Granzyme A (GZMA) (NM_006144) Human Untagged Clone

CAT#: SC116272

GZMA (untagged)-Human granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3) (GZMA)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GZMA
• Synonyms: CTLA3; HFSP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116272 sequence for NM_006144 edited (data generated by NextGen Sequencing)
  ATGAGGAACTCCTATAGATTTCTGGCATCCTCTCTCTCAGTTGTCGTTTCTCTCCTGCTA
  ATTCCTGAAGATGTCTGTGAAAAAATTATTGGAGGAAATGAAGTAACTCCTCATTCAAGA
  CCCTACATGGTCCTACTTAGTCTTGACAGAAAAACCATCTGTGCTGGGGCTTTGATTGCA
  AAAGACTGGGTGTTGACTGCAGCTCACTGTAACTTGAACAAAAGGTCCCAGGTCATTCTT
  GGGGCTCACTCAATAACCAGGGAAGAGCCAACAAAACAGATAATGCTTGTTAAGAAAGAG
  TTTCCCTATCCATGCTATGACCCAGCCACACGCGAAGGTGACCTTAAACTTTTACAGCTG
  ACGGAAAAAGCAAAAATTAACAAATATGTGACTATCCTTCATCTACCTAAAAAGGGGGAT
  GATGTGAAACCAGGAACCATGTGCCAAGTTGCAGGGTGGGGCAGGACTCACAATAGTGCA
  TCTTGGTCCGATACTCTGAGAGAAGTCAATATCACCATCATAGACAGAAAAGTCTGCAAT
  GATCGAAATCACTATAATTTTAACCCTGTGATTGGAATGAATATGGTTTGTGCTGGAAGC
  CTCCGAGGTGGAAGAGACTCGTGCAATGGAGATTCTGGAAGCCCTTTGTTGTGCGAGGGT
  GTTTTCCGAGGGGTCACTTCCTTTGGCCTTGAAAATAAATGCGGAGACCCTCGTGGGCCT
  GGTGTCTATATTCTTCTCTCAAAGAAACACCTCAACTGGATAATTATGACTATCAAGGGA
  GCAGTTTAA
  
  Clone variation with respect to NM_006144.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006144 unedited
  CCGCGAATTCGGCACGAGCCACAATGAGGAACTCCTATAGATTTCTGGCATCCTCTCTCT
  CAGTTGTCGTTTCTCTCCTGCTAATTCCTGAAGATGTCTGTGAAAAAATTATTGGAGGAA
  ATGAAGTAACTCCTCATTCAAGACCCTACATGGTCCTACTTAGTCTTGACAGAAAAACCA
  TCTGTGCTGGGGCTTTGATTGCAAAAGACTGGGTGTTGACTGCAGCTCACTGTAACTTGA
  ACAAAAGGTCCCAGGTCATTCTTGGGGCTCACTCAATAACCAGGGAAGAGCCAACAAAAC
  AGATAATGCTTGTTAAGAAAGAGTTTCCCTATCCATGCTATGACCCAGCCACACGCGAAG
  GTGACCTTAAACTTTTACAGCTGACGGAAAAAGCAAAAATTAACAAATATGTGACTATCC
  TTCATCTACCTAAAAAGGGGGATGATGTGAAACCAGGAACCATGTGCCAAGTTGCAGGGT
  GGGGCAGGACTCACAATAGTGCATCTTGGTCCGATACTCTGAGAGAAGTCAATATCACCA
  TCATAGACAGAAAAGTCTGCAATGATCGAAATACTATAATTTTAACCCTGTGATTGGATG
  AATATTGGTTTGTGCTGGAAGCCTNCGAGGTGGAAGAGACTCGTGCATGGAGATTCTGGA
  AGCCTTTTGTTGTGCAAGGGTGTTNTCGGAGGGTCACTTTCTTTGCCTTGAAATAATGCG
  GAACCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006144 unedited
  NTGTCTAGCAAATGGTTTTATTTTGTGAAAGNTATAAGNAAGCCACCAGTAAATGGAAGG
  GAAACGGTTATTTAAACTGCTCCCTTGATAGTCATAATTATCCAGTTGAGGTGTTTCTTT
  GAGAGAAGAATATAGACACCAGGCCCACGAGGGTCTCCGCATTTATTTTCAAGGCCAAAG
  GAAGTGACCCCTCGGAAAACACCCTCGCACAACAAAGGGCTTCCAGAATCTCCATTGCAC
  GAGTCTCTTCCACCTCGGAGGCTTCCAGCACAAACCATATTCATTCCAATCACAGGGTTA
  AAATTATAGTGATTTCGATCATTGCAGACTTTTCTGTCTATGATGGTGATATTGACTTCT
  CTCAGAGTATCGGACCAAGATGCACTATTGTGAGTCCTGCCCCACCCTGCAACTTGGCAC
  ATGGTTCCTGGTTTCACATCATCCCCCTTTTTAGGTAGATGAAGGATAGTCACATATTTG
  TTAATTTTTGCTTTTTCCGTCAGCTGTAAAAGTTTAAGGTCACCTTCGCGTGTGGCTGGG
  TCATAGCATGGATAGGGAAACTCTTTCTTAACAAGCATTATCTGTTTTGTTGGCTCTTCC
  CTGGTTATTGAGTGAGCCCCAAGAATGACCTGNGACCTTTTGTTCAAGTTACAGTGAGCT
  GCAGTCAACACCCAGTCTTTTGCAATCAAAGCCCCAGCACAGATGGTTTTTTCTGTCAAG
  ACTAAGTAAGGACATTGTAGGTCTTGAATGAAGGAGTACTTCATTTTCTCCCATAATTTT
  TTCACAGACCTCTTTCGGGATAACCAGGAGAGAAACGACACTGAGAGAGGAGATGCCAGA
  AATCTATTAGAGTTCTTCATTGTGGGTCGTGCCGAAATTCCCGCCGCCCTTAAAGGAGCG
  TTTTACAAATTTTGCGGGTTACTAAACAGCTCTGCTTTATAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006144
• Insert Size: 950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006144.2, NP_006135.1
• RefSeq Size: 878 bp
• RefSeq ORF: 789 bp
• Locus ID: 3001
• Cytogenetics: 5q11.2
• Domains: Tryp_SPc
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: 'Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells. [provided by RefSeq, Jul 2008]'