Squalene Epoxidase (SQLE) (NM_003129) Human Untagged Clone

CAT#: SC118174

SQLE (untagged)-Human squalene epoxidase (SQLE)


  "NM_003129" in other vectors (7)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol SQLE
Synonyms FLJ30795
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC118174 sequence for NM_003129 edited (data generated by NextGen Sequencing)
ATGTGGACTTTTCTGGGCATTGCCACTTTCACCTATTTTTATAAGAAGTTCGGGGACTTC
ATCACTTTGGCCAACAGGGAGGTCCTGTTGTGCGTGCTGGTGTTCCTCTCGCTGGGCCTG
GTGCTCTCCTACCGCTGTCGCCACCGAAACGGGGGTCTCCTCGGGCGCCAGCAGAGCGGC
TCCCAGTTCGCCCTCTTCTCGGATATTCTCTCAGGCCTGCCTTTCATTGGCTTCTTCTGG
GCCAAATCCCCCCCTGAATCAGAAAATAAGGAGCAGCTCGAGGCCAGGAGGCGCAGAAAA
GGAACCAATATTTCAGAAACAAGCTTAATAGGAACAGCTGCCTGTACATCAACATCTTCT
CAGAATGACCCAGAAGTTATCATCGTGGGAGCTGGCGTGCTTGGCTCTGCTTTGGCAGCT
GTGCTTTCCAGAGATGGAAGAAAGGTGACAGTCATTGAGAGAGACTTAAAAGAGCCTGAC
AGAATAGTTGGAGAATTCCTGCAGCCGGGTGGTTATCATGTTCTCAAAGACCTTGGTCTT
GGAGATACAGTGGAAGGTCTTGATGCCCAGGTTGTAAATGGTTACATGATTCATGATCAG
GAAAGCAAATCAGAGGTTCAGATTCCTTACCCTCTGTCAGAAAACAATCAAGTGCAGAGT
GGAAGAGCTTTCCATCACGGAAGATTCATCATGAGTCTCCGGAAAGCAGCTATGGCAGAG
CCCAATGCAAAGTTTATTGAAGGTGTTGTGTTACAGTTATTAGAGGAAGATGATGTTGTG
ATGGGAGTTCAGTACAAGGATAAAGAGACTGGAGATATCAAGGAACTCCATGCTCCACTG
ACTGTTGTTGCAGATGGGCTTTTCTCCAAGTTCAGGAAAAGCCTGGTCTCCAATAAAGTT
TCTGTATCATCTCATTTTGTTGGCTTTCTTATGAAGAATGCACCACAGTTTAAAGCAAAT
CATGCTGAACTTATTTTAGCTAACCCGAGTCCAGTTCTCATCTACCAGATTTCATCCAGT
GAAACTCGAGTACTTGTTGACATTAGAGGAGAAATGCCAAGGAATTTAAGAGAATACATG
GTTGAAAAAATTTACCCACAAATACCTGATCACCTGAAAGAACCATTCTTAGAAGCCACT
GACAATTCTCATCTGAGGTCCATGCCAGCAAGCTTCCTTCCTCCTTCATCAGTGAAGAAA
CGAGGTGTTCTTCTTTTGGGAGACGCATNNNATATGAGGCATCCACTTACTGGTGGAGGA
ATGACTGTTGCTTTTAAAGATATAAAACTATGGAGAAAACTGCTAAAGGGTATCCCTGAC
CTTTATGATGATGCAGCTATTTTCGAGGCCAAAAAATCATTTTACTGGGCAAGAAAAACA
TCTCATTCCTTTGTCGTGAATATCCTTGCTCAGGCTCTTTATGAATTATTTTCTGCCACA
GATGATTCCCTGCATCAACTAAGAAAAGCCTGTTTTCTTTATTTCAAACTTGGTGGCGAA
TGTGTTGCGGGTCCTGTTGGGCTGCTTTCTGTATTGTCTCCTAACCCTCTAGTTTTAATT
GGACACTTCTTTGCTGTTGCAATCTATGCCGTGTATTTTTGCTTTAAGTCAGAACCTTGG
ATTACAAAACCTCGAGCCCTTCTCAGTAGTGGTGCTGTATTGTACAAAGCGTGTTCTGTA
ATATTTCCTCTAATTTACTCAGAAATGAAGTATATGGTTCATTAA

