BAG3 (NM_004281) Human Untagged Clone

CAT#: SC117478

BAG3 (untagged)-Human BCL2-associated athanogene 3 (BAG3)


  "NM_004281" in other vectors (7)

Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol BAG3
Synonyms BAG-3; BIS; CAIR-1; MFM6
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_004281, the custom clone sequence may differ by one or more nucleotides


ATGAGCGCCGCCACCCACTCGCCCATGATGCAGGTGGCGTCCGGCAACGGTGACCGCGACCCTTTGCCCC
CCGGATGGGAGATCAAGATCGACCCGCAGACCGGCTGGCCCTTCTTCGTGGACCACAACAGCCGCACCAC
TACGTGGAACGACCCGCGCGTGCCCTCTGAGGGCCCCAAGGAGACTCCATCCTCTGCCAATGGCCCTTCC
CGGGAGGGCTCTAGGCTGCCGCCTGCTAGGGAAGGCCACCCTGTGTACCCCCAGCTCCGACCAGGCTACA
TTCCCATTCCTGTGCTCCATGAAGGCGCTGAGAACCGGCAGGTGCACCCTTTCCATGTCTATCCCCAGCC
TGGGATGCAGCGATTCCGAACTGAGGCGGCAGCAGCGGCTCCTCAGAGGTCCCAGTCACCTCTGCGGGGC
ATGCCAGAAACCACTCAGCCAGATAAACAGTGTGGACAGGTGGCAGCGGCGGCGGCAGCCCAGCCCCCAG
CCTCCCACGGACCTGAGCGGTCCCAGTCTCCAGCTGCCTCTGACTGCTCATCCTCATCCTCCTCGGCCAG
CCTGCCTTCCTCCGGCAGGAGCAGCCTGGGCAGTCACCAGCTCCCGCGGGGGTACATCTCCATTCCGGTG
ATACACGAGCAGAACGTTACCCGGCCAGCAGCCCAGCCCTCCTTCCACCAAGCCCAGAAGACGCACTACC
CAGCGCAGCAGGGGGAGTACCAGACCCACCAGCCTGTGTACCACAAGATCCAGGGGGATGACTGGGAGCC
CCGGCCCCTGCGGGCGGCATCCCCGTTCAGGTCATCTGTCCAGGGTGCATCGAGCCGGGAGGGCTCACCA
GCCAGGAGCAGCACGCCACTCCACTCCCCCTCGCCCATCCGTGTGCACACCGTGGTCGACAGGCCTCAGC
AGCCCATGACCCATCGAGAAACTGCACCTGTTTCCCAGCCTGAAAACAAACCAGAAAGTAAGCCAGGCCC
AGTTGGACCAGAACTCCCTCCTGGACACATCCCAATTCAAGTGATCCGCAAAGAGGTGGATTCTAAACCT
GTTTCCCAGAAGCCCCCACCTCCCTCTGAGAAGGTAGAGGTGAAAGTTCCCCCTGCTCCAGTTCCTTGTC
CTCCTCCCAGCCCTGGCCCTTCTGCTGTCCCCTCTTCCCCCAAGAGTGTGGCTACAGAAGAGAGGGCAGC
CCCCAGCACTGCCCCTGCAGAAGCTACACCTCCAAAACCAGGAGAAGCCGAGGCTCCCCCAAAACATCCA
GGAGTGCTGAAAGTGGAAGCCATCCTGGAGAAGGTACAGGGGCTGGAGCAGGCTGTAGACAACTTTGAAG
GCAAGAAGACTGACAAAAAGTACCTGATGATCGAAGAGTATTTGACCAAAGAGCTGCTGGCCCTGGATTC
AGTGGACCCCGAGGGACGAGCCGATGTGCGTCAGGCCAGGAGAGACGGTGTCAGGAAGGTTCAGACCATC
TTGGAAAAACTTGAACAGAAAGCCATTGATGTCCCAGGTCAAGTCCAGGTCTATGAACTCCAGCCCAGCA
ACCTTGAAGCAGATCAGCCACTGCAGGCAATCATGGAGATGGGTGCCGTGGCAGCAGACAAGGGCAAGAA
AAATGCTGGAAATGCAGAAGATCCCCACACAGAAACCCAGCAGCCAGAAGCCACAGCAGCAGCGACTTCA
AACCCCAGCAGCATGACAGACACCCCTGGTAACCCAGCAGCACCGTAG


