TATA binding protein (TBP) (NM_003194) Human Untagged Clone

CAT#: SC118124

TBP (untagged)-Human TATA box binding protein (TBP), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TBP
• Synonyms: GTF2D; GTF2D1; HDL4; SCA17; TFIID
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118124 sequence for NM_003194 edited (data generated by NextGen Sequencing)
  ATGGATCAGAACAACAGCCTGCCACCTTACGCTCAGGGCTTGGCCTCCCCTCAGGGTGCC
  ATGACTCCCGGAATCCCTATCTTTAGTCCAATGATGCCTTATGGCACTGGACTGACCCCA
  CAGCCTATTCAGAACACCAATAGTCTGTCTATTTTGGAAGAGCAACAAAGGCAGCAGCAG
  CAACAACAACAGCAGCAGCAGCAGCAGCAGCAGCAACAGCAACAGCAGCAGCAGCAGCAG
  CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAACAGGCAGTGGCAGCTGCA
  GCCGTTCAGCAGTCAACGTCCCAGCAGGCAACACAGGGAACCTCAGGCCAGGCACCACAG
  CTCTTCCACTCACAGACTCTCACAACTGCACCCTTGCCGGGCACCACTCCACTGTATCCC
  TCCCCCATGACTCCCATGACCCCCATCACTCCTGCCACGCCAGCTTCGGAGAGTTCTGGG
  ATTGTACCGCAGCTGCAAAATATTGTATCCACAGTGAATCTTGGTTGTAAACTTGACCTA
  AAGACCATTGCACTTCGTGCCCGAAACGCCGAATATAATCCCAAGCGGTTTGCTGCGGTA
  ATCATGAGGATAAGAGAGCCACGAACCACGGCACTGATTTTCAGTTCTGGGAAAATGGTG
  TGCACAGGAGCCAAGAGTGAAGAACAGTCCAGACTGGCAGCAAGAAAATATGCTAGAGTT
  GTACAGAAGTTGGGTTTTCCAGCTAAGTTCTTGGACTTCAAGATTCAGAATATGGTGGGG
  AGCTGTGATGTGAAGTTTCCTATAAGGTTAGAAGGCCTTGTGCTCACCCACCAACAATTT
  AGTAGTTATGAGCCAGAGTTATTTCCTGGTTTAATCTACAGAATGATCAAACCCAGAATT
  GTTCTCCTTATTTTTGTTTCTGGAAAAGTTGTATTAACAGGTGCTAAAGTCAGAGCAGAA
  ATTTATGAAGCATTTGAAAACATCTACCCTATTCTAAAGGGATTCAGGAAGACGACGTAA
  
  Clone variation with respect to NM_003194.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003194 unedited
  ATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCGCCTGGGCC
  GCCGGCTGTTTAACTTCGCTTCCGCTGGCCCATAGTGATCTTTGCAGTGACCCAGCAGCA
  TCACTGTTTCTTGGCGTGTGAAGATAACCCAAGGAATTGAGGAAGTTGCTGAGAAGAGTG
  TGCTGGAGATGCTCTAGGAAAAAATTGAATAGTGAGACGAGTTCCAGCGCAAGGGTTTCT
  GGTTTGCCAAGAAGAAAGTGAACATCATGGATCAGAACAACAGCCTGCCACCTTACGCTC
  AGGGCTTGGCCTCCCCTCAGGGTGCCATGACTCCCGGAATCCCTATCTTTAGTCCAATGA
  TGCCTTATGGCACTGGACTGACCCCACAGCCTATTCAGAACACCAATAGTCTGTCTATTT
  TGGAAGAGCAACAAAGGCAGCAGCAGCAACAACAACAGCAGCAGCAGCAGCAGCAGCAGC
  AGCAACAGCAACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGC
  AGCAGCAACAGGCAGTGGCAGCTGCAGCCGTTCAGCAGTCAACGTCCCAGCAGGCAACAC
  AGGGAACCTCAGGCCAGGCACCACAGCTCTTCCACTCACAGACTCTCACAACTGCACCCT
  TGCCGGGCACCCACTCCACTGTATCCCTCCCCCATGACTCCCATGACCCCCATCACTCCT
  GCCACGCCAGCTTTGGAGAGTTCTGGGATTGTACCGCAGCTGCAAATATTGTATCCACAG
  TGAATCTTGGGTGTAACTTGACCTAAAGACCTTGCACTTCGTGCCCGACCGCCGATTATA
  TCCCAGCGGGTGGCTGCGGTATCATGAGGAATAGAAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003194 unedited
  TCTTGGACCCGGCCGCTATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATAAACTGCATT
  TATTGTACAGAGTACTCCTGAAGAAAGAAAAAAATATGTACAGCCATTCATTTTCATTAA
  CACATATATTCTGTCCTGCAATACTGGAGAGGTGGAATGTGTCTGGCACAGAAATAACCC
  CTAGGAGTTATAAATTAGAAAAACAACACTTTTAAAAAAGATTTTTACTTTTCTGGTAGA
  AATATAAAAACTGTGGTTCATGGGGAAAAACATTAAAATTAAAAAGTCCAATCAATTATA
  GAGGTGGCTTTAACACTTAAAGTTTTCTCCCTCAAACCAACTTGTCAACAGCAGTGTTTA
  AAATCTACATATAAATAAATGGGCAGCGCTGCCCAGATAGCAGCACGGTATGAGCAACTC
  ACAGTCACGCTGCGCGGTGTTCTCAGTGCACAAATAATGCCCCTTCCCGGCATCCCGCGG
  TGGGCACTTACAGAAGGGCATCACCTGGTGCCACACCCTGCAACTCAACATCCATCTTCT
  CACAACACCACCATTTAAAGGTACCAAAACAAACTGATTTGTCTAAAAAAAAAAACACAA
  GGGGGTGGGGGAGGCATGGGTACTTGAAAGCCCTTACTTCGTCTTCCTGAATCCCTATAA
  ATATGGTAGATGCTCCCAAATGCTCACAAATTCCTGCCCTGACTTTTACACCTGGTAATA
  CAACTTTATCCGAAACCCAACTCTCGCACAACCATTCTGGGTTTGATCCTTCCTGCACAT
  TATACCCAGAAAATACCGTCGGCCACATACCACCTACTATGCGCTTGGGTGTACCCCCTG
  CACTATTCACCCTTATGAGCATCCCCCATTTCCACTCCCCGCTCTAATCCCCACCTCTGA
  CTCTCTCAACCTCACTGGCAACGCCACTCTCTGTCTACTCTCTCATTTAACCCGTGNACC
  GACCCACCTGTATACCCACTGTCCCTGCGCCTACTATATAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003194
• Insert Size: 1950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003194.3, NP_003185.1
• RefSeq Size: 1867 bp
• RefSeq ORF: 1020 bp
• Locus ID: 6908
• Cytogenetics: 6q27
• Domains: TBP
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Basal transcription factors, Huntington's disease
• Gene Summary: 'Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 25-42, and expansion of the number of repeats to 45-66 increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2016]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).