Karyopherin beta 3 (IPO5) (NM_002271) Human Untagged Clone

CAT#: SC118726

IPO5 (untagged)-Human importin 5 (IPO5)


  "NM_002271" in other vectors (7)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol IPO5
Synonyms IMB3; imp5; KPNB3; Pse1; RANBP5
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_002271, the custom clone sequence may differ by one or more nucleotides


ATGCCTGAGGATCAAGTTGGAAAACTAGAAGCAACAGAAAACACAATAAGCGCAATGGCGGCGGCCGCGG
CGGAGCAGCAACAGTTCTACCTGCTCCTGGGAAACCTGCTCAGCCCCGACAATGTGGTCCGGAAACAGGC
AGAGGAAACCTATGAGAATATCCCAGGCCAGTCAAAGATCACATTCCTCTTACAAGCCATCAGAAATACA
ACAGCTGCTGAAGAGGCTAGACAAATGGCCGCCGTTCTCCTAAGACGTCTCTTGTCCTCTGCATTTGATG
AAGTCTATCCAGCACTTCCCTCTGATGTTCAGACTGCCATCAAGAGTGAGCTACTCATGATTATTCAGAT
GGAAACACAATCTAGCATGAGGAAAAAAGTTTGTGATATTGCGGCAGAACTGGCCAGGAATTTAATAGAT
GAGGATGGCAATAACCAGTGGCCCGAAGGTTTGAAGTTCCTTTTTGATTCAGTCAGCTCTCAAAATGTGG
GACTGCGGGAAGCTGCCCTTCACATTTTCTGGAACTTTCCTGGAATTTTTGGGAACCAGCAACAACACTA
TTTAGATGTCATCAAACGAATGTTAGTTCAGTGTATGCAAGATCAGGAACACCCGTCGATCAGGACGTTA
TCTGCTAGAGCTACAGCTGCATTTATACTTGCAAATGAGCATAATGTTGCTCTGTTCAAACATTTTGCAG
ACTTGCTACCGGGATTCCTACAGGCGGTAAATGACTCGTGCTACCAGAATGATGATTCTGTCCTAAAATC
CCTCGTTGAGATTGCAGATACTGTTCCAAAGTATTTGCGTCCTCACTTGGAAGCAACTCTACAGCTAAGT
CTAAAGTTGTGTGGAGACACTAGCCTCAACAATATGCAACGCCAGCTTGCCCTTGAAGTGATCGTCACCC
TCTCTGAGACTGCAGCTGCTATGTTAAGAAAACATACCAATATTGTTGCACAGACTATTCCTCAGATGTT
AGCAATGATGGTTGATTTGGAAGAAGATGAGGACTGGGCAAATGCAGATGAACTAGAAGATGATGATTTT
GACAGCAATGCAGTTGCAGGCGAGAGTGCTCTAGATCGAATGGCTTGCGGACTTGGTGGAAAGCTCGTTC
TGCCGATGATCAAGGAACACATTATGCAAATGCTTCAAAATCCTGACTGGAAATACCGGCATGCAGGATT
GATGGCCTTATCTGCCATTGGTGAAGGGTGCCACCAGCAAATGGAAGGAATTCTAAATGAGATCGTAAAT
TTTGTTTTACTTTTTCTCCAGGATCCTCATCCAAGAGTAAGGTATGCAGCCTGTAATGCCGTGGGACAGA
TGGCTACAGATTTTGCACCTGGTTTCCAAAAGAAATTTCATGAGAAGGTGATTGCAGCTCTGCTGCAGAC
CATGGAAGACCAAGGCAATCAACGTGTGCAGGCCCATGCAGCTGCTGCCCTCATTAACTTTACTGAAGAC
