BLK (NM_001715) Human Untagged Clone

CAT#: SC119056

BLK (untagged)-Human B lymphoid tyrosine kinase (BLK)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BLK
• Synonyms: MODY11
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001715 edited
  GTTGGAAGTTGCTCGTTGTCGCTAGGAGCCTGCTCCACTGTAAGGGTGTCGGGATCTGAA
  GAGCTATGGTGAAACACCACTGAAGCATTGCCAAGGATGGGGCTGGTAAGTAGCAAAAAG
  CCGGACAAGGAAAAGCCGATCAAAGAGAAGGACAAGGGCCAATGGAGCCCCCTGAAGGTC
  AGCGCCCAAGACAAGGACGCCCCGCCACTGCCGCCCCTGGTTGTCTTCAACCACCTTACT
  CCTCCACCGCCCGATGAACACCTGGATGAAGACAAGCATTTCGTGGTGGCTCTGTATGAC
  TACACCGCTATGAATGATCGGGACCTGCAGATGCTGAAGGGGGAGAAGCTACAGGTCCTG
  AAGGGAACTGGAGACTGGTGGCTGGCCAGGTCACTCGTCACAGGAAGAGAAGGCTATGTG
  CCCAGTAACTTTGTGGCCCGAGTGGAGAGCCTGGAAATGGAAAGGTGGTTCTTTAGATCA
  CAGGGTCGGAAGGAGGCTGAGAGGCAGCTTCTTGCTCCAATCAACAAGGCCGGCTCCTTT
  CTTATCAGAGAGAGTGAAACCAACAAAGGTGCCTTCTCCCTGTCTGTGAAGGATGTCACC
  ACCCAGGGGGAGCTGATCAAGCACTATAAGATCCGCTGCCTGGATGAAGGGGGCTACTAC
  ATCTCCCCCCGGATCACCTTCCCCTCGCTCCAGGCCCTGGTGCAGCACTATTCTAAGAAG
  GGGGATGGTCTATGCCAGAGGCTGACCCTGCCCTGTGTGCGCCCGGCCCCGCAGAATCCC
  TGGGCCCAGGATGAATGGGAGATCCCCCGGCAGTCTCTCAGGCTGGTCAGGAAACTCGGG
  TCTGGACAATTCGGCGAAGTCTGGATGGGTTACTACAAAAACAACATGAAGGTGGCCATT
  AAGACGCTGAAGGAGGGAACCATGTCTCCAGAAGCCTTCCTGGGTGAGGCCAACGTGATG
  AAGGCTCTGCAGCACGAGCGGCTGGTCCGACTCTACGCAGTGGTCACCAAGGAGCCCATC
  TACATTGTCACCGAGTACATGGCCAGAGGATGCCTGCTGGATTTCCTGAAGACAGATGAA
  GGGAGCAGATTGTCACTCCCAAGGCTGATTGACATGTCGGCGCAGATTGCTGAAGGGATG
  GCATACATTGAGCGCATGAATTCCATCCACCGCGACCTGCGGGCGGCCAACATCCTGGTG
  TCTGAGGCCTTGTGCTGCAAAATTGCTGATTTTGGCTTGGCTCGAATCATCGACAGTGAA
  TACACGGCCCAAGAGGGGGCCAAGTTCCCCATCAAGTGGACAGCCCCGGAAGCCATCCAC
  TTCGGGGTCTTCACCATCAAAGCAGACGTGTGGTCGTTTGGAGTCCTCCTGATGGAAGTT
  GTCACTTATGGGCGGGTGCCATACCCAGGGATGAGCAACCCCGAGGTCATCCGCAACCTG
  GAGCGCGGCTACCGCATGCCGCGCCCCGACACCTGCCCGCCCGAGCTGTACCGCGGCGTC
  ATCGCCGAGTGCTGGCGCAGCCGGCCCGAGGAGCGGCCCACCTTCGAGTTCCTGCAGTCG
  GTGCTGGAGGACTTCTACACGGCCACCGAGCGGCAGTACGAGCTGCAGCCCTAGCCGGCC
  GCGCCCGCCTGCGCCCCGTGCCCACCTCTGCGCGGACGACCCCGACTTCCGTGCCATCCC
  AGACGGGCCGCGAAGGCGGGGTGTCGCCTGTGCCCTTTTCTCAGACCCGGAATCCAGTGG
  GCAGAGGCAGCTTCGCAGGGGGTCCCCGGACGGACTCCTTCACCGACTGTACCCCCGGGC
  GAGTTACGCGGCCTCTCTGTGCCGCTTCATTTGTAGAGGGCTGTAACAGTGACCTCGCAC
  GGTCATCCGGAGTACTAAGCCCCAGTAAGGTGTTCAGGACTGGTAAGCGACTGTCATCAA
  GTAAGGCCCCCGTGCTGGGCACCCCCCGTGCTGGCCGCGTCCCCGCCTCTGCGCCCTGCG
  TGGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001715 unedited
  CAGGATTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGGAGGCTCT
