Karyopherin beta 3 (IPO5) (NM_002271) Human Untagged Clone

CAT#: SC118726

IPO5 (untagged)-Human importin 5 (IPO5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IPO5
• Synonyms: IMB3; imp5; KPNB3; Pse1; RANBP5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002271, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCTGAGGATCAAGTTGGAAAACTAGAAGCAACAGAAAACACAATAAGCGCAATGGCGGCGGCCGCGG
  CGGAGCAGCAACAGTTCTACCTGCTCCTGGGAAACCTGCTCAGCCCCGACAATGTGGTCCGGAAACAGGC
  AGAGGAAACCTATGAGAATATCCCAGGCCAGTCAAAGATCACATTCCTCTTACAAGCCATCAGAAATACA
  ACAGCTGCTGAAGAGGCTAGACAAATGGCCGCCGTTCTCCTAAGACGTCTCTTGTCCTCTGCATTTGATG
  AAGTCTATCCAGCACTTCCCTCTGATGTTCAGACTGCCATCAAGAGTGAGCTACTCATGATTATTCAGAT
  GGAAACACAATCTAGCATGAGGAAAAAAGTTTGTGATATTGCGGCAGAACTGGCCAGGAATTTAATAGAT
  GAGGATGGCAATAACCAGTGGCCCGAAGGTTTGAAGTTCCTTTTTGATTCAGTCAGCTCTCAAAATGTGG
  GACTGCGGGAAGCTGCCCTTCACATTTTCTGGAACTTTCCTGGAATTTTTGGGAACCAGCAACAACACTA
  TTTAGATGTCATCAAACGAATGTTAGTTCAGTGTATGCAAGATCAGGAACACCCGTCGATCAGGACGTTA
  TCTGCTAGAGCTACAGCTGCATTTATACTTGCAAATGAGCATAATGTTGCTCTGTTCAAACATTTTGCAG
  ACTTGCTACCGGGATTCCTACAGGCGGTAAATGACTCGTGCTACCAGAATGATGATTCTGTCCTAAAATC
  CCTCGTTGAGATTGCAGATACTGTTCCAAAGTATTTGCGTCCTCACTTGGAAGCAACTCTACAGCTAAGT
  CTAAAGTTGTGTGGAGACACTAGCCTCAACAATATGCAACGCCAGCTTGCCCTTGAAGTGATCGTCACCC
  TCTCTGAGACTGCAGCTGCTATGTTAAGAAAACATACCAATATTGTTGCACAGACTATTCCTCAGATGTT
  AGCAATGATGGTTGATTTGGAAGAAGATGAGGACTGGGCAAATGCAGATGAACTAGAAGATGATGATTTT
  GACAGCAATGCAGTTGCAGGCGAGAGTGCTCTAGATCGAATGGCTTGCGGACTTGGTGGAAAGCTCGTTC
  TGCCGATGATCAAGGAACACATTATGCAAATGCTTCAAAATCCTGACTGGAAATACCGGCATGCAGGATT
  GATGGCCTTATCTGCCATTGGTGAAGGGTGCCACCAGCAAATGGAAGGAATTCTAAATGAGATCGTAAAT
  TTTGTTTTACTTTTTCTCCAGGATCCTCATCCAAGAGTAAGGTATGCAGCCTGTAATGCCGTGGGACAGA
  TGGCTACAGATTTTGCACCTGGTTTCCAAAAGAAATTTCATGAGAAGGTGATTGCAGCTCTGCTGCAGAC
  CATGGAAGACCAAGGCAATCAACGTGTGCAGGCCCATGCAGCTGCTGCCCTCATTAACTTTACTGAAGAC
  TGTCCCAAGTCACTACTTATTCCATACTTGGATAATTTGGTGAAACATCTGCATTCCATTATGGTACTGA
  AGCTTCAAGAGCTGATTCAGAAAGGCACCAAGTTAGTTTTGGAACAAGTTGTGACATCCATTGCATCAGT
