CCN3 (NOV) (NM_002514) Human Untagged Clone

CAT#: SC118570

NOV (untagged)-Human nephroblastoma overexpressed gene (NOV)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CCN3
• Synonyms: IBP-9; IGFBP-9; IGFBP9; NOV; NOVh
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118570 sequence for NM_002514 edited (data generated by NextGen Sequencing)
  ATGCAGAGTGTGCAGAGCACGAGCTTTTGTCTCCGAAAGCAGTGCCTTTGCCTGACCTTC
  CTGCTTCTCCATCTCCTGGGACAGGTCGCTGCGACTCAGCGCTGCCCTCCCCAGTGCCCG
  GGCCAGTGCCCTGCGACGCCGCCGACCTGCGCCCCCGGGGTGCGCGCGGTGCTGGACGGC
  TGCTCATGCTGTCTGGTGTGTGCCCGCCAGCGTGGCGAGAGCTGCTCAGATCTGGAGCCA
  TGCGACGAGAGCAGTGGCCTCTACTGTGATCGCAGCGCGGACCCCAGCAACCAGACTGGC
  ATCTGCACGGCGGTAGAGGGAGATAACTGTGTGTTCGATGGGGTCATCTACCGCAGTGGA
  GAGAAATTTCAGCCAAGCTGCAAATTCCAGTGCACCTGCAGAGATGGGCAGATTGGCTGT
  GTGCCCCGCTGTCAGCTGGATGTGCTACTGCCTGAGCCTAACTGCCCAGCTCCAAGAAAA
  GTTGAGGTGCCTGGAGAGTGCTGTGAAAAGTGGATCTGTGGCCCAGATGAGGAGGATTCA
  CTGGGAGGCCTTACCCTTGCAGCTTACAGGCCAGAAGCCACCCTAGGAGTAGAAGTCTCT
  GACTCAAGTGTCAACTGCATTGAACAGACCACAGAGTGGACAGCATGCTCCAAGAGCTGT
  GGTATGGGGTTCTCCACCCGGGTCACCAATAGGAACCGTCAATGTGAGATGCTGAAACAG
  ACTCGGCTCTGCATGGTGCGGCCCTGTGAACAAGAGCCAGAGCAGCCAACAGATAAGAAA
  GGAAAAAAGTGTCTCCGCACCAAGAAGTCACTCAAAGCCATCCACCTGCAGTTCAAGAAC
  TGCACCAGCCTGCACACCTACAAGCCCAGGTTCTGTGGGGTCTGCAGTGATGGCCGCTGC
  TGCACTCCCCACAATACCAAAACCATCCAGGCAGAGTTTCAGTGCTCCCCAGGGCAAATA
  GTCAAGAAGCCAGTGATGGTCATTGGGACCTGCACCTGTCACACCAACTGTCCTAAGAAC
  AATGAGGCCTTCCTCCAGGAGCTGGAGCTGAAGACTACCAGAGGGAAAATGTAA
  
  Clone variation with respect to NM_002514.3
  125 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002514 unedited
  GGGTTCGAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCAGCGAAG
  AAGTCTCGTTTGGTAAAAGCGAGAGGGGAAAGCCTGAGCATGCAGAGTGTGCAGAGCACG
  AGCTTTTGTCTCCGAAAGCAGTGCCTTTGCCTGACCTTCCTGCTTCTCCATCTCCTGGGA
  CAGGTCGCTGCGACTCAGCGCTGCCCTCCCCAGTGCCCGGGCCAGTGCCCTGCGACGCCG
  CCGACCTGCGCCCCCGGGGTGCGCGCGGTGCTGGACGGCTGCTCATGCTGTCTGGTGTGT
  GCCCGCCAGCGTGGCGAGAGCTGCTCAGATCTGGAGCCATGCGACGAGAGCAGTGGCCTC
  TACTGTGATCGCAGCGCGGACCCCAGCAACCAGACTGGCATCTGCACGGCGGTAGAGGGA
  GATAACTGTGTGTTCGATGGGGTCATCTACCGCAGTGGAGAGAAATTTCAGCCAAGCTGC
  AAATTCCAGTGCACCTGCAGAGATGGGCAGATTGGCTGTGTGCCCCGCTGTCAGCTGGAT
  GTGCTACTGCCTGAGCCTAACTGCCCAGCTCCAAGAAAAGTTGAGGTGCCTGGAGAGTGC
  TGTGAAAAGTGGATCTGTGGCCCAGATGAGGAGGATTCACTGGGAGGCCTTACCCTTGCA
  GCTTACAGGCCAGAAGCCACCCTAGGAGTAGAAGTCTCTGACTCAAGTGTCAACTGCATT
  GAACAGACCACAGAGTGGACAGCATGCTCCAAGAGCTGTGGTATGGNGTTCTCCACCCGG
  GTCACCAATAGGAACCGTCAATGTGAGATGCTGAAACAGACCTCGCTCTGCATGGTGCGG
  GCCCTGTGAACAAGAGCCCGAGCAGCCAACAGATTAGAAAGGAAAAAGTGTCTTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002514 unedited
  CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAATTCATAAATTTTAATT
  TTATTTTCAGTAAACAACAATGTTCAACTTTTATGCACCAAAGTAATACAGAGTCACGCA
  GCAATAAAATCGTGTTTATTTAGTGATATGACAAGTAGAGAAATCAAACATAATATTTTA
  CAAAATGTACAATTTATATTTTTATAAATACTTTAAAAGAGAGAAGCGGAACACGTGTGG
  TGGCATTAAAAGAATGTCTAGATTTAATTTTGCAGCCTACCTTGTAGCCTGACAAACCAG
  ATACATGTATGTATACACATATATCTTGATCATGAGTTTTCATAATTATGGCCCAAGGAT
  AAGTTTTGGGCATGTCTACAATAATTCCAAACTCTCATAAAATGTGTGGTCTCTCACCAC
  CAGTCATGTTGATTTTGCTCTATTTTTCTCAAAATTCCACAAAATGCACATTTGAAACAG
  CTATCAGAGGATGTAAGCAAGTGCTTTGGGCCTCAGTTTACTCATCAGTTAGATGAGAGA
  ATCCCTGCTAATATGCTGTTTCTGCTTAATTAAAAAACTTGNGGGCCTCAGCAGCTTCCA
  ATATATAAATGTCTGAGGGCTATGAGGGACCAGTCATCTTTGTATTGGNGAGTTTCTGTT
  TAGTTTCTTTCTATTGGAAAGGAGTCAATGTGTCTTAAATGGAGAAAGTTTATGACACCA
  GTNCACTTNAGTAAACATTTCATTACTGTAGATACCACACGTTTTCCTTAGTGTAAAACT
  TAACACGAAAGTTATAGTCATCTCTGGACGTCATGTTGAAAAGCTGCATGTTTCTTGGNA
  TTTGNACCTGGAAGTCAGAGCTGGTTACCCAAAAGGGAGCACTAGAGAATTTTCCCATAA
  CCTAATATATGGTTGNAAACCTGAACATGAATGTNAGTTTGATCTTAACTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002514
• Insert Size: 2680 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002514.2, NP_002505.1
• RefSeq Size: 2389 bp
• RefSeq ORF: 1074 bp
• Locus ID: 4856
• Cytogenetics: 8q24.12
• Domains: IB, tsp_1, VWC, CT, Cys_knot
• Protein Families: ES Cell Differentiation/IPS, Secreted Protein
• Gene Summary: 'The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development. [provided by RefSeq, Feb 2009]'