CSK (NM_004383) Human Untagged Clone

CAT#: SC323685

CSK (untagged)-Kinase deficient mutant (K222M) of Human c-src tyrosine kinase (CSK), transcript variant 1


  "NM_004383" in other vectors (7)

Reconstitution Protocol

USD 760.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CSK
Synonyms MGC117393
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC323685 sequence for NM_004383 edited (data generated by NextGen Sequencing)
ATGTCAGCAATACAGGCCGCCTGGCCATCCGGTACAGAATGTATTGCCAAGTACAACTTC
CACGGCACTGCCGAGCAGGACCTGCCCTTCTGCAAAGGAGACGTGCTCACCATTGTGGCC
GTCACCAAGGACCCCAACTGGTACAAAGCCAAAAACAAGGTGGGCCGTGAGGGCATCATC
CCAGCCAACTACGTCCAGAAGCGGGAGGGTGTGAAGGCGGGTACCAAACTCAGCCTCATG
CCTTGGTTCCACGGCAAGATCACACGGGAGCAGGCTGAGCGGCTTCTGTACCCGCCGGAG
ACAGGCCTGTTCCTGGTGCGGGAGAGCACCAACTACCCCGGAGACTACACGCTGTGCGTG
AGCTGCGACGGCAAGGTGGAGCACTACCGCATCATGTACCATGCCAGCAAGCTCAGCATC
GACGAGGAGGTGTACTTTGAGAACCTCATGCAGCTGGTGGAGCACTACACCTCAGACGCA
GATGGACTCTGTACGCGCCTCATTAAACCAAAGGTCATGGAGGGCACAGTGGCGGCCCAG
GATGAGTTCTACCGCAGCGGCTGGGCCCTGAACATGAAGGAGCTGAAGCTGCTGCAGACC
ATCGGGAAGGGGGAGTTCGGAGACGTGATGCTGGGCGATTACCGAGGGAACAAAGTCGCC
GTCATGTGCATTAAGAACGACGCCACTGCCCAGGCCTTCCTGGCTGAAGCCTCAGTCATG
ACGCAACTGCGGCATAGCAACCTGGTGCAGCTCCTGGGCGTGATCGTGGAGGAGAAGGGC
GGGCTCTACATCGTCACTGAGTACATGGCCAAGGGGAGCCTTGTGGACTACCTGCGGTCT
AGGGGTCGGTCAGTGCTGGGCGGAGACTGTCTCCTCAAGTTCTCGCTAGATGTCTGCGAG
GCCATGGAATACCTGGAGGGCAACAATTTCGTGCATCGAGACCTGGCTGCCCGCAATGTG
CTGGTGTCTGAGGACAACGTGGCCAAGGTCAGCGACTTTGGTCTCACCAAGGAGGCGTCC
AGCACCCAGGACACGGGCAAGCTGCCAGTCAAGTGGACAGCCCCTGAGGCCCTGAGAGAG
AAGAAATTCTCCACTAAGTCTGACGTGTGGAGTTTCGGAATCCTTCTCTGGGAAATCTAC
TCCTTTGGGCGAGTGCCTTATCCAAGAATTCCCCTGAAGGACGTCGTCCCTCGGGTGGAG
AAGGGCTACAAGATGGATGCCCCCGACGGCTGCCCGCCCGCAGTCTATGAAGTCATGAAG
AACTGCTGGCACCTGGACGCCGCCATGCGGCCCTCCTTCCTACAGCTCCGAGAGCAGCTT
GAGCACATCAAAACCCACGAGCTGCACCTGTGA

Clone variation with respect to NM_004383.2
210 c=>t;665 a=>t
>OriGene 5' read for mutant NM_004383 unedited
ACGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATT
CGGCACGAGGGCGGCCCCGATCGAGCGTCCGGGGCGGCCCCCGGCAGCCAGCGCGACGTTCCAAAATCGA
ACCTCAGTGGCGGCGCTCGGAAGCGGAACTCTGCCGGGGCCGCGCCGGCTACATTGTTTCCTCCCCCCGA
CTCCCTCCCGCCCCCTTCCCCCGCCTTTCTTCCCTCCGCGACCCGGGCCGTGCGTCCGTCCCCCCTGCCT
CTGCCTGGCGGGTCCCCTCCTCCCCCTCCTCCTTGCCACCCCATAACCTCTTTTGTTACCCGCACCCCCT
GTGGGACCCCCTGGGCGCCCCTTCCCCTCCCCCCTTGGACCGCATTGGAACCCGTCCCGGCAGGCCGGTT
GAAGGCCCCCCCGGGGGCAAAGTTGGCCCCGGACCCCGCAGTTGCCCCAGAAAAGGCTTTTATTGTTCCC
AGTTTGAAAGGGTGGGGCTTTTACGGGACATTGGGGGGGACGCCAGAAAACCCTCCGGAAAAGAATGTGC
CCAACACGGGCCCGCCGGGGCCATCCGTGCCCAAAAGTGTTTGCCAATTTAAATTTTACGGGACTGTCGA
AAAACGAGACGTGCCTTTGCGAAGGAGAAATGTCTCACTTTTGGGGCCCGCCACGAGCCCCCCCTGGTGC
AAAAGCACAACACGTGGGGGCCCGGAGGGGGGTCTTCCCCCCCCACTACTTCCTCAAACCCCGAGGTGTG
TAAGACGCTATCACACATCATCCTCATTGCCGTGGTGTTCTCCGGCGGATATCTACCGGGACAGCGTGAT
CAGCCTTTTTTACACCCGCAGAAAGCCTGTTCTCTGTGTGGTGGAAAACCACATACTACGAGAGCATACT
AACCTGCGTCTTGAGCTGCTCACGCAAGGTGTACACATTCCAGCACTATGTAGATGCGCGCCAGG
>SC323685 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CGCTGACTGACTGAAGGAGCTGAGCTGCTGCAGACCATCGGGAAGGGGGAGTTCGGAGACKTGATGCTGG
GCGATTACCGAGGGAACAAAGTCGCCGTCATGTGCATTAAGAACGACGCCACTGCCCAGGCCTTCCTGGC
TGAAGCCTCAGTCATGACGCAACTGCGGCATAGCAACCTGGTGCAGCTCCTGGGCGTGATCGTGGAGGAG
AAGGGCGGGCTCTACATCGTCACTGAGTACATGGCCAAGGGGAGC
Restriction Sites Please inquire     
ACCN NM_004383
Insert Size 2500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004383.1, NP_004374.1
RefSeq Size 2420 bp
RefSeq ORF 1353 bp
Locus ID 1445
Cytogenetics 15q24.1
Domains pkinase, SH2, TyrKc, SH3, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Chemokine signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, Neurotrophin signaling pathway, Regulation of actin cytoskeleton
Gene Summary 'The protein encoded by this gene is involved in multiple pathways, including the regulation of Src family kinases. It plays an important role in T-cell activation through its association with the protein encoded by the protein tyrosine phosphatase, non-receptor type 22 (PTPN22) gene. This protein also phosphorylates C-terminal tyrosine residues on multiple substrates, including the protein encoded by the SRC proto-oncogene, non-receptor tyrosine kinase gene. Phosphorylation suppresses the kinase activity of the Src family tyrosine kinases. An intronic polymorphism (rs34933034) in this gene has been found to affect B-cell activation and is associated with systemic lupus erythematosus (SLE). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2017]'
Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same protein.

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