  TGCCGATACTGCAGAAGAAAAATTTGTCCCCTACTATGATTTATTTATGCCATCACTGAAGCACATCGTT
  GAGAATGCGGTTCAAAAAGAACTGAGACTTCTGAGAGGAAAAACTATTGAATGCATTAGCCTCATTGGTC
  TGGCTGTTGGGAAGGAAAAATTCATGCAGGATGCATCAGATGTGATGCAGCTTTTGTTAAAGACCCAGAC
  AGACTTCAATGATATGGAAGATGATGATCCTCAGATCTCTTACATGATCTCAGCATGGGCCAGAATGTGC
  AAAATCCTTGGAAAAGAATTTCAGCAATACCTTCCAGTGGTTATGGGGCCTTTAATGAAGACGGCTTCAA
  TTAAGCCCGAAGTAGCCCTTTTAGATACCCAAGACATGGAGAATATGAGTGATGATGATGGTTGGGAATT
  TGTGAACCTTGGAGATCAGCAAAGCTTTGGTATTAAAACTGCAGGACTAGAAGAAAAATCAACTGCTTGC
  CAGATGTTGGTTTGCTATGCTAAGGAGTTAAAGGAAGGCTTTGTGGAGTACACCGAACAGGTTGTCAAAC
  TGATGGTCCCTTTACTGAAATTTTATTTCCACGATGGTGTTCGAGTGGCAGCAGCGGAATCCATGCCTCT
  TCTCCTGGAGTGTGCAAGAGTCCGTGGTCCTGAGTATCTCACACAGATGTGGCATTTTATGTGTGATGCT
  CTAATTAAGGCCATTGGTACAGAACCAGATTCAGACGTCCTCTCAGAAATAATGCATTCTTTTGCAAAGT
  GCATTGAAGTAATGGGAGATGGATGCCTTAATAATGAACACTTTGAAGAACTGGGAGGTATATTGAAAGC
  AAAGCTTGAAGAACATTTTAAAAATCAAGAATTACGACAAGTTAAAAGACAAGATGAAGACTATGATGAA
  CAGGTCGAAGAGTCACTACAAGATGAGGATGATAATGATGTTTATATTCTGACCAAAGTGTCAGATATTT
  TACACTCAATATTCAGTAGCTACAAAGAAAAGGTGTTACCATGGTTTGAACAGCTGCTTCCATTAATTGT
  CAACCTCATTTGTCCACATAGACCATGGCCAGACAGACAATGGGGATTATGCATCTTTGATGATGTCATA
  GAACACTGTAGTCCAGCCTCATTTAAATACGCAGAATATTTCTTAAGACCAATGCTCCAATATGTATGTG
  ACAACAGCCCAGAAGTCAGGCAAGCAGCTGCATATGGCCTGGGAGTCATGGCACAGTACGGTGGAGATAA
  TTATCGCCCTTTTTGTACAGAAGCACTTCCCCTGCTGGTAAGAGTTATTCAGTCTGCGGATTCTAAGACC
  AAAGAAAATGTCAATGCTACAGAGAACTGCATCTCAGCAGTAGGGAAAATCATGAAGTTCAAGCCTGACT
  GTGTAAACGTTGAAGAGGTCCTTCCACACTGGTTGTCTTGGCTTCCACTACATGAAGATAAAGAAGAAGC
  TGTTCAGACTTTCAATTATCTGTGTGACCTGATTGAAAGTAATCATCCAATTGTTCTTGGCCCAAACAAT
  ACCAATCTGCCCAAAATATTTAGTATAATTGCGGAAGGAGAAATGCACGAGGCAATTAAACATGAAGATC
  CTTGTGCCAAACGTCTGGCCAATGTCGTTCGCCAAGTACAGACTTCTGGAGGACTGTGGACTGAGTGCAT
  AGCACAGCTCAGTCCTGAGCAGCAGGCCGCCATTCAGGAGCTCCTGAACTCTGCGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002271 unedited
  CGGCCGCGAATTCGGCACCAGCGGCCGCGGCGGAGTGAGAGGCCGCGAGGCCCCGCCCCG
  TCCTCCCCTTTCCCCTTTGCCCCGCCCTTCCCGCGCGGCCCCCCGCAAGCCCCGCGCCGC
  CGCTGGTGCCGGTCCCCGCGCTGGGCCCGCCCCCGCCCCTCCCGCGGCCCGCGAGCGCGC
