CCN3 (NOV) (NM_002514) Human Untagged Clone

CAT#: SC118570

NOV (untagged)-Human nephroblastoma overexpressed gene (NOV)


  "NM_002514" in other vectors (7)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CCN3
Synonyms IBP-9; IGFBP-9; IGFBP9; NOV; NOVh
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC118570 sequence for NM_002514 edited (data generated by NextGen Sequencing)
ATGCAGAGTGTGCAGAGCACGAGCTTTTGTCTCCGAAAGCAGTGCCTTTGCCTGACCTTC
CTGCTTCTCCATCTCCTGGGACAGGTCGCTGCGACTCAGCGCTGCCCTCCCCAGTGCCCG
GGCCAGTGCCCTGCGACGCCGCCGACCTGCGCCCCCGGGGTGCGCGCGGTGCTGGACGGC
TGCTCATGCTGTCTGGTGTGTGCCCGCCAGCGTGGCGAGAGCTGCTCAGATCTGGAGCCA
TGCGACGAGAGCAGTGGCCTCTACTGTGATCGCAGCGCGGACCCCAGCAACCAGACTGGC
ATCTGCACGGCGGTAGAGGGAGATAACTGTGTGTTCGATGGGGTCATCTACCGCAGTGGA
GAGAAATTTCAGCCAAGCTGCAAATTCCAGTGCACCTGCAGAGATGGGCAGATTGGCTGT
GTGCCCCGCTGTCAGCTGGATGTGCTACTGCCTGAGCCTAACTGCCCAGCTCCAAGAAAA
GTTGAGGTGCCTGGAGAGTGCTGTGAAAAGTGGATCTGTGGCCCAGATGAGGAGGATTCA
CTGGGAGGCCTTACCCTTGCAGCTTACAGGCCAGAAGCCACCCTAGGAGTAGAAGTCTCT
GACTCAAGTGTCAACTGCATTGAACAGACCACAGAGTGGACAGCATGCTCCAAGAGCTGT
GGTATGGGGTTCTCCACCCGGGTCACCAATAGGAACCGTCAATGTGAGATGCTGAAACAG
ACTCGGCTCTGCATGGTGCGGCCCTGTGAACAAGAGCCAGAGCAGCCAACAGATAAGAAA
GGAAAAAAGTGTCTCCGCACCAAGAAGTCACTCAAAGCCATCCACCTGCAGTTCAAGAAC
TGCACCAGCCTGCACACCTACAAGCCCAGGTTCTGTGGGGTCTGCAGTGATGGCCGCTGC
TGCACTCCCCACAATACCAAAACCATCCAGGCAGAGTTTCAGTGCTCCCCAGGGCAAATA
GTCAAGAAGCCAGTGATGGTCATTGGGACCTGCACCTGTCACACCAACTGTCCTAAGAAC
AATGAGGCCTTCCTCCAGGAGCTGGAGCTGAAGACTACCAGAGGGAAAATGTAA

Clone variation with respect to NM_002514.3
125 g=>a
>OriGene 5' read for NM_002514 unedited
GGGTTCGAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCAGCGAAG
AAGTCTCGTTTGGTAAAAGCGAGAGGGGAAAGCCTGAGCATGCAGAGTGTGCAGAGCACG
AGCTTTTGTCTCCGAAAGCAGTGCCTTTGCCTGACCTTCCTGCTTCTCCATCTCCTGGGA
CAGGTCGCTGCGACTCAGCGCTGCCCTCCCCAGTGCCCGGGCCAGTGCCCTGCGACGCCG
CCGACCTGCGCCCCCGGGGTGCGCGCGGTGCTGGACGGCTGCTCATGCTGTCTGGTGTGT
GCCCGCCAGCGTGGCGAGAGCTGCTCAGATCTGGAGCCATGCGACGAGAGCAGTGGCCTC
TACTGTGATCGCAGCGCGGACCCCAGCAACCAGACTGGCATCTGCACGGCGGTAGAGGGA
GATAACTGTGTGTTCGATGGGGTCATCTACCGCAGTGGAGAGAAATTTCAGCCAAGCTGC
AAATTCCAGTGCACCTGCAGAGATGGGCAGATTGGCTGTGTGCCCCGCTGTCAGCTGGAT
GTGCTACTGCCTGAGCCTAACTGCCCAGCTCCAAGAAAAGTTGAGGTGCCTGGAGAGTGC
TGTGAAAAGTGGATCTGTGGCCCAGATGAGGAGGATTCACTGGGAGGCCTTACCCTTGCA
GCTTACAGGCCAGAAGCCACCCTAGGAGTAGAAGTCTCTGACTCAAGTGTCAACTGCATT
GAACAGACCACAGAGTGGACAGCATGCTCCAAGAGCTGTGGTATGGNGTTCTCCACCCGG
GTCACCAATAGGAACCGTCAATGTGAGATGCTGAAACAGACCTCGCTCTGCATGGTGCGG
GCCCTGTGAACAAGAGCCCGAGCAGCCAACAGATTAGAAAGGAAAAAGTGTCTTC
>OriGene 3' read for NM_002514 unedited
CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAATTCATAAATTTTAATT
TTATTTTCAGTAAACAACAATGTTCAACTTTTATGCACCAAAGTAATACAGAGTCACGCA
GCAATAAAATCGTGTTTATTTAGTGATATGACAAGTAGAGAAATCAAACATAATATTTTA
CAAAATGTACAATTTATATTTTTATAAATACTTTAAAAGAGAGAAGCGGAACACGTGTGG
TGGCATTAAAAGAATGTCTAGATTTAATTTTGCAGCCTACCTTGTAGCCTGACAAACCAG
ATACATGTATGTATACACATATATCTTGATCATGAGTTTTCATAATTATGGCCCAAGGAT
AAGTTTTGGGCATGTCTACAATAATTCCAAACTCTCATAAAATGTGTGGTCTCTCACCAC
CAGTCATGTTGATTTTGCTCTATTTTTCTCAAAATTCCACAAAATGCACATTTGAAACAG
CTATCAGAGGATGTAAGCAAGTGCTTTGGGCCTCAGTTTACTCATCAGTTAGATGAGAGA
ATCCCTGCTAATATGCTGTTTCTGCTTAATTAAAAAACTTGNGGGCCTCAGCAGCTTCCA
ATATATAAATGTCTGAGGGCTATGAGGGACCAGTCATCTTTGTATTGGNGAGTTTCTGTT
TAGTTTCTTTCTATTGGAAAGGAGTCAATGTGTCTTAAATGGAGAAAGTTTATGACACCA
GTNCACTTNAGTAAACATTTCATTACTGTAGATACCACACGTTTTCCTTAGTGTAAAACT
TAACACGAAAGTTATAGTCATCTCTGGACGTCATGTTGAAAAGCTGCATGTTTCTTGGNA
TTTGNACCTGGAAGTCAGAGCTGGTTACCCAAAAGGGAGCACTAGAGAATTTTCCCATAA
CCTAATATATGGTTGNAAACCTGAACATGAATGTNAGTTTGATCTTAACTC
Restriction Sites NotI-NotI     
ACCN NM_002514
Insert Size 2680 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002514.2, NP_002505.1
RefSeq Size 2389 bp
RefSeq ORF 1074 bp
Locus ID 4856
Cytogenetics 8q24.12
Domains IB, tsp_1, VWC, CT, Cys_knot
Protein Families ES Cell Differentiation/IPS, Secreted Protein
Gene Summary 'The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development. [provided by RefSeq, Feb 2009]'

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