  GATCGCAGACCGGGGGTGCTGCTACCTCTGTCTGCTGCCGGCAGAAAGCCACAAGCCATG
  AAAACTGATTGAGATGAGAAGAATTCATCTGGGACTGGCTTTTGCTTTAGGATGGTGTTG
  GAAGTTGCTCGTTGTCGCTAGGAGCCTGCTCCACTGTAAGGGTGTCGGGATCTGAAGAGC
  TATGGTGAAACACCACTGAAGCATTGCCAAGGATGGGGCTGGTAAGTAGCAAAAAGCCGG
  ACAAGGAAAAGCCGATCAAAGAGAAGGACAAGGGCCAATGGAGCCCCCTGAAGGTCAGCG
  CCCAAGACAAGGACGCCCCGCCACTGCCGCCCCTGGTTGTCTTCAACCACCTTACTCCTC
  CACCGCCCGATGAACACCTGGATGAAGACAAGCATTTCGTGGTGGCTCTGTATGACTACA
  CCGCTATGAATGATCGGGACCTGCAGATGCTGAAGGGGGAGAAGCTACAGGTCCTGAAGG
  GAACTGGAGACTGGTGGCTGGCCAGGTCACTCGTCACAGGAAGAGAAGGCTATGTGCCCA
  GTAACTTTGTGGCCCGAGTGGAGAGCCTGGAAATGGAAAGGTGGTTCTTTAGATCACAGG
  GTCGGAAGGAGGCTGAGAGGCAGCTTCTTGCTCCAATCAACAAGGCCGGCTCCTTTCTTA
  TCAGAGAGAGTGAANACCAACAAGGTGCCTTCTCCCTGTCTGTGAAGGATGTCACCACCC
  CNGGGGGAGCTGATCAAGCACTATNAGATCCGCTGCCTGGATGAAGGGGGCTACTACATC
  TCCCCCCGGATCACCTTNCCTCGCTNNCAGCCCTGGTGCAGCACTATTTCTAGAAGGGGG
  ATGGTCTATGCCAGAGCTGACCTGCCCTGTGTGCGCCCGGCCCGCAAATCCCTGGCCCAG
  ATGAATGGGGATCCCCGGCAGTCTTTCAGCTGGTAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001715 unedited
  NNAAAATACTTGNACCGCGCCGCATTCTAGNGACTAGTCTCGATTTTTTTTTTTTTTTTT
  TTTTTTTCGAACGCTCGTGACTTTATTGCAAAAAAGCCCCGCAGTGCTGGAGCGCAGCCT
  AGGCTGGGGCTGCCCCTGCTGGCGTCCGCGGGACCCAGTCTGGCTTCTGTAGCGGGGCAG
  GGCGGGGTCCACGCAGGGCGCAAAGGCGGGGACGCGGCCAGCACGGGGGGTGCCCAGCAC
  GGGGGCCTTACTTGATGACAGTCGCTTACCAGTCCTGAACACCTTACTGGGGCTTAGTAC
  TCCGGATGACCGTGCGAGGTCACTGTTACAGCCCTCTACAAATGAAGCGGCACAGAGAGG
  CCGCGTAACTCGCCCGGGGGTACAGTCGGTGAAGGAGTCCGTCCGGGGACCCCCTGCGAA
  GCTGCCTCTGCCCACTGGATTCCGGGTCTGAGAAAAGGGCACAGGCGACACCCCGCCTTC
  GCGGCCCGTCTGGGATGGCACGGAAGTCGGGGTCGTCCGCGCAAAGGTGGGCACGGGGCG
  CAGGCGGGCGCGGCCGGCTAGGGCTGCAGCTCGTACTGCCGCTCGGTGGCCGTGTAGAAG
  TCCTCCAGCACCGACTGCAGGAACTCGAAGGTGGGCCGCTCCTCGGGCCGGCTGCGCCAG
  CACTCGGCGATGACGCCGCGGTACAGCTCGGGCGGGCAGGNTGTCGGGGCGCGGCATGCG
  GTAGCCGCGCTCCAGGTTGCGGATGACCTCGGGGTTGCTCATCCCTGGGTATGGCACCCG
  CCCATAAGTGACAACTTCCATCAGGAGGACTCCAACGACCACACGTCTGCTTTGATGGTG
  AAGACCCCCGAGTGGATGGCTTCCGGGGCTGTCCACTTGATGGGGAAACTTGGCCCCCTC
  TGGGGCCGTGTATTCACTGTCGATGATTCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001715
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001715.2, NP_001706.2
• RefSeq Size: 2642 bp
• RefSeq ORF: 1518 bp
• Locus ID: 640
• Cytogenetics: 8p23.1
• Domains: pkinase, SH2, TyrKc, SH3, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: 'This gene encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors. [provided by RefSeq, Aug 2010]'