AARE (APEH) (NM_001640) Human Untagged Clone

CAT#: SC119119

APEH (untagged)-Human N-acylaminoacyl-peptide hydrolase (APEH)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: APEH
• Synonyms: AARE; ACPH; APH; D3F15S2; D3S48E; DNF15S2; OPH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001640, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAACGTCAGGTGCTGCTGAGCGAGCCCGAGGAGGCGGCGGCTCTGTATCGGGGCCTTAGCCGCCAGC
  CCGCGCTGAGCGCCGCCTGCCTGGGCCCGGAGGTCACCACGCAGTACGGCGGCCAATACCGGACGGTGCA
  CACTGAGTGGACCCAGAGGGACCTGGAACGCATGGAGAACATTCGATTCTGCCGCCAATACCTGGTGTTC
  CATGACGGGGACTCAGTGGTGTTTGCAGGACCTGCAGGCAACAGTGTGGAGACCCGGGGGGAACTGCTGA
  GCAGAGAGTCTCCTTCAGGCACCATGAAAGCTGTGCTGCGCAAGGCTGGAGGCACGGGCCCTGGGGAAGA
  GAAGCAGTTCCTGGAGGTCTGGGAGAAGAACCGGAAGCTCAAGAGCTTCAACCTGTCAGCGCTGGAGAAA
  CATGGGCCTGTTTATGAGGATGACTGCTTTGGCTGCCTGTCCTGGTCGCACTCGGAGACACACTTGTTGT
  ATGTGGCAGAGAAGAAGCGCCCCAAGGCCGAGTCCTTCTTTCAGACCAAAGCCTTGGACGTCAGTGCCAG
  CGATGATGAGATAGCCAGGCTGAAGAAGCCAGACCAAGCCATCAAGGGGGATCAGTTTGTGTTTTATGAA
  GACTGGGGAGAAAACATGGTTTCCAAAAGCATCCCTGTGCTCTGCGTGCTGGATGTCGAGAGTGGCAACA
  TCTCTGTGCTTGAGGGGGTCCCTGAGAATGTGTCCCCTGGACAGGCATTTTGGGCCCCTGGAGATGCTGG
  TGTGGTGTTTGTGGGCTGGTGGCATGAGCCCTTCCGGTTGGGCATCCGCTTTTGCACCAATCGCAGGTCA
  GCCCTGTATTATGTGGACCTCATCGGGGGGAAGTGTGAGCTCCTCTCGGATGACTCCCTGGCTGTCTCTT
  CTCCCCGGCTGAGCCCAGACCAATGTCGCATTGTCTACCTGCAGTACCCATCTCTGATCCCCCATCACCA
  ATGCAGCCAGCTGTGCCTGTATGACTGGTATACCAAGGTTACCTCAGTGGTGGTAGATGTTGTGCCTCGG
  CAGCTGGGAGAGAACTTCTCTGGGATCTACTGCAGCCTTCTGCCTTTGGGATGCTGGTCAGCTGACAGCC
  AGAGAGTGGTCTTTGACTCGGCTCAGCGCAGCCGGCAGGACCTGTTTGCTGTGGACACCCAAGTGGGCAC
  TGTGACCTCCCTCACAGCTGGAGGGTCAGGTGGGAGCTGGAAGTTGCTCACAATTGACCAGGACCTCATG
  GTGGCACAGTTTTCCACACCCAGCCTACCTCCAACCCTGAAAGTTGGGTTCCTGCCTTCTGCAGGGAAGG
  AGCAGTCAGTGTTGTGGGTGTCCCTGGAGGAGGCCGAGCCCATTCCCGACATCCACTGGGGCATCCGGGT
  GCTACAGCCACCCCCAGAGCAAGAGAATGTGCAGTATGCTGGCCTTGACTTTGAAGCAATCCTGCTGCAG
  CCTGGCAGCCCTCCAGATAAGACCCAAGTGCCCATGGTGGTCATGCCCCACGGGGGGCCCCATTCATCCT
  TTGTCACTGCCTGGATGCTGTTCCCAGCCATGCTTTGCAAGATGGGCTTTGCGGTACTACTAGTGAACTA
