BAG3 (NM_004281) Human Untagged Clone

CAT#: SC320765

BAG3 (untagged)-Human BCL2-associated athanogene 3 (BAG3)


  "NM_004281" in other vectors (7)

Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol BAG3
Synonyms BAG-3; BIS; CAIR-1; MFM6
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_004281.3 GGGCCGCGGCCAACTTCTCTGGACTGGACCAGAAGTTTCTAGCCGGCCAGTTGCTACCTC
CCTTTATCTCCTCCTTCCCCTCTGGCAGCGAGGAGGCTATTTCCAGACACTTCCACCCCT
CTCTGGCCACGTCACCCCCGCCTTTAATTCATAAAGGTGCCCGGCGCCGGCTTCCCGGAC
ACGTCGGCGGCGGAGAGGGGCCCACGGCGGCGGCCCGGCCAGAGACTCGGCGCCCGGAGC
CAGCGCCCCGCACCCGCGCCCCAGCGGGCAGACCCCAACCCAGCATGAGCGCCGCCACCC
ACTCGCCCATGATGCAGGTGGCGTCCGGCAACGGTGACCGCGACCCTTTGCCCCCCGGAT
GGGAGATCAAGATCGACCCGCAGACCGGCTGGCCCTTCTTCGTGGACCACAACAGCCGCA
CCACTACGTGGAACGACCCGCGCGTGCCCTCTGAGGGCCCCAAGGAGACTCCATCCTCTG
CCAATGGCCCTTCCCGGGAGGGCTCTAGGCTGCCGCCTGCTAGGGAAGGCCACCCTGTGT
ACCCCCAGCTCCGACCAGGCTACATTCCCATTCCTGTGCTCCATGAAGGCGCTGAGAACC
GGCAGGTGCACCCTTTCCATGTCTATCCCCAGCCTGGGATGCAGCGATTCCGAACTGAGG
CGGCAGCAGCGGCTCCTCAGAGGTCCCAGTCACCTCTGCGGGGCATGCCAGAAACCACTC
AGCCAGATAAACAGTGTGGACAGGTGGCAGCGGCGGCGGCAGCCCAGCCCCCAGCCTCCC
ACGGACCTGAGCGGTCCCAGTCTCCAGCTGCCTCTGACTGCTCATCCTCATCCTCCTCGG
CCAGCCTGCCTTCCTCCGGCAGGAGCAGCCTGGGCAGTCACCAGCTCCCGCGGGGGTACA
TCTCCATTCCGGTGATACACGAGCAGAACGTTACCCGGCCAGCAGCCCAGCCCTCCTTCC
ACCAAGCCCAGAAGACGCACTACCCAGCGCAGCAGGGGGAGTACCAGACCCACCAGCCTG
TGTACCACAAGATCCAGGGGGATGACTGGGAGCCCCGGCCCCTGCGGGCGGCATCCCCGT
TCAGGTCATCTGTCCAGGGTGCATCGAGCCGGGAGGGCTCACCAGCCAGGAGCAGCACGC
CACTCCACTCCCCCTCGCCCATCCGTGTGCACACCGTGGTCGACAGGCCTCAGCAGCCCA
TGACCCATCGAGAAACTGCACCTGTTTCCCAGCCTGAAAACAAACCAGAAAGTAAGCCAG
GCCCAGTTGGACCAGAACTCCCTCCTGGACACATCCCAATTCAAGTGATCCGCAAAGAGG
TGGATTCTAAACCTGTTTCCCAGAAGCCCCCACCTCCCTCTGAGAAGGTAGAGGTGAAAG
TTCCCCCTGCTCCAGTTCCTTGTCCTCCTCCCAGCCCTGGCCCTTCTGCTGTCCCCTCTT
CCCCCAAGAGTGTGGCTACAGAAGAGAGGGCAGCCCCCAGCACTGCCCCTGCAGAAGCTA
CACCTCCAAAACCAGGAGAAGCCGAGGCTCCCCCAAAACATCCAGGAGTGCTGAAAGTGG
AAGCCATCCTGGAGAAGGTACAGGGGCTGGAGCAGGCTGTAGACAACTTTGAAGGCAAGA
AGACTGACAAAAAGTACCTGATGATCGAAGAGTATTTGACCAAAGAGCTGCTGGCCCTGG
ATTCAGTGGACCCCGAGGGACGAGCCGATGTGCGTCAGGCCAGGAGAGACGGTGTCAGGA
AGGTTCAGACCATCTTGGAAAAACTTGAACAGAAAGCCATTGATGTCCCAGGTCAAGTCC
AGGTCTATGAACTCCAGCCCAGCAACCTTGAAGCAGATCAGCCACTGCAGGCAATCATGG