Clone variation with respect to NM_003129.3
1229 a=>n;1230 t=>n;1231 a=>n
>OriGene 5' read for NM_003129 unedited
CGTCACCATTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGAATATCTG
GATTTCCTGGGCGAGGAGGAGCGAGTCTGCTCGGGAGCTGTTCCAGCAGGCGATTTTTAA
ATACTGCTTTCTACGCCCTATACAACTTGGCTTCACATACTTTTACACTAACTTTATATG
ATTTTTAAAAACTGGTCTGATCGGACTTCTCGTCCTGGGACACTGTTTACTGGAGTCTGG
CCGGCTCTCCGTGCTCCTCTTGGTACCTCATTTTGGGGAGAACCTTAAACCCACTCGAGC
AGATAATCTCCGCCTTGACCGGTGCCACCAAAGAAGCCTTGGAACCATGTGGACTTTTCT
GGGCATTGCCACTTTCACCTATTTTTATAAGAAGTTCGGGGACTTCATCACTTTGGCCAA
CAGGGAGGTCCTGTTGTGCGTGCTGGTGTTCCTCTCGCTGGGCCTGGTGCTCTCCTACCG
CTGTCGCCACCGAAACGGGGGTCTCCTCGGGCGCCAGCAGAGCGGCTCCCAGTTCGCCCT
CTTCTCGGATATTCTCTCAGGCCTGCCTTTCATTGGCTTCTTCTGGGCCAAATCCCCCCC
TGAATCAGAAAATAAGGAGCAGCTCGAGGCCAGGAGGCGCAGAANAGGAACCAATATTTC
AGAAACAAGCTTAATAGGAACAGCTGCCTGTACATCAACATCTTCTCAGAATGACCCAGA
AGTTATCATCGTGGGAGCTGGCGTGCTTGGCTCTGCTTTGGCAGCTGTGCTTTCCAGAGA
TGGAAGAAAGGTGACAGTCATTGAGAGAGACTTAAAGAGCCTGACAGAATANTNGNAGAA
TTTCTGCAGCCGGGTGGTTATCATGGTCTCAAAGACCTTGGTCTTGNAGAACAGTGGAAG
TCCTGATGCCCAGGTTGTAAT
>OriGene 3' read for NM_003129 unedited
AGAGTCGAGTTTTTTTTTTTTTTTTTTCATTCTTCATTTTATTTGCATGTCCCAACAACT
CATCTCAAATACTAAATTCAAAAGAAAAACTGTAGTTCTCCTCAGCATTAGCACTAATTT
ATGGTAACAATCATTTCTTTTAAATGTCTAACTTATTTAACCCCTTCATTTTAAATTGCA
AATTAAAGCATGTATTTACATATTTATATACAAAAAACTTCAAAAACAAATTAATCCAAA
TCTTGGTCCAAGAGTTTCCACTTTATAAGTGGTATGGTACTATGCTATATATATCCTCTT
CCAAAAGTCTCTTAGGACTTGGTAAGTTCCAAATATTCATTCACAAATGGTTCCCCTTTA
AGCTTAATGAACCATATACTTCATTTCTGAGTAAATTAGAGGAAATATTACAGAACACGC
TTTGTACAATACAGCACCACTACTGAGAAGGGCTCGAGGTTTTGTAATCCAAGGTTCTGA
CTTAAAGCANAAATACACGGCATAGATTGCAACAGCAAAGAAGTGTCCAATTAAAACTAG
AGGGTTAGGAGACATTACAGAAAGCAGCCCACAGGACCCCGCACACATTCGCCACCAAGT
TTGAATAAAGAAAACAGCTTTTCTAGTTGATGCAGGAATCATCTGTTGCAGAAATAATCA
TAAAGAGCCTGACANGGATATCCCGACCAAGGATGAAATGNTTTTCTTGCCAGTAAANTG
ATTTTTGGCCTCAAAAATGCCTGCTTATCATAAAGGTCAGGGATACCCTTTAACAGTTTT
TCCCATAGTTTATTTCTTTAAAACACCAGCCATTCCCCACCAATAAGTGGATGCCTCAAT
TTATTGCGTTCCCAAAAGAAGAACCCCTTGTTTCTTACTTGATAAGGAGAAAGAAACTTC
TGGCATGGACCTCCAATGAAAAGCCAGGGCCTTTAAAAGGGCTTCTCAGGGACAAGATTT
GGGGGAAATTTTTCAACCTGATTCTTTTATTCCTTGGCTTTCCCTTAAGCCACAATCTCC
GTTACTGATAAATTCGGAAGAAACTGCCTGGTACTAAAAACCACCTG
Restriction Sites NotI-NotI     
ACCN NM_003129
Insert Size 2500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003129.3, NP_003120.2
RefSeq Size 2989 bp
RefSeq ORF 1725 bp
Locus ID 6713
Cytogenetics 8q24.13
Domains Monooxygenase
Protein Families Druggable Genome, Transmembrane
Protein Pathways Metabolic pathways, Steroid biosynthesis
Gene Summary 'Squalene epoxidase catalyzes the first oxygenation step in sterol biosynthesis and is thought to be one of the rate-limiting enzymes in this pathway. [provided by RefSeq, Jul 2008]'

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