Fascin (FSCN1) (NM_003088) Human Untagged Clone

CAT#: SC118222

FSCN1 (untagged)-Human fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus) (FSCN1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FSCN1
• Synonyms: FAN1; HSN; p55; SNL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118222 sequence for NM_003088 edited (data generated by NextGen Sequencing)
  ATGACCGCCAACGGCACAGCCGAGGCGGTGCAGATCCAGTTCGGCCTCATCAACTGCGGC
  AACAAGTACCTGACGGCCGAGGCGTTCGGGTTCAAGGTGAACGCGTCCGCCAGCAGCCTG
  AAGAAGAAGCAGATCTGGACGCTGGAGCAGCCCCCTGACGAGGCGGGCAGCGCGGCCGTG
  TGCCTGCGCAGCCACCTGGGCCGCTACCTGGCGGCGGACAAGGACGGCAACGTGACCTGC
  GAGCGCGAGGTGCCCGGTCCCGACTGCCGTTTCCTCATCGTGGCGCACGACGACGGTCGC
  TGGTCGCTGCAGTCCGAGGCGCACCGGCGCTACTTCGGCGGCACCGAGGACCGCCTGTCC
  TGCTTCGCGCAGACGGTGTCCCCCGCCGAGAAGTGGAGCGTGCACATCGCCATGCACCCT
  CAGGTCAACATCTACAGCGTCACCCGTAAGCGCTACGCGCACCTGAGCGCGCGGCCGGCC
  GACGAGATCGCCGTGGACCGCGACGTGCCCTGGGGCGTCGACTCGCTCATCACCCTCGCC
  TTCCAGGACCAGCGCTACAGCGTGCAGACCGCCGACCACCGCTTCCTGCGCCACGACGGA
  CGCCTGGTGGCGCGCCCCGAGCCGGCCACTGGCTACACGCTGGAGTTCCGCTCCGGCAAG
  GTGGCCTTCCGCGACTGCGAGGGCCGTTACCTGGCGCCGTCGGGGCCCAGCGGCACGCTC
  AAGGCGGGCAAGGCCACCAAGGTGGGCAAGGACGAGCTCTTTGCTCTGGAGCAGAGCTGC
  GCCCAGGTCGTGCTGCAGGCGGCCAACGAGAGGAACGTGTCCACGCGCCAGGGTATGGAC
  CTGTCTGCCAATCAGGACGAGGAGACCGACCAGGAGACCTTCCAGCTGGAGATCGACCGC
  GACACCAAAAAGTGTGCCTTCCGTACCCACACGGGCAAGTACTGGACGCTGACGGCCACC
  GGGGGCGTGCAGTCCACCGCCTCCAGCAAGAATGCCAGCTGCTACTTTGACATCGAGTGG
  CGTGACCGGCGCATCACACTGAGGGCGTCCAATGGCAAGTTTGTGACCTCCAAGAAGAAT
  GGGCAGCTGGCCGCCTCGGTGGAGACAGCAGGGGACTCAGAGCTCTTCCTCATGAAGCTC
  ATCAACCGCCCCATCATCGTGTTCCGCGGGGAGCATGGCTTCATCGGCTGCCGCAAGGTC
  ACGGGCACCCTGGACGCCAACCGCTCCAGCTATGACGTCTTCCAGCTGGAGTTCAACGAT
  GGCGCCTACAACATCAAAGACTCCACAGGCAAATACTGGACGGTGGGCAGTGACTCCGCG
  GTCACCAGCAGCGGCGACACTCCTGTGGACTTCTTCTTCGAGTTCTGCGACTATAACAAG
  GTGGCCATCAAGGTGGGCGGGCGCTACCTGAAGGGCGACCACGCAGGCGTCCTGAAGGCC
  TCGGCGGAAACCGTGGACCCCGCCTCGCTCTGGGAGTACTAG
  