TGTCCCAAGTCACTACTTATTCCATACTTGGATAATTTGGTGAAACATCTGCATTCCATTATGGTACTGA
AGCTTCAAGAGCTGATTCAGAAAGGCACCAAGTTAGTTTTGGAACAAGTTGTGACATCCATTGCATCAGT
TGCCGATACTGCAGAAGAAAAATTTGTCCCCTACTATGATTTATTTATGCCATCACTGAAGCACATCGTT
GAGAATGCGGTTCAAAAAGAACTGAGACTTCTGAGAGGAAAAACTATTGAATGCATTAGCCTCATTGGTC
TGGCTGTTGGGAAGGAAAAATTCATGCAGGATGCATCAGATGTGATGCAGCTTTTGTTAAAGACCCAGAC
AGACTTCAATGATATGGAAGATGATGATCCTCAGATCTCTTACATGATCTCAGCATGGGCCAGAATGTGC
AAAATCCTTGGAAAAGAATTTCAGCAATACCTTCCAGTGGTTATGGGGCCTTTAATGAAGACGGCTTCAA
TTAAGCCCGAAGTAGCCCTTTTAGATACCCAAGACATGGAGAATATGAGTGATGATGATGGTTGGGAATT
TGTGAACCTTGGAGATCAGCAAAGCTTTGGTATTAAAACTGCAGGACTAGAAGAAAAATCAACTGCTTGC
CAGATGTTGGTTTGCTATGCTAAGGAGTTAAAGGAAGGCTTTGTGGAGTACACCGAACAGGTTGTCAAAC
TGATGGTCCCTTTACTGAAATTTTATTTCCACGATGGTGTTCGAGTGGCAGCAGCGGAATCCATGCCTCT
TCTCCTGGAGTGTGCAAGAGTCCGTGGTCCTGAGTATCTCACACAGATGTGGCATTTTATGTGTGATGCT
CTAATTAAGGCCATTGGTACAGAACCAGATTCAGACGTCCTCTCAGAAATAATGCATTCTTTTGCAAAGT
GCATTGAAGTAATGGGAGATGGATGCCTTAATAATGAACACTTTGAAGAACTGGGAGGTATATTGAAAGC
AAAGCTTGAAGAACATTTTAAAAATCAAGAATTACGACAAGTTAAAAGACAAGATGAAGACTATGATGAA
CAGGTCGAAGAGTCACTACAAGATGAGGATGATAATGATGTTTATATTCTGACCAAAGTGTCAGATATTT
TACACTCAATATTCAGTAGCTACAAAGAAAAGGTGTTACCATGGTTTGAACAGCTGCTTCCATTAATTGT
CAACCTCATTTGTCCACATAGACCATGGCCAGACAGACAATGGGGATTATGCATCTTTGATGATGTCATA
GAACACTGTAGTCCAGCCTCATTTAAATACGCAGAATATTTCTTAAGACCAATGCTCCAATATGTATGTG
ACAACAGCCCAGAAGTCAGGCAAGCAGCTGCATATGGCCTGGGAGTCATGGCACAGTACGGTGGAGATAA
TTATCGCCCTTTTTGTACAGAAGCACTTCCCCTGCTGGTAAGAGTTATTCAGTCTGCGGATTCTAAGACC
AAAGAAAATGTCAATGCTACAGAGAACTGCATCTCAGCAGTAGGGAAAATCATGAAGTTCAAGCCTGACT
GTGTAAACGTTGAAGAGGTCCTTCCACACTGGTTGTCTTGGCTTCCACTACATGAAGATAAAGAAGAAGC
TGTTCAGACTTTCAATTATCTGTGTGACCTGATTGAAAGTAATCATCCAATTGTTCTTGGCCCAAACAAT
ACCAATCTGCCCAAAATATTTAGTATAATTGCGGAAGGAGAAATGCACGAGGCAATTAAACATGAAGATC
CTTGTGCCAAACGTCTGGCCAATGTCGTTCGCCAAGTACAGACTTCTGGAGGACTGTGGACTGAGTGCAT
AGCACAGCTCAGTCCTGAGCAGCAGGCCGCCATTCAGGAGCTCCTGAACTCTGCGTGA


