCCCCTCCACCACTGCCCCCTGTGTACTCGATGGAGACAGAGACCCCCACA
  GCTGAGGACATCCAGCTTCTGAAGAGGACTGTGGAGACTGAGGCTGTTCAGATGCTAAAA
  GACATCAAGAAGGAGAAAGTGCTGCTGCGGAGGAAGTCGGAGCTGCCCCAGGACGTGTAC
  ACCATCAAGGCACTGGAGGCGCACAAGCGGGCGGAAGAGTTCCTAACTGCCAGCCAGGAG
  GCTCTCTGA
  
  Clone variation with respect to NM_006245.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006245 unedited
  ATTATGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCTCGACCCGGG
  CGCAGCGCGCAGGCGGTGGCGAAGAGACGCCGAGCGGGCCGAGTGCGGCCGAGCAAAGCC
  GGAGCCGGAGCGGGGCCGCAGGAGACGGGCCGGGTCCGGACGGGCCGAGATGCCCTATAA
  ACTGAAAAAGGAGAAGGAGCCCCCCAAGGTTGCCAAATGCACAGCCAAGCCTAGCAGCTC
  GGGCAAGGATGGTGGAGGCGAGAACACTGAGGAGGCCCAGCCGCAGCCCCAGCCCCAGCC
  CCAGCCCCAAGCCCAGTCTCAGCCACCGTCATCCAACAAGCGTCCCAGCAATAGCACGCC
  GCCCCCCACGCAGCTCAGCAAAATCAAGTACTCAGGGGGGCCCCAGATTGTCAAGAAGGA
  GCGACGGCAAAGCTCCTCCCGCTTCAACCTCAGCAAGAATCGGGAGCTGCAGAAGCTTCC
  TGCCCTGAAAGATTCGCCAACCCAGGAGCGGGAGGAGCTGTTTATCCAGAAGCTACGCCA
  GTGCTGTGTCCTCTTTGACTTCGTGTCAGACCCACTCAGTGACCTCAAATTCAAGGAGGT
  GAAGCGGGCAGGACTCAACGAGATGGTGGAGTACATCACCCATAGCCGTGATGTTGTCAC
  TGAGGCCATTTACCCTGAGGCTGTCACCATGTTTTCAGTGAACCTCTTCCGGACGCTGNC
  ACCTTCATCGAATCCCACAGGGGCTGAGTTNGACCCAGAGGAAGATGAGCCCACCCTGGA
  AGCTGCTTTGCCACATCTCCAGCTCGTGTATGAGTTCTTCCTACGTTTCCTTGAGTCTCC
  TGATTCCAGCCAACATAGCCAGGAGTACATCGACCAGAAGTTGTACTTGCTCTCCTAGAC
  TATTTGAAGTNGAGATCCTCGAGAGCGGGACTTCTCAGAACATTTGCATTGCTNTTTNGC
  AAAGTTTTGGGGCTCCGGCTNATATCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006245 unedited
  CGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTCTCAGACACCCACTTT
  ATTCAGTTCTGTACATATGGGGACATCGGTCCAAGCCCAACCCACCTTAGCATGTATCAC
  TCTGTGGAGAATAAAGCACCCTATGTACACAGCCAAAAGCCGCACTGCCTGCGCCCCTGG
  AACCTGGGTCCGGCCTTCCTCAGCCCCTGGCCCCTCCAAGGTACCTATAAGGGACAAGAG
  AAGGCCTGCCCAGCATAGCACAAACAAGGAAATAAATAGGTGTTGGCAGGAGCCATGCTT
  GGGGCACCGCTCCCTGCTTTCCTTCTTTCTAGTTTTGGGAACCAACAGCACAGAAAAGCA
  GTCAGCCCCAGGGTGGTCCCTTCCATGTCTGTGACCCCAGGGCTGCTGCTCACCAAAGAC
  AGGGCACGGNGTAGGGTGTTTATTCATCCCTCCCCGGCCCCACTTGGTCCATCTGGGCCC
  TATATTCATCAGTTATTGTCCACAGCCTATACTTTTCTCCTTTTGACCTCCCTTGTCAGG
  CATGGGGTAAGAAGAAATAATACACCCCTATCTGCCAGCACACGGCTGGGACTGGGGGGG
  CCTATTTGTGCTGCAGGGGGTTGGGCTTCCNTTCACTACTCTGGCACCCATGGGGCTTGA
  CATAACTTTGGTGAGAATATCTCCTGCTGTTCCCTACATGCCTNTGCAGGNGAAAAAGAG
  AGAGGGAGATACCTGCCGTTCAATGAGGATGAAGGAANCAATANAGGACCGTATGTTTCC
  CTGGGTGTCCTTCTAAAGCACCCTCAGGACCTGGGGGTGAGGAGCAAGTAGGGCACAGGC
  TNCCCAGTTGCCGGACACTAGCATTTTATGCTCACCTGACTAGCCCAGGGAGCCTGACAC
  CCTTGGGGAAAGAGGCACATTTGGTGGGGTCCTGCTCAAGGCCCCAACCCCCCCTGGAAG
  CTAAAAGCCTGCCTCCCCAACAGCCTTAGNACAAATTGAAGGCCGGCAGGTCCAGCTGTG
  GCTTN
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_006245
• Insert Size: 3100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006245.2, NP_006236.1
• RefSeq Size: 2975 bp
• RefSeq ORF: 1809 bp
• Locus ID: 5528
• Cytogenetics: 6p21.1
• Domains: B56
• Protein Families: Phosphatase
• Protein Pathways: Oocyte meiosis, Wnt signaling pathway
• Gene Summary: 'The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a delta isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longest transcript, and encodes the longest isoform (1).