PPP2R5D (NM_006245) Human Untagged Clone

CAT#: SC324146

PPP2R5D (untagged)-Human protein phosphatase 2, regulatory subunit B', delta (PPP2R5D), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP2R5D
• Synonyms: B56D; B56delta; MRD35
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006245.2 GGCCGAGTGCGGCCGAGCAAAGCCGGAGCCGGAGCGGGGCCGCAGGAGACGGGCCGGGTC
  CGGACGGGCCGAGATGCCCTATAAACTGAAAAAGGAGAAGGAGCCCCCCAAGGTTGCCAA
  ATGCACAGCCAAGCCTAGCAGCTCGGGCAAGGATGGTGGAGGCGAGAACACTGAGGAGGC
  CCAGCCGCAGCCCCAGCCCCAGCCCCAGCCCCAAGCCCAGTCTCAGCCACCGTCATCCAA
  CAAGCGTCCCAGCAATAGCACGCCGCCCCCCACGCAGCTCAGCAAAATCAAGTACTCAGG
  GGGGCCCCAGATTGTCAAGAAGGAGCGACGGCAAAGCTCCTCCCGCTTCAACCTCAGCAA
  GAATCGGGAGCTGCAGAAGCTTCCTGCCCTGAAAGATTCGCCAACCCAGGAGCGGGAGGA
  GCTGTTTATCCAGAAGCTACGCCAGTGCTGTGTCCTCTTTGACTTCGTGTCAGACCCACT
  CAGTGACCTCAAATTCAAGGAGGTGAAGCGGGCAGGACTCAACGAGATGGTGGAGTACAT
  CACCCATAGCCGTGATGTTGTCACTGAGGCCATTTACCCTGAGGCTGTCACCATGTTTTC
  AGTGAACCTCTTCCGGACGCTGCCACCTTCATCGAATCCCACAGGGGCTGAGTTTGACCC
  AGAGGAAGATGAGCCCACCCTGGAAGCTGCTTGGCCACATCTCCAGCTCGTGTATGAGTT
  CTTCTTACGTTTCCTTGAGTCTCCTGATTTCCAGCCAAACATAGCCAAGAAGTACATCGA
  CCAGAAGTTTGTACTTGCTCTCCTAGACCTATTTGACAGTGAGGATCCTCGAGAGCGGGA
  CTTCCTCAAGACCATTTTGCATCGCATCTATGGCAAGTTTTTGGGGCTCCGGGCTTATAT
  CCGTAGGCAGATCAACCACATCTTCTACAGGTTCATCTACGAGACGGAGCATCACAACGG
  GATTGCTGAGCTCCTGGAGATCCTGGGCAGCATCATCAATGGCTTTGCCCTGCCCCTTAA
  AGAAGAGCACAAGATGTTCCTCATCCGTGTCCTACTTCCCCTTCACAAGGTCAAGTCCCT
  GAGTGTCTACCACCCTCAGCTGGCATACTGTGTGGTACAATTCCTGGAGAAGGAGAGCAG
  TCTGACTGAGCCGGTAATTGTGGGACTTCTCAAGTTTTGGCCCAAGACCCACAGCCCCAA
  GGAGGTGATGTTCTTGAATGAGCTGGAGGAGATTCTGGACGTCATTGAACCTTCTGAGTT
  CAGCAAAGTGATGGAACCCCTCTTCCGCCAGCTGGCCAAGTGTGTCTCTAGCCCCCATTT
  CCAGGTGGCAGAGCGTGCTCTCTATTACTGGAACAATGAGTACATCATGAGCCTGATAAG
  TGACAATGCTGCCCGAGTCCTCCCCATCATGTTCCCTGCACTCTACAGGAACTCCAAGAG
  CCACTGGAACAAGACAATCCATGGACTGATCTATAATGCCCTGAAGTTGTTTATGGAAAT
  GAATCAGAAGCTGTTTGATGACTGCACACAACAATACAAGGCAGAGAAGCAGAAGGGCCG
  GTTCCGAATGAAGGAAAGGGAAGAGATGTGGCAAAAAATCGAGGAGCTGGCCCGGCTTAA
  TCCCCAGTATCCCATGTTCCGAGCCCCTCCACCACTGCCCCCTGTGTACTCGATGGAGAC
  AGAGACCCCCACAGCTGAGGACATCCAGCTTCTGAAGAGGACTGTGGAGACTGAGGCTGT
  TCAGATGCTAAAAGACATCAAGAAGGAGAAAGTGCTGCTGCGGAGGAAGTCGGAGCTGCC
  CCAGGACGTGTACACCATCAAGGCACTGGAGGCGCACAAGCGGGCGGAAGAGTTCCTAAC
  TGCCAGCCAGGAGGCTCTCTGACCCCTCACGTTCCTACCACAGGGCCACAGCCCACACAG
  CCCTGGGACACTGCCCTGGCCCTCCATACTCTGCTCCCTACTGGCTGTCTTGGGGGAAGG
  CAGCGCCTCTCTAGCTACTCAAGGGAGGGGGATGTGGGCACTTGAAGCAGGGACACCCAC
  AGAATGGTCCCTCTTCTCCCCAAAAGGTGTTCATGCCTCCCTGTGGCTAGTACAGGCTGA
  GCACTAAGATGCTTAGTGCTCAGACAACCTGGGGATGCCTGTCCCCTACCTGCTCCTCAC
  CCACAGCTACCTGAGGCTGCTCTGAGAAGTACACACAGGAATACATACGCTCCTCTATTC
  TTCCCTTCATCCTCATTTGAACGCCAGGTATCTCCCCTCCTCTCTCTCCCCTGCAGAGGC
  ATGTAGGGAACAGCAGGAGATTATTCTCACCAAAGTTATGTCAAGCCCCATTGGTCCCAG
  AGTAGCTGAAGGGAAGCCAACCCCCCTGCAGCACAAATAGGCCCCCCAGTCCCAGCCGTG
  TGCTGGCAGATAGGGTTGTATTATTTCTTCTTACCCCATGCCTGACCAAGGGAGGTCAAA
  AGGAGAAAAGTATAGGCTGTGGACAATAACTGATGAATATAGGGCCCAGATGGACCAAGT
  GGGGCCGGGGAGGGATGAATAAACACCCTACCCCGTGCCCTGTCTTTGGTGAGCAGCAGC
  CCTGGGGTCACAGACATGGAAGGGACCACCCTGGGGCTGACTGCTTTTCTGTGCTGTTGG
  TTCCCAAAACTAGAAAGAAGGAAGCAGGGAGCGGTGCCCCAAGCATGGCTCCTGCCAACA
  CCTATTTATTTCCTTGTTTGTGCTATGCTGGGCAGGCCTTCTCTTGTCCCTTATAGGTAC
  CTTGGAGGGGCCAGGGGCTGAGGAAGGCCGGACCCAGGTTCCAGGGGCGCAGGCAGTGCG
  GCTTTTGGCTGTGTACATAGGGTGCTTTATTCTCCACAGAGTGATACATGCTAAGGTGGG
  TTGGGCTTGGACCGATGTCCCCATATGTACAGAACTGAATAAAGTGGGTCTCTGAGAAAA
  AAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_006245
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006245.2, NP_006236.1
• RefSeq Size: 2975 bp
• RefSeq ORF: 1809 bp
• Locus ID: 5528
• Cytogenetics: 6p21.1
• Domains: B56
• Protein Families: Phosphatase
• Protein Pathways: Oocyte meiosis, Wnt signaling pathway
• Gene Summary: 'The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a delta isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longest transcript, and encodes the longest isoform (1).