AARE (APEH) (NM_001640) Human Untagged Clone

CAT#: SC324594

APEH (untagged)-Human N-acylaminoacyl-peptide hydrolase (APEH)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: APEH
• Synonyms: AARE; ACPH; APH; D3F15S2; D3S48E; DNF15S2; OPH
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001640.3 CTCAACCCCTTCTCTCGTAGGCCCTTCTACCCTCCCCGAAGCCGGGCTCCCGGGTTCCGC
  TTCCACAGGGCCGCCCCCTCTCCTCCGAGATCCAACGCCTCGCCCCTCGGTAAGAGGCCC
  AGGACTCAGCCCACGGCCCCGCCCCCCACTTGGCTAGTCCGCCCCTGGCGAGACCCCTGC
  TCTCACCCACCCAGGACTTCTCGCTCCCAGGCCGGGTCCTCTCACCGTCAAGGCCCGCCC
  CCTGCCAACGGTCCTCACCCATTAGCCGAAGGCCCCGCCCCTCACAGATTGCCGCAGGGC
  AGGGGCGGAGACAGCCCGGGCCGTCCCAGGCTCCGCCCCCGGAAGCCTCACTTCCGGGCG
  CGAGCACGCCCCGCCTCGCCCCGGCGGCAGAGAGGAGACTATGGAACGTCAGGTGCTGCT
  GAGCGAGCCCGAGGAGGCGGCGGCTCTGTATCGGGGCCTTAGCCGCCAGCCCGCGCTGAG
  CGCCGCCTGCCTGGGCCCGGAGGTCACCACGCAGTACGGCGGCCAATACCGGACGGTGCA
  CACTGAGTGGACCCAGAGGGACCTGGAACGCATGGAGAACATTCGATTCTGCCGCCAATA
  CCTGGTGTTCCATGACGGGGACTCAGTGGTGTTTGCAGGACCTGCAGGCAACAGTGTGGA
  GACCCGGGGGGAACTGCTGAGCAGAGAGTCTCCTTCAGGCACCATGAAAGCTGTGCTGCG
  CAAGGCTGGAGGCACGGGCCCTGGGGAAGAGAAGCAGTTCCTGGAGGTCTGGGAGAAGAA
  CCGGAAGCTCAAGAGCTTCAACCTGTCAGCGCTGGAGAAACATGGGCCTGTTTATGAGGA
  TGACTGCTTTGGCTGCCTGTCCTGGTCGCACTCGGAGACACACTTGTTGTATGTGGCAGA
  GAAGAAGCGCCCCAAGGCCGAGTCCTTCTTTCAGACCAAAGCCTTGGACGTCAGTGCCAG
  CGATGATGAGATAGCCAGGCTGAAGAAGCCAGACCAAGCCATCAAGGGGGATCAGTTTGT
  GTTTTATGAAGACTGGGGAGAAAACATGGTTTCCAAAAGCATCCCTGTGCTCTGCGTGCT
  GGATGTCGAGAGTGGCAACATCTCTGTGCTTGAGGGGGTCCCTGAGAATGTGTCCCCTGG
  ACAGGCATTTTGGGCCCCTGGAGATGCTGGTGTGGTGTTTGTGGGCTGGTGGCATGAGCC
  CTTCCGGTTGGGCATCCGCTTTTGCACCAATCGCAGGTCAGCCCTGTATTACGTGGACCT
  CATCGGGGGGAAGTGTGAGCTCCTCTCGGATGACTCCCTGGCTGTCTCTTCTCCCCGGCT
  GAGCCCAGACCAATGTCGCATTGTCTACCTGCAGTACCCATCTCTGATCCCCCATCACCA
  ATGCAGCCAGCTGTGCCTGTATGACTGGTATACCAAGGTTACCTCAGTGGTGGTAGATGT
  TGTGCCTCGGCAGCTGGGAGAGAACTTCTCTGGGATCTACTGCAGCCTTCTGCCTTTGGG
  ATGCTGGTCAGCTGACAGCCAGAGAGTGGTCTTTGACTCGGCTCAGCGCAGCCGGCAGGA
  CCTGTTTGCTGTGGACACCCAAGTGGGCACTGTGACCTCCCTCACAGCTGGAGGGTCAGG
  TGGGAGCTGGAAGTTGCTCACAATTGACCAGGACCTCATGGTGGCACAGTTTTCCACACC
  CAGCCTACCTCCAACCCTGAAAGTTGGGTTCCTGCCTTCTGCAGGGAAGGAGCAGTCAGT
  GTTGTGGGTGTCCCTGGAGGAGGCCGAGCCCATTCCCGACATCCACTGGGGCATCCGGGT
  GCTACAGCCACCCCCAGAGCAAGAGAATGTGCAGTATGCTGGCCTTGACTTTGAAGCAAT
  CCTGCTGCAGCCTGGCAGCCCTCCAGATAAGACCCAAGTGCCCATGGTGGTCATGCCCCA
  CGGGGGGCCCCATTCATCCTTTGTCACTGCCTGGATGCTGTTCCCAGCCATGCTTTGCAA
  GATGGGCTTTGCGGTACTACTAGTGAACTATCGTGGCTCCACGGGCTTTGGCCAGGACAG
  CATCCTCTCCCTCCCAGGCAATGTGGGCCACCAGGATGTGAAGGATGTCCAGTTTGCAGT
  GGAACAGGTGCTCCAGGAGGAACACTTTGATGCAAGCCATGTGGCCCTTATGGGTGGTTC
  CCATGGTGGCTTCATTTCCTGCCACTTGATTGGTCAGTACCCAGAGACCTACAGGGCCTG
  CGTGGCCCGGAACCCCGTGATCAACATCGCCTCCATGTTGGGCTCCACTGACATCCCTGA
  CTGGTGCGTGGTGGAGGCTGGCTTTCCTTTCAGCAGTGACTGCCTGCCAGACCTCAGCGT
  GTGGGCTGAGATGCTGGACAAATCGCCCATCAGATACATCCCTCAGGTGAAGACACCACT
  GTTACTGATGTTGGGCCAGGAGGACCGGCGTGTGCCCTTCAAGCAGGGCATGGAGTATTA
  CCGTGCCCTCAAGACCCGGAATGTGCCTGTTCGGCTCCTGCTCTATCCCAAAAGCACCCA
  CGCATTATCAGAGGTGGAGGTGGAGTCAGACAGCTTCATGAATGCTGTGCTCTGGCTACG
  CACACACTTGGGCAGCTGAAGCCCTGCCATTCTGCATGAGCTGATCAGCCTGTGCCACAC
  TTCGCTCTTGAGGAGCTCAACGGTCTGGCAGGGCAGCAGGAGGCTTTCTGGGCTCTGGAC
  TCCACGGATGCGTGGGCAGAGGAATGTGGGCTATGTAGTCATAATAAATTAGGACACAAA
  AAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001640
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001640.3, NP_001631.3
• RefSeq Size: 2775 bp
• RefSeq ORF: 2199 bp
• Locus ID: 327
• Cytogenetics: 3p21.31
• Domains: Peptidase_S9
• Protein Families: Druggable Genome, Protease
• Gene Summary: ' This gene encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma. [provided by RefSeq, Jul 2008]'