Proteasome beta 1 (PSMB1) (NM_002793) Human Untagged Clone

CAT#: SC118415

PSMB1 (untagged)-Human proteasome (prosome, macropain) subunit, beta type, 1 (PSMB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMB1
• Synonyms: HC5; PMSB1; PSC5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118415 sequence for NM_002793 edited (data generated by NextGen Sequencing)
  ATGTTGTCCTCTACAGCCATGTATTCGGCTCCTGGCAGAGACTTGGGGATGGAACCGCAC
  AGAGCCGCGGGCCCTTTGCAGCTGCGATTTTCGCCCTACGTTTTCAACGGAGGTACTATA
  CTGGCAATTGCTGGAGAAGATTTTGCAATTGTTGCTTCTGATACTCGATTGAGTGAAGGG
  TTTTCAATTCATACGCGGGATAGCCCCAAATGTTACAAATTAACAGACAAAACAGTCATT
  GGATGCAGCGGTTTTCATGGAGACTGTCTTACGCTGACAAAGATTATTGAAGCAAGACTA
  AAGATGTATAAGCATTCCAATAATAAGGCCATGACTACGGGGGCAATTGCTGCAATGCTG
  TCTACAATCCTGTATTCAAGGCGCTTCTTTCCATACTATGTTTACAACATCATCGGTGGA
  CTTGATGAAGAAGGAAAGGGGGCTGTATACAGCTTTGATCCAGTAGGGTCTTACCAGAGA
  GACTCCTTCAAGGCTGGAGGCTCAGCAAGTGCCATGCTACAGCCCCTGCTTGACAACCAG
  GTTGGTTTTAAGAACATGCAGAATGTGGAGCATGTTCCGCTGTCCTTGGACAGAGCCATG
  CGGCTGGTGAAAGATGTCTTCATTTCTGCGGCTGAGAGAGATGTGTACACTGGGGACGCA
  CTCCGGATCTGCATAGTGACCAAAGAGGGCATCAGGGAGGAAACTGTTTCCTTAAGGAAG
  GACTGA
  
  Clone variation with respect to NM_002793.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002793 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAAGTGTCGGATCCGCAGG
  CGCAGCCGTGCGATGTTGTCCTCTACAGCCATGTATTCGGCTCCTGGCAGAGACTTGGGG
  ATGGAACCGCACAGAGCCGCGGGCCCTTTGCAGCTGCGATTTTCGCCCTACGTTTTCAAC
  GGAGGTACTATACTGGCAATTGCTGGAGAAGATTTTGCAATTGTTGCTTCTGATACTCGA
  TTGAGTGAAGGGTTTTCAATTCATACGCGGGATAGCCCCAAATGTTACAAATTAACAGAC
  AAAACAGTCATTGGATGCAGCGGTTTTCATGGAGACTGTCTTACGCTGACAAAGATTATT
  GAAGCAAGACTAAAGATGTATAAGCATTCCAATAATAAGGCCATGACTACGGGGGCAATT
  GCTGCAATGCTGTCTACAATCCTGTATTCAAGGCGCTTCTTTCCATACTATGTTTACAAC
  ATCATCGGTGGACTTGATGAAGAAGGAAAGGGGGCTGTATACAGCTTTGATCCAGTAGGG
  TCTTACCAGAGAGACTCCTTCAAGGCTGGAGGCTCAGCAAGTGCCATGCTACAGCCCCTG
  CTTGACAACCAGGTTGGTTNTAAGAACATGCAGAATGTGGAGCATGTTCCGCTGTCCTTG
  GACAGAGCCATGCGGCTGGTGAAAGATGTCTTCATTTCTGCGGCTGAGAGAGATGTGTAC
  ACTGGGGACGCACTCCCNATCTGCATAGTGACCAAAGAGGGCATCAGGNAGGGAAACTGT
  TCCTTAGGNANGACTGATCTGTGTGCTCTTATACCAATCAGTNAGACCTGGNTGATTTGT
  ACTTTGAACTGTACCTGGATGGTTTTGTTATTAAAGAGAAACCTGAGTAAAAAAAAAAAA
  AAACTCACTCTAAATGGGCCGGGTCTAGCTGTTCCTGACGATCGGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002793 unedited
  GGACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTACTTCAGGTTTCTC
  TTTTAATAAACAAAACCATCCAAGGTACAGTTCCAAAGTACAAAATCAACCAGGTCTGAA
  CTGATTGGTGATAAGAGCACACAGATCAGTCCTTCCTTAAGGAAACAGTTTCCTCCCTGA
  TGCCCTCTTTGGTCACTATGCAGATCCGGAGTGCGTCCCCAGTGTACACATCTCTCTCAG
  CCGCAGAAATGAAGACATCTTTCACCAGCCGCATGGCTCTGTCCAAGGACAGCGGAACAT
  GCTCCACATTCTGCATGTTCTTAAAACCAACCTGGTTGTCAAGCAGGGGCTGTAGCATGG
  CACTTGCTGAGCCTCCAGCCTTGAAGGAGTCTCTCTGGTAAGACCCTACTGGATCAAAGC
  TGTATACAGCCCCCTTTCCTTCTTCATCAAGTCCACCGATGATGTTGTAAACATAGTATG
  GAAAGAAGCGCCTTGAATACAGGATTGTAGACAGCATTGCAGCAATTGCCCCCGTACTCA
  TGGCCTTATTATTGGAATGCTTATACATCTTTAGCCTTGCTCAATAATCTTTGGCAGCGA
  AAGACAGTCTCCATGAAAACCGCTGCATCCAAAGACTGTTTTGGCCTGGCAAATTTGGAA
  CCTTTGGGGCTTTCCCCCGTATGAACTGAAAACCCTCCACTCAATCCAGTATCCAAAACA
  ACACTTGCACAACCTTCTTCAGCATTGCCAGNTTAATAACCTTCGTTGAAAACGAAGGGC
  AAAACTCCCATTGCAAAGGGCCCCGGGTTTTTGCGGGTCCTTCCCAATCTTTTCCCGAAC
  CCAATACTGCTGTGAAGGCAACATTCACGGTGGGCCTGGGGATCCGACCTGCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002793
• Insert Size: 860 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002793.2, NP_002784.1
• RefSeq Size: 872 bp
• RefSeq ORF: 726 bp
• Locus ID: 5689
• Cytogenetics: 6q27
• Domains: proteasome
• Protein Families: Protease
• Protein Pathways: Proteasome
• Gene Summary: 'The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is tightly linked to the TBP (TATA-binding protein) gene in human and in mouse, and is transcribed in the opposite orientation in both species. [provided by RefSeq, Jul 2008]'