CCN3 (NOV) (NM_002514) Human Untagged Clone

CAT#: SC322366

NOV (untagged)-Human nephroblastoma overexpressed gene (NOV)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CCN3
• Synonyms: IBP-9; IGFBP-9; IGFBP9; NOV; NOVh
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322366 GGCGAGCAGTGCCAATCTACAGCGAAGAAAGTCTCGTTTGGTAAAAGCGAGAGGGGAAAG
  CCTGAGCATGCAGAGTGTGCAGAGCACGAGCTTTTGTCTCCGAAAGCAGTGCCTTTGCCT
  GACCTTCCTGCTTCTCCATCTCCTGGGACAGGTCGCTGCGACTCAGCGCTGCCCTCCCCA
  GTGCCCGGGCCGGTGCCCTGCGACGCCGCCGACCTGCGCCCCCGGGGTGCGCGCGGTGCT
  GGACGGCTGCTCATGCTGTCTGGTGTGTGCCCGCCAGCGTGGCGAGAGCTGCTCAGATCT
  GGAGCCATGCGACGAGAGCAGTGGCCTCTACTGTGATCGCAGCGCGGACCCCAGCAAACA
  GACTGGCATCTGCACGGCGGTAGAGGGAGATAACTGTGTGTTCGATGGGGTCATCTACCG
  CAGTGGAGAGAAATTTCAGCCAAGCTGCAAATTCCAGTGCACCTGCAGAGATGGGCAGAT
  TGGCTGTGTGCCCCGCTGTCAGCTGGATGTGCTACTGCCTGAGCCTAACTGCCCAGCTCC
  AAGAAAAGTTGAGGTGCCTGGAGAGTGCTGTGAAAAGTGGATCTGTGGCCCAGATGAGGA
  GGATTCACTGGGAGGCCTTACCCTTGCAGCTTACAGGCCAGAAGCCACCCTAGGAGTAGA
  AGTCTCTGACTCAAGTGTCAACTGCATTGAACAGACCACAGAGTGGACAGCATGCTCCAA
  GAGCTGTGGTATGGGGTTCTCCACCCGGGTCACCAATAGGAACCGTCAATGTGAGATGCT
  GAAACAGACTCGGCTCTGCATGGTGCGGCCCTGTGAACAAGAGCCAGAGCAGCCAACAGA
  TAAGAAAGGAAAAAAGTGTCTCCGCACCAAGAAGTCACTCAAAGCCATCCACCTGCAGTT
  CAAGAACTGCACCAGCCTGCACACCTACAAGCCCAGGTTCTGTGGGGTCTGCAGTGATGG
  CCGCTGCTGCACTCCCCACAATACCAAAACCATCCAGGCAGAGTTTCAGTGCTCCCCAGG
  GCAAATAGTCAAGAAGCCAGTGATGGTCATTGGGACCTGCACCTGTCACACCAACTGTCC
  TAAGAACAATGAGGCCTTCCTCCAGGAGCTGGAGCTGAAGACTACCAGAGGGAAAATGTA
  ACCTGTCACTCAAGAAGCACACCTACAGAGCACCTGTAGCTGCTGCGCCACCCACCATCA
  AAGGAATATAAGAAAAGTAATGAAGAATCACGATTTCATCCTTGAATCCTATGTATTTTC
  CTAATGTGATCATATGAGGACCTTTATATCTGTCTTTTATTTAACAAAAAATGTAATTAA
  CTGTAAACTTGGAATCAAGGTAAGCTCAGGATATGGCTTAGGAATGACTTACTTTCCTGT
  GGTTTTATTACAAATGCAAATTTCTATAAATTTAAGAAAACAAGTATATAATTTACTTTG
  TAGACTGTTTCACATTGCACTCATCATATTTTGTTGTGCACTAGTGCAATTCCAAGAAAA
  TATCACTGTAATGAGTCAGTGAAGTCTAGAATCATACTTAACATTTCATTGTACAAGTAT
  TACAACCATATATTGAGGTTCATTGGGAAGATTCTCTATTGGCTCCCTTTTTGGGTAAAC
  CAGCTCTGAACTTCCAAGCTCCAAATCCAAGGAAACATGCAGCTCTTCAACATGACATCC
  AGAGATGACTATTACTTTTCTGTTTAGTTTTACACTAGGAAACGTGTTGTATCTACAGTA
  ATGAAATGTTTACTAAGTGGACTGGTGTCATAAACTTTCTCCATTTAAGACACATTGACT
  CCTTTCCAATAGAAAGAAACTAAACAGAAAACTCCCAATACAAAGATGACTGGTCCCTCA
  TAGCCCTCAGACATTTATATATTGGAAGCTGCTGAGGCCCCCAAGTTTTTTAATTAAGCA
  GAAACAGCATATTAGCAGGGATTCTCTCATCTAACTGATGAGTAAACTGAGGCCCAAAGC
  ACTTGCTTACATCCTCTGATAGCTGTTTCAAATGTGCATTTTGTGGAATTTTGAGAAAAA
  TAGAGCAAAATCAACATGACTGGTGGTGAGAGACCACACATTTTATGAGAGTTTGGAATT
  ATTGTAGACATGCCCAAAACTTATCCTTGGGCCATAATTATGAAAACTCATGATCAAGAT
  ATATGTGTATACATACATGTATCTGGTTTGTCAGGCTACAAGGTAGGCTGCAAAATTAAA
  TCTAGACATTCTTTTAATGCCACCACACGTGTTCCGCTTCTCTCTTTTAAAGTATTTATA
  AAAATATAAATTGTACATTTTGTAAAATATTATGTTTGATTTCTCTACTTGTCATATCAC
  TAAATAAACACGATTTTATTGCAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002514
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002514.2, NP_002505.1
• RefSeq Size: 2389 bp
• RefSeq ORF: 1074 bp
• Locus ID: 4856
• Cytogenetics: 8q24.12
• Domains: IB, tsp_1, VWC, CT, Cys_knot
• Protein Families: ES Cell Differentiation/IPS, Secreted Protein
• Gene Summary: 'The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development. [provided by RefSeq, Feb 2009]'