>OriGene 5' read for NM_004281 unedited
GTAAACGTCAAAATTTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCGA
GGAGGCTATTTCCAGNAACTTCCACCCCTCTCTGGCCACGTCACCCCCGCCTTTAATTCA
TAAAGGTGCCCGGCGCCGGCTTCCCGGACACGTCGGCGGCGGAGAGGGGCCCACGGCGGC
GGCCCCGGCCAGAGACTCGGCGCCCGGAGCCAGCGCCCCGCACCCGCGCCCCAGCGGGCA
GACCCCAACCCAGCATGAGCGCCGCCACCCACTCGCCCATGATGCAGGTGGCGTCCGGCA
AACGGTGACCGCGACCCTTTGCCCCCCGGATGGGAGATCAAGATCGACCCCGCAGACCGG
GCTGGCCTTTCTTCGTGGGACCACAACAGCCGCACCACTACGTGGAACGACCCGCGCGTG
CCCTCTGAGGGCCCCAAAGAGACTCCATCCTCTGCCAATGGCCCTTCCCGGGAGGGCTCT
AGGCTGCCGCCTGCTAGGGAAAGCCACCCTGTGTACCCCCAGCTCCGACCAGGCTACATT
CCCATTTCCTGTGCNTCCATGAAGCGCTGANGACCGGCAGGTGCACCCTTTCATGTCTAT
CCCCACCTGGGATGCAGCGATTCCGAACTGAGGCGGCAGCAGCGGCTCCTCAGAGGTCCC
AGTCACCTCTGCGGGGCATGCCAGAAACCACTCAGCCAGATAAACAGTGTGGACAGGTGG
CAGCGGCGGCGGCAGCCCAGCCCCCAGCCTCCCACGGACCTGAGCGGTCCCAGTCTCCAG
CTGCCTCTGACTGCTCATCCTCATCCTCCTCGGCCAGCCTGCCTTCCTCCGGCAGGAGCA
GCCTGGGCAGTCACCAGCTCCCGCGGGGGTACATCTCCATTCCGGTGATACACGAGCAGA
ACGTTACCCCGCCAGC
>OriGene 3' read for NM_004281 unedited
ACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGTTATATTATTACTTTATTTTC
TTTTTTTAAAATGTAGCATTAAAGTCATCCAACATACAGATATTCCTATGGCTCCTGGCA
CATTTTACTCTCTCTAAAGTCAGGTATTTTAATTATGAGATGAAGAAAATCATCTCATTA
AAATGGCAACATTTCTGATAAATGTTTCATATTTATGTGATGGGTAATTGACTCCCCATC
TACCCCTCCAGTCCAGAGCTACAAAAGACAGTGCACAACCACAGCTAACAGGTGGTGGGG
GTGCCCAAGTAGACAGGGCTGCAGAACAACAAGCAACGGGGTTAAACTTCTCAAACAACA
AGCAACTTCTTTATTTGTACAGAGTAAGAATATAGAAGAAAAGCATCATTTTCCTTTTTA
GCCCTTTTATTAGTGTTTTGCCTCCACCCAAGTTACTGCATACCAAGCAGCTAATAAAAA
CCAACTGACTTAAAGTCTCTGAAATGCATGCAACTTATAATTCCCTAAAGCACACATCGG
TTCCGAGTCTGATTTTTACAGGGCATAAGCTACCGCGCTGCTGGGCTACCAGGGGTGTCT
GTCATGCCGCTGGGGTTTGAACCCCTGCTGCTGAGGCTTCTGGCCGCTGGGCTTCCGCGC
GGGGGATCTCTGCATCTCCAGCCCCTTTTTTGCCCTTTGCGGTTCCACGGGACCCCTTTC
CCTGATTGGCTTCACCCGCCACTCCCGCTCCCAGGGGCTCGGCTAGGATTCTACACCCCG
ACCTGCCCTGGACTTATTGCCTTCCTCGTAACTTTTTCATAAGGGCGGACCCTCTCCGAC
CAGGCTTTCTGTCTTCCACCATCACTTTCTCTCCCGGTCCACGATCCACGGACCTCTTCT
GTGACGATCTCAGCCGCGTAGACTCTTTGTACTACTCTCTTTAAATCTACCATTCCCTAC
TCCCCTCCCCCCCCGCTGCCCCTGG
Restriction Sites NotI-NotI     
ACCN NM_004281
ORF Size 1728 bp
Insert Size 2560
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Reference Data
RefSeq NM_004281.3, NP_004272.2
RefSeq Size 2608
RefSeq ORF 1728
Locus ID 9531
Domains WW, BAG
Protein Families Druggable Genome
Gene Summary BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008]

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