  CTCACGGCTCCTGTCTCCCCTCCCTCCTTCTCTCTCACGCCTAGCGCAATGGCGGCGGCC
  GCGGCGGAGCAGCAACAGTTCTACCTGCTCCTGGGAAACCTGCTCAGCCCCGACAATGTG
  GTCCGGAAACAGGCAGAGGAAACCTATGAGAATATCCCAGGCCAGTCAAAGATCACATTC
  CTCTTACAAGCCATCAGAAATACAACAGCTGCTGAAGAGGCTAGACAAATGGCCGCCGTT
  CTCCTAAGACGTCTCTTGTCCTCTGCATTTGATGAAGTCTATCCAGCACTTCCCTCTGAT
  GTTCAGACTGCCATCAAGAGTGAGCTACTCATGATTATTCAGATGGAAACACAATCTAGC
  ATGAGGAAAAAAGTTTGTGATATTGCGGCAGAACTGGCCAGGAATTTAATAGATGAGGAT
  GGCAATAACCAGTGGCCCGAAGGTTTGAAGTTCCTTTTTGATTCAGTCAGCTCTCAAAAT
  GTGGGACTGCGGGAAGCTGCCCTTCACATTNTCTGGAACTTTCCTGGNATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002271 unedited
  CCCCTTACTGTGCCACCGCGGCCGCATTTCACGACCGGGTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTAAGAAAAACCCTTCTATTTAAAATTCTGATCTATGCATAAAA
  TTCATTTTTATATTCCGGTTAAATTTAGCCCAAACTATAAAAAGGTTACCCCTGAAGTTT
  TCACCAGAAAGTCTATTAGGCAGGCCTTACAAACCTCTCCCCCCCCGCGGTCTTTTCCTG
  CTATGAGGTTAATCCCTACCGAAACATTCCCCCTTTTTACTGAAATTTTCAGTAACCAAC
  TTTACCCATACCCTTTATAACTAACCCCCCACACCGTCGGGGGCGGTCCTACCCCCATTC
  CCCTTCCCCACGCACCCCCTTACCCCCAACCTTTCTGCCCCGCCCCCACCAACCTCCCCT
  CCCACACTGCCTGCCCTTCCCTCCCCTCTCCCCCACCCCCCCCTCCCCACGGCCACGGTA
  TCCTCATTCTCCCTTCACCCGCCCCCACGGCCCCCCCCCCCTCCCTCACCCCTCCCCCCC
  CCCCCGCCACCCCCCCCCCCCCAACCCCTCCCCCCCCCGGGCCTCCCCTCCTTCCCCCCC
  ACATCCCTCTCCCCTCCCCCCCCCCCCCCCACCCCCCCCCACTCCTACCCGCCCCACAAC
  TCCCCCCCCCCCAACCCCCCCCCACCCCCCTCTTCTCATTCCTTTCCCCCAACTATCCCC
  CTCATACCCTTATCCTCACCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCCCCCCCCC
  CCCCCTCCCCCATCCCAGCCCCCATCCACCCCCCCCCCCCCTCCCCCCTCCCCCCCCCCC
  CTCCTCCTCCCCCCCCCCCCCCTCCTCTCCCCCTCCCCATTCACAACCCCACACTCCCTA
  CCCCGTCTTTCTCCTCCCCCCCCCCTCATCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002271
• Insert Size: 4470 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002271.2, NP_002262.2
• RefSeq Size: 6019 bp
• RefSeq ORF: 3294 bp
• Locus ID: 3843
• Cytogenetics: 13q32.2
• Protein Families: Druggable Genome
• Gene Summary: 'Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. [provided by RefSeq, Jul 2008]'