Fascin (FSCN1) (NM_003088) Human Untagged Clone

CAT#: SC320744

FSCN1 (untagged)-Human fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus) (FSCN1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FSCN1
• Synonyms: FAN1; HSN; p55; SNL
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003088.2 GCTGCGGAGGGTGCGTGCGGGCCGCGGCAGCCGAACAAAGGAGCAGGGGCGCCGCCGCAG
  GGACCCGCCACCCACCTCCCGGGGCCGCGCAGCGGCCTCTCGTCTACTGCCACCATGACC
  GCCAACGGCACAGCCGAGGCGGTGCAGATCCAGTTCGGCCTCATCAACTGCGGCAACAAG
  TACCTGACGGCCGAGGCGTTCGGGTTCAAGGTGAACGCGTCCGCCAGCAGCCTGAAGAAG
  AAGCAGATCTGGACGCTGGAGCAGCCCCCTGACGAGGCGGGCAGCGCGGCCGTGTGCCTG
  CGCAGCCACCTGGGCCGCTACCTGGCGGCGGACAAGGACGGCAACGTGACCTGCGAGCGC
  GAGGTGCCCGGTCCCGACTGCCGTTTCCTCATCGTGGCGCACGACGACGGTCGCTGGTCG
  CTGCAGTCCGAGGCGCACCGGCGCTACTTCGGCGGCACCGAGGACCGCCTGTCCTGCTTC
  GCGCAGACGGTGTCCCCCGCCGAGAAGTGGAGCGTGCACATCGCCATGCACCCTCAGGTC
  AACATCTACAGCGTCACCCGTAAGCGCTACGCGCACCTGAGCGCGCGGCCGGCCGACGAG
  ATCGCCGTGGACCGCGACGTGCCCTGGGGCGTCGACTCGCTCATCACCCTCGCCTTCCAG
  GACCAGCGCTACAGCGTGCAGACCGCCGACCACCGCTTCCTGCGCCACGACGGGCGCCTG
  GTGGCGCGCCCCGAGCCGGCCACTGGCTACACGCTGGAGTTCCGCTCCGGCAAGGTGGCC
  TTCCGCGACTGCGAGGGCCGTTACCTGGCGCCGTCGGGGCCCAGCGGCACGCTCAAGGCG
  GGCAAGGCCACCAAGGTGGGCAAGGACGAGCTCTTTGCTCTGGAGCAGAGCTGCGCCCAG
  GTCGTGCTGCAGGCGGCCAACGAGAGGAACGTGTCCACGCGCCAGGGTATGGACCTGTCT
  GCCAATCAGGACGAGGAGACCGACCAGGAGACCTTCCAGCTGGAGATCGACCGCGACACC
  AAAAAGTGTGCCTTCCGTACCCACACGGGCAAGTACTGGACGCTGACGGCCACCGGGGGC
  GTGCAGTCCACCGCCTCCAGCAAGAATGCCAGCTGCTACTTTGACATCGAGTGGCGTGAC
  CGGCGCATCACACTGAGGGCGTCCAATGGCAAGTTTGTGACCTCCAAGAAGAATGGGCAG
  CTGGCCGCCTCGGTGGAGACAGCAGGGGACTCAGAGCTCTTCCTCATGAAGCTCATCAAC
  CGCCCCATCATCGTGTTCCGCGGGGAGCATGGCTTCATCGGCTGCCGCAAGGTCACGGGC
  ACCCTGGACGCCAACCGCTCCAGCTATGACGTCTTCCAGCTGGAGTTCAACGATGGCGCC
  TACAACATCAAAGACTCCACAGGCAAATACTGGACGGTGGGCAGTGACTCCGTGGTCACC
  AGCAGCGGCGACACTCCTGTGGACTTCTTCTTCGAGTTCTGCGACTATAACAAGGTGGCC
  ATCAAGGTGGGCGGGCGCTACCTGAAGGGCGACCACGCAGGCGTCCTGAAGGCCTCGGCG
  GAAACCGTGGACCCCGCCTCGCTCTGGGAGTACTAGGGCCGGCCCGTCCTTCCCCGCCCC
  TGCCCACATGGCGGCTCCTGCCAACCCTCCCTGCTAACCCCTTCTCCGCCAGGTGGGCTC
  CAGGGCGGGAGGCAAGCCCCCTTGCCTTTCAAACTGGAAACCCCAGAGAAAACGGTGCCC
  CCACCTGTCGCCCCTATGGACTCCCCACTCTCCCCTCCGCCCGGGTTCCCTACTCCCCTC
  GGGTCAGCGGCTGCGGCCTGGCCCTGGGAGGGATTTCAGATGCCCCTGCCCTCTTGTCTG
  CCACGGGGCGAGTCTGGCACCTCTTTCTTCTGACCTCAGACGGCTCTGAGCCTTATTTCT
  CTGGAAGCGGCTAAGGGACGGTTGGGGGCTGGGAGCCCTGGGCGTGTAGTGTAACTGGAA
  TCTTTTGCCTCTCCCAGCCACCTCCTCCCAGCCCCCCAGGAGAGCTGGGCACATGTCCCA
  AGCCTGTCAGTGGCCCTCCCTGGTGCACTGTCCCCGAAACCCCTGCTTGGGAAGGGAAGC
  TGTCGGGTGGGCTAGGACTGACCCTTGTGGTGTTTTTTTGGGTGGTGGCTGGAAACAGCC
  CCTCTCCCACGTGGCAGAGGCTCAGCCTGGCTCCCTTCCCTGGAGCGGCAGGGCGTGACG
  GCCACAGGGTCTGCCCGCTGCACGTTCTGCCAAGGTGGTGGTGGCGGGCGGGTAGGGGTG
  TGGGGGCCGTCTTCCTCCTGTCTCTTTCCTTTCACCCTAGCCTGACTGGAAGCAGAAAAT
  GACCAAATCAGTATTTTTTTTAATGAAATATTATTGCTGGAGGCGTCCCAGGCAAGCCTG
  GCTGTAGTAGCGAGTGATCTGGCGGGGGGCGTCTCAGCACCCTCCCCAGGGGGTGCATCT
  CAGCCCCCTCTTTCCGTCCTTCCCGTCCAGCCCCAGCCCTGGGCCTGGGCTGCCGACACC
  TGGGCCAGAGCCCCTGCTGTGATTGGTGCTCCCTGGGCCTCCCGGGTGGATGAAGCCAGG
  CGTCGCCCCCTCCGGGAGCCCTGGGGTGAGCCGCCGGGGCCCCCCCTGCTGCCAGCCTCC
  CCCGTCCCCAACATGCATCTCACTCTGGGTGTCTTGGTCTTTTATTTTTTGTAAGTGTCA
  TTTGTATAACTCTAAACGCCCATGATAGTAGCTTCAAACTGGAAATAGCGAAATAAAATA
  ACTCAGTCTGCAGCCCCAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_003088
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003088.2, NP_003079.1
• RefSeq Size: 2819 bp
• RefSeq ORF: 1482 bp
• Locus ID: 6624
• Cytogenetics: 7p22.1
• Gene Summary: 'This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15. [provided by RefSeq, Sep 2011]'