BLK (NM_001715) Human Untagged Clone

CAT#: SC323504

BLK (untagged)-Kinase deficient mutant (K269M) of Human B lymphoid tyrosine kinase (BLK)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BLK
• Synonyms: MODY11
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323504 sequence for NM_001715 edited (data generated by NextGen Sequencing)
  ATGGGGCTGGTAAGTAGCAAAAAGCCGGACAAGGAAAAGCCGATCAAAGAGAAGGACAAG
  GGCCAATGGAGCCCCCTGAAGGTCAGCGCCCAAGACAAGGACGCCCCGCCACTGCCGCCC
  CTGGTTGTCTTCAACCACCTTACTCCTCCACCGCCCGATGAACACCTGGATGAAGACAAG
  CATTTCGTGGTGGCTCTGTATGACTACACCGCTATGAATGATCGGGACCTGCAGATGCTG
  AAGGGGGAGAAGCTACAGGTCCTGAAGGGAACTGGAGACTGGTGGCTGGCCAGGTCACTC
  GTCACAGGAAGAGAAGGCTATGTGCCCAGTAACTTTGTGGCCCGAGTGGAGAGCCTGGAA
  ATGGAAAGGTGGTTCTTTAGATCACAGGGTCGGAAGGAGGCTGAGAGGCAGCTTCTTGCT
  CCAATCAACAAGGCCGGCTCCTTTCTTATCAGAGAGAGTGAAACCAACAAAGGTGCCTTC
  TCCCTGTCTGTGAAGGATGTCACCACCCAGGGGGAGCTGATCAAGCACTATAAGATCCGC
  TGCCTGGATGAAGGGGGCTACTACATCTCCCCCCGGATCACCTTCCCCTCGCTCCAGGCC
  CTGGTGCAGCACTATTCTAAGAAGGGGGATGGTCTATGCCAGAGGCTGACCCTGCCCTGT
  GTGCGCCCGGCCCCGCAGAATCCCTGGGCCCAGGATGAATGGGAGATCCCCCGGCAGTCT
  CTCAGGCTGGTCAGGAAACTCGGGTCTGGACAATTCGGCGAAGTCTGGATGGGTTACCAC
  AAAAACAACATGAAGGTGGCCATTATGACGCTGAAGGAGGGAACCATGTCTCCAGAAGCC
  TTCCTGGGTGAGGCCAACGTGATGAAGGCTCTGCAGCACGAGCGGCTGGTCCGACTCTAC
  GCAGTGGTCACCAAGGAGCCCATCTACATTGTCACCGAGTACATGGCCAGAGGATGCCTG
  CTGGATTTCCTGAAGACAGATGAAGGGAGCAGATTGTCACTCCCAAGGCTGATTGACATG
  TCGGCGCAGATTGCTGAAGGGATGGCATACATTGAGCGCATGAATTCCATCCACCGCGAC
  CTGCGGGCGGCCAACATCCTGGTGTCTGAGGCCTTGTGCTGCAAAATTGCTGATTTTGGC
  TTGGCTCGAATCATCGACAGTGAATACACGGCCCAAGAGGGGGCCAAGTTCCCCATCAAG
  TGGACAGCCCCGGAAGCCATCCACTTCGGGGTCTTCACCATCAAAGCAGACGTGTGGTCG
  TTTGGAGTCCTCCTGATGGAAGTTGTCACTTATGGGCGGGTGCCATACCCAGGGATGAGC
  AACCCCGAGGTCATCCGCAACCTGGAGCGCGGCTACCGCATGCCGCGCCCCGACACCTGC
  CCGCCCGAGCTGTACCGCGGCGTCATCGCCGAGTGCTGGCGCAGCCGGCCCGAGGAGCGG
  CCCACCTTCGAGTTCCTGCAGTCGGTGCTGGAGGACTTCTACACGGCCACCGAGCGGCAG
  TACGAGCTGCAGCCCTAG
  
  Clone variation with respect to NM_001715.2
  778 t=>c;806 a=>t;843 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_001715 unedited
  ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAGGCTCTGATCGC
  AGACCGGGGGTGCTGCTACCTCTGTCTGCTGCCGGCAGAAAGCCACAAGCCATGAAAACTGATTGAGATG
  AGAAGAATTCATCTGGGACTGGCTTTTGCTTTAGGATGGTGTTGGAAGTTGCTCGTTGTCGCTAGGAGCC
  TGCTCCACTGTAAGGGTGTCGGGATCTGAAGAGCTATGGTGAAACACCACTGAAGCATTGCCAAGGATGG
  GGGCTGGGTAAGTAGCAAAAAGCCGGACAAGGAAAAGCCGATCAAAGAGAAGGACAAGGGCCAATGGAGC
  CCCCCTGAGGTCAGCCGCCCCAAGACCAAGGACGCCCCCGCCACTGCCCGCCCCCTGTTTGTTCTTCAAC
  CCACCCTACCTCCTTCCACCCGCCCGATGAACCACCCTGAATGAAGAACAAGCATTTTCGTGGTGGGCCT
  CGTGTATGGACTCACCCCGCTATGAATTGATCCGGAACCTGGCAAATGCTGAAGGGGGGAAACCTACAGG
  TCCTGAAGGAAACTGGAGACGTGGGGGTGGGCCCAGTTACACTCGTCACAGAGAAAGAAGGCTATGTGCC
  GTTAACTTTGGGCCAGTGTGAAACCCTGAAATGAAAGAGGGTCTCTTATATCAAGGTTCAAAGAGCTAAA
  GGATCTTGGCTAACACAGGCGCTCTTTTTACAGAAGTTGAACCCAAGTGCCTACTCGTTGGAAGTTCCAC
  CCAAGGAACTTAGCCCATAATTCCGTCGTAAGGTCTAATCCGGATCCCTCTCCCCTCCAAGCGTGCCATT
  AGCACAAGAGATGTTC
  
  Kinase domain sequence

  >SC323504 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TCTGGTAGACTCGGGTCTGGACATTCGGCGAAGTCTGGATGGGTTACCACAAAAACAACATGAAGGTGGC
  CATTATGACGCTGAAGGAGGGAACCATGTCTCCAGAAGCCTTCCTGGGTGAGGCCAACGTGATGAAGGCT
  CTGCAGCACGAGCGGCTGGTCCGACTCTACGCAGTGGTCACCAAGGAGCCCATCTACATTGTCACCGAGT
  ACATGGCCAGAGGATGCCTGCTGGATTTCCTGAAGACAGATGAAG
  
• Restriction Sites: Please inquire
• ACCN: NM_001715
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001715.2, NP_001706.2
• RefSeq Size: 2642 bp
• RefSeq ORF: 1518 bp
• Locus ID: 640
• Cytogenetics: 8p23.1
• Domains: pkinase, SH2, TyrKc, SH3, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: 'This gene encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors. [provided by RefSeq, Aug 2010]'