  Clone variation with respect to NM_003088.3
  600 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003088 unedited
  ATACGACTTACTATAGGCGGCCGCGATTCGGCACGAGGGCGGAGGGTGCGTGCGGGCCGC
  GGCAGCCGAACAAAGGAGCAGGGGCGCCGCCGCAGGGACCCGCCACCCACCTCCCGGGGC
  CGCGCAGCGGCCTCTCGTCTACTGCCACCATGACCGCCAACGGCACAGCCGAGGCGGTGC
  AGATCCAGTTCGGCCTCATCAACTGCGGCAACAAGTACCTGACGGCCGAGGCGTTCGGGT
  TCAAGGTGAACGCGTCCGCCAGCAGCCTGAAGAAGAAGCAGATCTGGACGCTGGAGCAGC
  CCCCTGACGAGGCGGGCAGCGCGGCCGTGTGCCTGCGCAGCCACCTGGGCCGCTACCTGG
  CGGCGGACAAGGACGGCAACGTGACCTGCGAGCGCGAGGTGCCCGGTCCCGACTGCCGTT
  TCCTCATCGTGGCGCACGACGACGGTCGCTGGTCGCTGCAGTCCGAGGCGCACCGGCGCT
  ACTTCGGCGGCACCGAGGACCGCCTGTCCTGCTTCGCGCAGACGGTGTCCCCCGCCGAGA
  AGTGGAGCGTGCACATCGCCATGCACCCTCAGGTCAACATCTACAGCGTCACCCGTAAGC
  GCTACGCGCACCTGAGCGCGCGGCCGGCCGACGAGATCGCCGTGGACCGCGACGTGCCCT
  GGGGCGTCGACTCGCTCATCACCCTCGCCTTCCAGGACCAGCGCTACAGCGTGCAGACCG
  NCGACCACCGCTTCCTGCGCCACGACNGACGCCTGGTGGCGCGCCCCGAGCCGGCCACTG
  GCTACACGCTGGAGTNCCCGCTCGGCAGGGTGGNCTTCGCGACTGCGAGGGCCGNTACCT
  GNCGCCCGTCGGGCCCAGCGCACGCTCAAGCGGGCAGGNCACCAGGTGGCAGGACAGCTC
  TTGCTCTGACAAACTGCCCCCAGTCTGCTGCAGCGGCACGANAGAACGTGCCCCGCCAGG
  GTATGACTGCTGCATCAGACAGGGACGACCAGAACTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003088 unedited
  TGCACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCAGACTGAGTTA
  TTTTATTTCGCTATTTCCAGTTTGAAGCTACTATCATGGGCGTTTAGAGTTATACAAATG
  ACACTTACAAAAAATAAAAGACCAAGACACCCAGAGTGAGATGCATGTTGGGGACGGGGG
  AGGCTGGCAGCAGGGGGGCCCCGGCGGCTCACCCCAGGGCTCCCGGAGGGGGCGACGCCT
  GGCTTCATCCACCCGGGAGGCCCAGGGAGCACCAATCACAGCAGGGGCTCTGGCCCAGGT
  GTCGGCAGCCCAGGCCCAGGGCTGGGGCTGGACGGGAAGGACGGAAAGAGGGGGCTGAGA
  TGCACCCCCTGGGGAGGGTGCTGAGACGCCCCCCGCCAGATCACTCGCTACTACAGCCAG
  GCTTGCCTGGGACGCCTCCAGCAATAATATTTCATTAAAAAAAATACTGATTTGGTCATT
  TTCTGCTTCCAGTCAGGCTAGGGTGAAAGGAAAGAGACAGGAGGAAGACGGTCCCCACAC
  CCCTACCCGGCCGACACCACCACCTTGGCAGAACGTGCAGCGGGCAGACCCTGTGGCCGA
  CACGCCCTGCCGCTCCAGGGAAGGGAGCCAGGTTGAGCCTTTGACACGTGGGAAAAGGGC
  TGTACCCCCCCACCCCCCAATAAACCCCACAGGGGAAAAACCTAACCACCCAACAGTTTA
  CTTTACCAAAAGGGGATTTGGGGCCACTGCCCCACGGAAGGCCATTAAAGGGCTTGGAAA
  TTGGCACAAATTTATTTGGGTGCTCGGAAAGAAGGCACCTTCCCACTCCTCCTTTTCCGC
  CCCTCCTTTCATCCACTTTCTTCCCCCCCCCCACATTTATCCTCATCTCACCACAGGTAC
  G
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003088
• Insert Size: 2930 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003088.2, NP_003079.1
• RefSeq Size: 2819 bp
• RefSeq ORF: 1482 bp
• Locus ID: 6624
• Cytogenetics: 7p22.1
• Gene Summary: 'This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15. [provided by RefSeq, Sep 2011]'