AGATGGGTGCCGTGGCAGCAGACAAGGGCAAGAAAAATGCTGGAAATGCAGAAGATCCCC
ACACAGAAACCCAGCAGCCAGAAGCCACAGCAGCAGCGACTTCAAACCCCAGCAGCATGA
CAGACACCCCTGGTAACCCAGCAGCACCGTAGCCTCTGCCCTGTAAAAATCAGACTCGGA
ACCGATGTGTGCTTTAGGGAATTTTAAGTTGCATGCATTTCAGAGACTTTAAGTCAGTTG
GTTTTTATTAGCTGCTTGGTATGCAGTAACTTGGGTGGAGGCAAAACACTAATAAAAGGG
CTAAAAAGGAAAATGATGCTTTTCTTCTATATTCTTACTCTGTACAAATAAAGAAGTTGC
TTGTTGTTTGAGAAGTTTAACCCCGTTGCTTGTTGTTCTGCAGCCCTGTCTACTTGGGCA
CCCCCACCACCTGTTAGCTGTGGTTGTGCACTGTCTTTTGTAGCTCTGGACTGGAGGGGT
AGATGGGGAGTCAATTACCCATCACATAAATATGAAACATTTATCAGAAATGTTGCCATT
TTAATGAGATGATTTTCTTCATCTCATAATTAAAATACCTGACTTTAGAGAGAGTAAAAT
GTGCCAGGAGCCATAGGAATATCTGTATGTTGGATGACTTTAATGCTACATTTTAAAAAA
AGAAAATAAAGTAATAATATAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAA
>OriGene 5' read for NM_004281.3 unedited
GCCCATTGTATACGACTCCTATAGGGCGGGAACAAATCGGCACCCAGGGGGCCGCGGCCA
ACTTCACAGGACTGGACCAGAAGTTTCTAGCCGGCCAGTTGCTACCTCCCTTTATCTCCT
CCTTCCCCTCTGGCAGCGAGGAGGCTATTTCCAGACACTTCCACCCCTCTCTGGCCACGT
CACCCCCGCCTTTAATTCATAAAGGTGCCCGGCGCCGGCTTCCCGGACACGTCGGCGGCG
GAGAGGGGCCCACGGCGGCGGCCCGGCCAGAGACTCGGCGCCCGGAGCCAGCGCCCCGCA
CCCGCGCCCCAGCGGGCAGACCCCAACCCAGCATGAGCGCCGCCACCCACTCGCCCATGA
TGCAGGTGGCGTCCGGCAACGGTGACCGCGACCCTTTGCCCCCCGGATGGGAGATCAAGA
TCGACCCGCAGACCGGCTGGCCCTTCTTCGTGGACCACAACAGCCGCACCACTACGTGGA
ACGACCCGCGCGTGCCCTCTGAGGGCCCCAAGGAGACTCCATCCTCTGCCAATGGCCCTT
CCCGGGAGGGCTCTAGGCTGCCGCCTGCTAGGGAAGGCCACCCTGTGTACCCCCAGCTCC
GACCAGGCTACATTCCCATTCCTGTGCTCCATGAAGCGCTGAGAACCGGCAGGTGCACCC
TTTCCATGTCTATCCCCAGCTCTGGGATGCAGCGATTCCGAACTGAGGCGGCAGCAGCGG
CTCCTCAAAGGTCCCAGTCCACCTCTGCGGGGCATGCCAGAACCACTCCAGCCAGAATAA
CACGTGGAGAACAGGTGGCAGCCGCGCGGCAGGCCAGCGCCCAGGCCTCCCCACGAACCT
GGACCGGTCCCAGTCTTCAAGCTGCCTTGGACTGGCTTATTCCTTATCCTCTCTCTGGCA
CAGCCTGCCTTTCCTCCGGGCAGGGACAGCCCTGGGCGAGTCACATAGCTACGGCGGGGG
GTGACACTATCTTGCCTGTCTGCGTTGATATCACCGTCTGTCGTAGATAAAGTGTATACT
GCAGAGCTCACACATCCACGA
Restriction Sites Please inquire     
ACCN NM_004281
ORF Size 1728 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Reference Data
RefSeq NM_004281.3, NP_004272.2
RefSeq Size 2608
RefSeq ORF 1728
Locus ID 9531
Domains WW, BAG
Protein Families Druggable Genome
Gene Summary BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008]

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