>OriGene 5' read for NM_002271 unedited
CGGCCGCGAATTCGGCACCAGCGGCCGCGGCGGAGTGAGAGGCCGCGAGGCCCCGCCCCG
TCCTCCCCTTTCCCCTTTGCCCCGCCCTTCCCGCGCGGCCCCCCGCAAGCCCCGCGCCGC
CGCTGGTGCCGGTCCCCGCGCTGGGCCCGCCCCCGCCCCTCCCGCGGCCCGCGAGCGCGC
CTCACGGCTCCTGTCTCCCCTCCCTCCTTCTCTCTCACGCCTAGCGCAATGGCGGCGGCC
GCGGCGGAGCAGCAACAGTTCTACCTGCTCCTGGGAAACCTGCTCAGCCCCGACAATGTG
GTCCGGAAACAGGCAGAGGAAACCTATGAGAATATCCCAGGCCAGTCAAAGATCACATTC
CTCTTACAAGCCATCAGAAATACAACAGCTGCTGAAGAGGCTAGACAAATGGCCGCCGTT
CTCCTAAGACGTCTCTTGTCCTCTGCATTTGATGAAGTCTATCCAGCACTTCCCTCTGAT
GTTCAGACTGCCATCAAGAGTGAGCTACTCATGATTATTCAGATGGAAACACAATCTAGC
ATGAGGAAAAAAGTTTGTGATATTGCGGCAGAACTGGCCAGGAATTTAATAGATGAGGAT
GGCAATAACCAGTGGCCCGAAGGTTTGAAGTTCCTTTTTGATTCAGTCAGCTCTCAAAAT
GTGGGACTGCGGGAAGCTGCCCTTCACATTNTCTGGAACTTTCCTGGNATT
>OriGene 3' read for NM_002271 unedited
CCCCTTACTGTGCCACCGCGGCCGCATTTCACGACCGGGTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTAAGAAAAACCCTTCTATTTAAAATTCTGATCTATGCATAAAA
TTCATTTTTATATTCCGGTTAAATTTAGCCCAAACTATAAAAAGGTTACCCCTGAAGTTT
TCACCAGAAAGTCTATTAGGCAGGCCTTACAAACCTCTCCCCCCCCGCGGTCTTTTCCTG
CTATGAGGTTAATCCCTACCGAAACATTCCCCCTTTTTACTGAAATTTTCAGTAACCAAC
TTTACCCATACCCTTTATAACTAACCCCCCACACCGTCGGGGGCGGTCCTACCCCCATTC
CCCTTCCCCACGCACCCCCTTACCCCCAACCTTTCTGCCCCGCCCCCACCAACCTCCCCT
CCCACACTGCCTGCCCTTCCCTCCCCTCTCCCCCACCCCCCCCTCCCCACGGCCACGGTA
TCCTCATTCTCCCTTCACCCGCCCCCACGGCCCCCCCCCCCTCCCTCACCCCTCCCCCCC
CCCCCGCCACCCCCCCCCCCCCAACCCCTCCCCCCCCCGGGCCTCCCCTCCTTCCCCCCC
ACATCCCTCTCCCCTCCCCCCCCCCCCCCCACCCCCCCCCACTCCTACCCGCCCCACAAC
TCCCCCCCCCCCAACCCCCCCCCACCCCCCTCTTCTCATTCCTTTCCCCCAACTATCCCC
CTCATACCCTTATCCTCACCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCCCCCCCC
CCCCCTCCCCCATCCCAGCCCCCATCCACCCCCCCCCCCCCTCCCCCCTCCCCCCCCCCC
CTCCTCCTCCCCCCCCCCCCCCTCCTCTCCCCCTCCCCATTCACAACCCCACACTCCCTA
CCCCGTCTTTCTCCTCCCCCCCCCCTCATCT
Restriction Sites NotI-NotI     
ACCN NM_002271
Insert Size 4470 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002271.2, NP_002262.2
RefSeq Size 6019 bp
RefSeq ORF 3294 bp
Locus ID 3843
Cytogenetics 13q32.2
Protein Families Druggable Genome
Gene Summary 'Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. [provided by RefSeq, Jul 2008]'

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