  TCGTGGCTCCACGGGCTTTGGCCAGGACAGCATCCTCTCCCTCCCAGGCAATGTGGGCCACCAGGATGTG
  AAGGATGTCCAGTTTGCAGTGGAACAGGTGCTCCAGGAGGAACACTTTGATGCAAGCCATGTGGCCCTTA
  TGGGTGGTTCCCATGGTGGCTTCATTTCCTGCCACTTGATTGGTCAGTACCCAGAGACCTACAGGGCCTG
  CGTGGCCCGGAACCCCGTGATCAACATCGCCTCCATGTTGGGCTCCACTGACATCCCTGACTGGTGCGTG
  GTGGAGGCTGGCTTTCCTTTCAGCAGTGACTGCCTGCCAGACCTCAGCGTGTGGGCTGAGATGCTGGACA
  AATCGCCCATCAGATACATCCCTCAGGTGAAGACACCACTGTTACTGATGTTGGGCCAGGAGGACCGGCG
  TGTGCCCTTCAAGCAGGGCATGGAGTATTACCGTGCCCTCAAGACCCGGAATGTGCCTGTTCGGCTCCTG
  CTCTATCCCAAAAGCACCCACGCATTATCAGAGGTGGAGGTGGAGTCAGACAGCTTCATGAATGCTGTGC
  TCTGGCTACGCACACACTTGGGCAGCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001640 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAGACTATGGAAC
  GTCAGGTGCTGCTGAGCGAGCCCGAGGAGGCGGCGGCTCTGTATCGGGGCCTTAGCCGCC
  AGCCCGCGCTGAGCGCCGCCTGCCTGGGCCCGGAGGTCACCACGCAGTACGGCGGCCAAT
  ACCGGACGGTGCACACTGACTGCTGAGCAGAGAGTCTCCTTCAGGCACCATGAAAGCTGT
  GCTGCGCAAGGCTGGAGGCACGGGCCCTGGGGAAGAGAAGCAGTTCCTGGAGGTCTGGGA
  GAAGAACCGGAAGCTCAAGAGCTTCAACCTGTCAGCGCTGGAGAAACATGGGCCTGTTTA
  TGAGGATGACTGCTTTGGCTGCCTGTCCTGGTCGCACTCGGAGACACACTTGTTGTATGT
  GGCAGAGAAGAAGCGCCCCAAGGCCGAGTCCTTCTTTCAGACCAAAGCCTTGGACGTCAG
  TGCCAGCGATGATGAGATAGCCAGGCTGAAGAAGCCAGACCAAGCCATAAGGGGGGATCA
  GGTTTTGTGGTTTATGAAAGACTGGNGGAGAAAACATTGGTTTCCAAAAGCATCCCTGTG
  TTCTGCGTGTTTGGAATGTCAAGAGTGGCACATCTCTTGCTGGAGGGGGGGGTCCTGAAA
  AATGTGTCCCCTGACAGGCATTTTGGCCCTGAATCTGGGGNGGGGTTTTTGGGGGGGGTG
  GGGCATAACCTCTTCCGGGTGGCAATCCGTTTGACAAATCGCAGTAGCCTGGTTTATGGG
  ACTATCGGGGGGGGAAAGGGGGACCCTTTGGAGGATCCTGGGCTGGCTTTTTCCCCGTTG
  ACCAAACATGGTCCATTGGTACTGAATACCATTTTTGACCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001640
• Insert Size: 2920 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001640.3, NP_001631.3
• RefSeq Size: 2775 bp
• RefSeq ORF: 2199 bp
• Locus ID: 327
• Cytogenetics: 3p21.31
• Domains: Peptidase_S9
• Protein Families: Druggable Genome, Protease
• Gene Summary: ' This gene encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma. [provided by RefSeq, Jul 2008]'