CSK (NM_004383) Human Untagged Clone

CAT#: SC117389

CSK (untagged)-Human c-src tyrosine kinase (CSK), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CSK
• Synonyms: MGC117393
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004383 edited
  ATGTCAGCAATACAGGCCGCCTGGCCATCCGGTACAGAATGTATTGCCAAGTACAACTTC
  CACGGCACTGCCGAGCAGGACCTGCCCTTCTGCAAAGGAGACGTGCTCACCATTGTGGCC
  GTCACCAAGGACCCCAACTGGTACAAAGCCAAAAACAAGGTGGGCCGTGAGGGCATCATC
  CCAGCCAACTACGTCCAGAAGCGGGAGGGTGTGAAGGCGGGTACCAAACTCAGCCTCATG
  CCTTGGTTCCACGGCAAGATCACACGGGAGCAGGCTGAGCGGCTTCTGTACCCGCCGGAG
  ACAGGCCTGTTCCTGGTGCGGGAGAGCACCAACTACCCCGGAGACTACACGCTGTGCGTG
  AGCTGCGACGGCAAGGTGGAGCACTACCGCATCATGTACCATGCCAGCAAGCTCAGCATC
  GACGAGGAGGTGTACTTTGAGAACCTCATGCAGCTGGTGGAGCACTACACCTCAGACGCA
  GATGGACTCTGTACGCGCCTCATTAAACCAAAGGTCATGGAGGGCACAGTGGCGGCCCAG
  GATGAGTTCTACCGCAGCGGCTGGGCCCTGAACATGAAGGAGCTGAAGCTGCTGCAGACC
  ATCGGGAAGGGGGAGTTCGGAGACGTGATGCTGGGCGATTACCGAGGGAACAAAGTCGCC
  GTCAAGTGCATTAAGAACGACGCCACTGCCCAGGCCTTCCTGGCTGAAGCCTCAGTCATG
  ACGCAACTGCGGCATAGCAACCTGGTGCAGCTCCTGGGCGTGATCGTGGAGGAGAAGGGC
  GGGCTCTACATCGTCACTGAGTACATGGCCAAGGGGAGCCTTGTGGACTACCTGCGGTCT
  AGGGGTCGGTCAGTGCTGGGCGGAGACTGTCTCCTCAAGTTCTCGCTAGATGTCTGCGAG
  GCCATGGAATACCTGGAGGGCAACAATTTCGTGCATCGAGACCTGGCTGCCCGCAATGTG
  CTGGTGTCTGAGGACAACGTGGCCAAGGTCAGCGACTTTGGTCTCACCAAGGAGGCGTCC
  AGCACCCAGGACACGGGCAAGCTGCCAGTCAAGTGGACAGCCCCTGAGGCCCTGAGAGAG
  AAGAAATTCTCCACTAAGTCTGACGTGTGGAGTTTCGGAATCCTTCTCTGGGAAATCTAC
  TCCTTTGGGCGAGTGCCTTATCCAAGAATTCCCCTGAAGGACGTCGTCCCTCGGGTGGAG
  AAGGGCTACAAGATGGATGCCCCCGACGGCTGCCCGCCCGCAGTCTATGAAGTCATGAAG
  AACTGCTGGCACCTGGACGCCGCCATGCGGCCCTCCTTCCTACAGCTCCGAGAGCAGCTT
  GAGCACATCAAAACCCACGAGCTGCACCTGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004383 unedited
  CCGCACGAGGCCCTCGTGCCGAATTCGGCACGAGGGCGGCCCCGCACGAGCGTCCGGGGC
  GGCCCCCGGCAGCCAGCGCGACCTTCCAAAATCGAACCTCAGTGGCGGCGCTCGAGAACC
  GGAACTCTGCCGGGGCCGCGCCGGCTACATTGTTTCCTCCCCCCGACTCCCTCCCGCCCC
  CTTCCCCCGCCTTTCTTCCCTCCGCGACCCGGGCCGTGCGTCCGTCCCCCTGCCTCTGCC
  TGGCGGTCCCTCCTCCCCTCTCCTTGCACCCATACCTCTTTGTACCGCACCCCCTGGGGA
  CCCCTGCGCCCCTCCTCTCCCCCCTGACCGCATGGACCGTCCCGCAAGCCGCTGATGCCG
  CCCGCAGCTAGGTGGCCCAGACCGCAATGCCCCAAGAGAGCTCTAATGGTACCAAGTGAC
  AAGTTGGCTTTACTGTGACTCGGGGACTCCAGAGCTCCTGATAAGATGTCAGCAATACAA
  GCCGCCTGGCCATCCGGTACATAATGTATTGCCAAGTACAACTTCCACGGCACTGCCGAG
  CATGACCTGCCCTTCTGCAAAGGATACGTGCTCACCATTGTGGCCGTCACCAAGGACCCC
  AACTGGTACAAAGCCGATAACAAGGTGGCGCAGTGAGGGCATCATCCCAGCCAACTACAT
  CCATAAGCAGGATGGTGTGAAGGCAGGTACCAAACTCAGCCTCATGCCTTGGTTCCACGA
  GCAGATCACACAGGAGCTAGCTGATGCGCTTCTGTACCCGCCGATACAGGACTGATCCTG
  GTGCAGGAGAGCACATCTACCCTGCATCTACACGCTGTGCATGATCTGCGACTGCNAAGT
  GGAGCACTACCTCATCATGTGCATGACAGCACTCTCATCGTCGACGAGGACGTGTACTTT
  GAGAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004383
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004383.1, NP_004374.1
• RefSeq Size: 2420 bp
• RefSeq ORF: 1353 bp
• Locus ID: 1445
• Cytogenetics: 15q24.1
• Domains: pkinase, SH2, TyrKc, SH3, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, Neurotrophin signaling pathway, Regulation of actin cytoskeleton
• Gene Summary: 'The protein encoded by this gene is involved in multiple pathways, including the regulation of Src family kinases. It plays an important role in T-cell activation through its association with the protein encoded by the protein tyrosine phosphatase, non-receptor type 22 (PTPN22) gene. This protein also phosphorylates C-terminal tyrosine residues on multiple substrates, including the protein encoded by the SRC proto-oncogene, non-receptor tyrosine kinase gene. Phosphorylation suppresses the kinase activity of the Src family tyrosine kinases. An intronic polymorphism (rs34933034) in this gene has been found to affect B-cell activation and is associated with systemic lupus erythematosus (SLE). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2017]'
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same protein.