Squalene Epoxidase (SQLE) (NM_003129) Human Untagged Clone

CAT#: SC324651

SQLE (untagged)-Human squalene epoxidase (SQLE)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SQLE
• Synonyms: FLJ30795
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003129.3 AAACTGGTCTGATCGGACTTCTCGTCCTGGGACACTGTTTACTGGAGTCTGGCCGGCTCT
  CCGTGCTCCTCTTGGTACCTCATTTTGGGGAGAACCTTAAACCCACTCGAGCAGATAATC
  TCCGCCTTGACCGGTGCCACCAAAGAAGCCTTGGAACCATGTGGACTTTTCTGGGCATTG
  CCACTTTCACCTATTTTTATAAGAAGTTCGGGGACTTCATCACTTTGGCCAACAGGGAGG
  TCCTGTTGTGCGTGCTGGTGTTCCTCTCGCTGGGCCTGGTGCTCTCCTACCGCTGTCGCC
  ACCGAAACGGGGGTCTCCTCGGGCGCCAGCAGAGCGGCTCCCAGTTCGCCCTCTTCTCGG
  ATATTCTCTCAGGCCTGCCTTTCATTGGCTTCTTCTGGGCCAAATCCCCCCCTGAATCAG
  AAAATAAGGAGCAGCTCGAGGCCAGGAGGCGCAGAAAAGGAACCAATATTTCAGAAACAA
  GCTTAATAGGAACAGCTGCCTGTACATCAACATCTTCTCAGAATGACCCAGAAGTTATCA
  TCGTGGGAGCTGGCGTGCTTGGCTCTGCTTTGGCAGCTGTGCTTTCCAGAGATGGAAGAA
  AGGTGACAGTCATTGAGAGAGACTTAAAAGAGCCTGACAGAATAGTTGGAGAATTCCTGC
  AGCCGGGTGGTTATCATGTTCTCAAAGACCTTGGTCTTGGAGATACAGTGGAAGGTCTTG
  ATGCCCAGGTTGTAAATGGTTACATGATTCATGATCAGGAAAGCAAATCAGAGGTTCAGA
  TTCCTTACCCTCTGTCAGAAAACAATCAAGTGCAGAGTGGAAGAGCTTTCCATCACGGAA
  GATTCATCATGAGTCTCCGGAAAGCAGCTATGGCAGAGCCCAATGCAAAGTTTATTGAAG
  GTGTTGTGTTACAGTTATTAGAGGAAGATGATGTTGTGATGGGAGTTCAGTACAAGGATA
  AAGAGACTGGAGATATCAAGGAACTCCATGCTCCACTGACTGTTGTTGCAGATGGGCTTT
  TCTCCAAGTTCAGGAAAAGCCTGGTCTCCAATAAAGTTTCTGTATCATCTCATTTTGTTG
  GCTTTCTTATGAAGAATGCACCACAGTTTAAAGCAAATCATGCTGAACTTATTTTAGCTA
  ACCCGAGTCCAGTTCTCATCTACCAGATTTCATCCAGTGAAACTCGAGTACTTGTTGACA
  TTAGAGGAGAAATGCCAAGGAATTTAAGAGAATACATGGTTGAAAAAATTTACCCACAAA
  TACCTGATCACCTGAAAGAACCATTCTTAGAAGCCACTGACAATTCTCATCTGAGGTCCA
  TGCCAGCAAGCTTCCTTCCTCCTTCATCAGTGAAGAAACGAGGTGTTCTTCTTTTGGGAG
  ACGCATATAATATGAGGCATCCACTTACTGGTGGAGGAATGACTGTTGCTTTTAAAGATA
  TAAAACTATGGAGAAAACTGCTAAAGGGTATCCCTGACCTTTATGATGATGCAGCTATTT
  TCGAGGCCAAAAAATCATTTTACTGGGCAAGAAAAACATCTCATTCCTTTGTCGTGAATA
  TCCTTGCTCAGGCTCTTTATGAATTATTTTCTGCCACAGATGATTCCCTGCATCAACTAA
  GAAAAGCCTGTTTTCTTTATTTCAAACTTGGTGGCGAATGTGTTGCGGGTCCTGTTGGGC
  TGCTTTCTGTATTGTCTCCTAACCCTCTAGTTTTAATTGGACACTTCTTTGCTGTTGCAA
  TCTATGCCGTGTATTTTTGCTTTAAGTCAGAACCTTGGATTACAAAACCTCGAGCCCTTC
  TCAGTAGTGGTGCTGTATTGTACAAAGCGTGTTCTGTAATATTTCCTCTAATTTACTCAG
  AAATGAAGTATATGGTTCATTAAGCTTAAAGGGGAACCATTTGTGAATGAATATTTGGAA
  CTTACCAAGTCCTAAGAGACTTTTGGAAGAGGATATATATAGCATAGTACCATACCACTT
  ATAAAGTGGAAACTCTTGGACCAAGATTTGGATTAATTTGTTTTTGAAGTTTTTTGTATA
  TAAATATGTAAATACATGCTTTAATTTGCAATTTAAAATGAAGGGGTTAAATAAGTTAGA
  CATTTAAAAGAAATGATTGTTACCATAAATTAGTGCTAATGCTGAGGAGAACTACAGTTT
  TTCTTTTGAATTTAGTATTTGAGATGAGTTGTTGGGACATGCAAATAAAATGAAGAATGA
  AAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_003129
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003129.3, NP_003120.2
• RefSeq Size: 2989 bp
• RefSeq ORF: 1725 bp
• Locus ID: 6713
• Cytogenetics: 8q24.13
• Domains: Monooxygenase
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, Steroid biosynthesis
• Gene Summary: 'Squalene epoxidase catalyzes the first oxygenation step in sterol biosynthesis and is thought to be one of the rate-limiting enzymes in this pathway. [provided by RefSeq, Jul 2008]'