Islet 1 (ISL1) (NM_002202) Human Untagged Clone

CAT#: SC125257

ISL1 (untagged)-Human ISL LIM homeobox 1 (ISL1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ISL1
• Synonyms: Isl-1; ISLET1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125257 sequence for NM_002202 edited (data generated by NextGen Sequencing)
  ATGGGAGACATGGGAGATCCACCAAAAAAAAAACGTCTGATTTCCCTATGTGTTGGTTGC
  GGCAATCAGATTCACGATCAGTATATTCTGAGGGTTTCTCCGGATTTGGAATGGCATGCG
  GCATGTTTGAAATGTGCGGAGTGTAATCAGTATTTGGACGAGAGCTGTACATGCTTTGTT
  AGGGATGGGAAAACCTACTGTAAAAGAGATTATATCAGGTTGTACGGGATCAAATGCGCC
  AAGTGCAGCATCGGCTTCAGCAAGAACGACTTCGTGATGCGTGCCCGCTCCAAGGTGTAT
  CACATCGAGTGTTTCCGCTGTGTGGCCTGCAGCCGCCAGCTCATCCCTGGGGACGAATTT
  GCGCTTCGGGAGGACGGTCTCTTCTGCCGAGCAGACCACGATGTGGTGGAGAGGGCCAGT
  CTAGGCGCTGGCGACCCGCTCAGTCCCCTGCATCCAGCGCGGCCACTGCAAATGGCAGCG
  GAGCCCATCTCCGCCAGGCAGCCAGCCCTGCGGCCCCACGTCCACAAGCAGCCGGAGAAG
  ACCACCCGCGTGCGGACTGTGCTGAACGAGAAGCAGCTGCACACCTTGCGGACCTGCTAC
  GCCGCAAACCCGCGGCCAGATGCGCTCATGAAGGAGCAACTGGTAGAGATGACGGGCCTC
  AGTCCCCGTGTGATCCGGGTCTGGTTTCAAAACAAGCGGTGCAAGGACAAGAAGCGAAGC
  ATCATGATGAAGCAACTCCAGCAGCAGCAGCCCAATGACAAAACTAATATCCAGGGGATG
  ACAGGAACTCCCATGGTGGCTGCCAGTCCAGAGAGACACGACGGTGGCTTACAGGCTAAC
  CCAGTGGAAGTACAAAGTTACCAGCCACCTTGGAAAGTACTGAGCGACTTCGCCTTGCAG
  AGTGACATAGATCAGCCTGCTTTTCAGCAACTGGTCAATTTTTCAGAAGGAGGACCGGGC
  TCTAATTCCACTGGCAGTGAAGTAGCATCAATGTCCTCTCAACTTCCAGATACACCTAAC
  AGCATGGTAGCCAGTCCTATTGAGGCATGA
  
  Clone variation with respect to NM_002202.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002202 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCGGCGCGGCCGGGAAGG
  AACGCGGGAGGGGACAGAAGGAAGAGGAAGAGGAGGAGAGGGAGGCCAGAGCCAGAACAG
  CCCGGCAGCCCGAGCTTCGGGGGAGAACGGCCTGAGCCCCGAGCAAGTTGCCTCGGGAGC
  CCTAATCCTCTCCCGCTGGCTCGCCGAGCGGTCAGTGGCGCTCAGCGGCGGCGAGGCTGA
  AATATGATAATCAGAACAGCTGCGCCGCGCGCCCTGCAGCCAATGGGCGCGGCGCTCGCC
  TGACGTCCCCGCGCGCTGCGTCAGACCAATGGCGATGGAGCTGAGTTGGAGCAGAGAAGT
  TTGAGTAAGAGATAAGGAAGAGAGGTGCCCGAGCCGCGCCGAGTCTGCCGCCGCCGCAGC
  GCCTCCGCTCCGCCAACTCCGCCGGCTTAAATTGGACTCCTAGATCCGCGAGGGCGCGGC
  GCAGCCGAGCAGCGGCTCTTTCAGCATTGGCAACCCCAGGGGCCCAATATTTCCCAACTT
  AGCCACAGCTCCAGCANTCCTCTCTGTGGGCTGTTCACCAACTGTACAACCACCATTTCA
  CTGTGGACATTACTCCCTCTTACAGATATGGGAGACATGGGAGATCCACCAAAAAAAAAA
  CGTCTGATTTCCCTATGTGTTGGTGCGGCAATCAGATTCACGATCAGTATATTCTGAGGG
  TTTCTCCGGATTGGAATGGCATGCGGCATGTTTGAAATGTGCGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002202 unedited
  AACTTCCCGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTT
  CAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTT
  TCCTTTTTATTTCCATAGAGAGAGAAAACAGTCATTTTAACCAATAACTATACACATCTT
  TGGGGGAAAAGTTCTTGGACAGACACAAGTCAAACACAATCCCGAGACGCTTGTGGCAAA
  ATAGAGGTAACCTTGTTGCAATGCTTTATAAGTTGGTTTTTAAATCTGAATTCAAAAACC
  TTTATAGTCGCTTCAGACTTCTTGATAAAGGATGGATCTCAAAAAAAGTGGGGAGGGGGC
  AAAGTAAAAAATAAACCAGCTACAGGACAGGCCAAGAGACCCAGGATTTCAATTAACCCC
  TTGTTAAAGTCAACTTTAAACATATCAAACCAATGCAGCTCCACCTTCCTGAAAGGACTC
  TTTCAGCCAAGGAGGCCAGTCTCTCTTCCTGCGCTTTTGCTGGAGCTCCTGTTTGGCAAG
  GCAATGACCAATATAGAAACAGCACCATTCAGATTAGGATGGACTAGAAAATTTTCTATT
  TTTGTTTTTCTCTAGATGTGCAGTGAAATGAAAATGCTTTCTTTGAGATCCTTGAATATA
  AATCCTCTCGTTTTAAAAACGTCTTTTTTTTTAGTACTTCTAGATCATTAATAGCATATA
  CTGGTTTTGCGTTTTGNTGTGGTAAATCTAGTAAATC
  
• Restriction Sites: Please inquire
• ACCN: NM_002202
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002202.1, NP_002193.1
• RefSeq Size: 2397 bp
• RefSeq ORF: 1041 bp
• Locus ID: 3670
• Cytogenetics: 5q11.1
• Protein Families: ES Cell Differentiation/IPS, Transcription Factors
• Gene Summary: 'This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq, Jul 2008]'