Islet 1 (ISL1) (NM_002202) Human Untagged Clone

CAT#: SC322127

ISL1 (untagged)-Human ISL LIM homeobox 1 (ISL1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ISL1
• Synonyms: Isl-1; ISLET1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322127 TGAAGGAAGAGGAAGAGGAGGAGAGGGAGGCCAGAGCCAGAACAGCCCGGCAGCCCGGGC
  TTCGGGGGAGAACGGCCTGAGCCCCGAGCAAGTTGCCTCGGGAGCCCTAATCCTCTCCCG
  CTGGCTCGCCGAGCGGTCAGTGGCGCTCAGCGGCGGCGAGGCTGAAATATGATAATCAGA
  ACAGCTGCGCCGCGCGCCCTGCAGCCAATGGGCGCGGCGCTCGCCTGACGTCCCCGCGCG
  CTGCGTCAGACCAATGGCGATGGAGCTGAGTTGGAGCAGAGAAGTTTGAGTAAGAGATAA
  GGAAGAGAGGTGCCCGAGCCGCGCCGAGTCTGCCGCCGCCGCAGCGCCTCCGCTCCGCCA
  ACTCCGCCGGCTTAAATTGGAATCCTAGATCCGCGAGGGCGCGGCGCAGCCGAGCAGCGG
  CTCTTTCAGCATTGGCAACCCCAGGGGCCAATATTTCCCACTTAGCCACAGCTCCAGCAT
  CCTCTCTGTGGGCTGTTCACCAGCTGTACAACCACCATTTCACTGTGGACATTACTCCCT
  CTTACAGATATGGGAGACATGGGAGATCCACCAAAAAAAAAACGTCTGATTTCCCTATGT
  GTTGGTTGCGGCAATCAGATTCACGATCAGTATATTCTGAGGGTTTCTCCGGATTTGGAA
  TGGCATGCGGCATGTTTGAAATGTGCGGAGTGTAATCAGTATTTGGACGAGAGCTGTACA
  TGCTTTGTTAGGGATGGGAAAACCTACTGTAAAAGAGATTATATCAGGTTGTACGGGATC
  AAATGCGCCAAGTGCAGCATCGGCTTCAGCAAGAACGACTTCGTGATGCGTGCCCGCTCC
  AAGGTGTATCACATCGAGTGTTTCCGCTGTGTGGCCTGCAGCCGCCAGCTCATCCCTGGG
  GACGAATTTGCGCTTCGGGAGGACGGTCTCTTCTGCCGAGCAGACCACGATGTGGTGGAG
  AGGGCCAGTCTAGGCGCTGGCGACCCGCTCAGTCCCCTGCATCCAGCGCGGCCACTGCAA
  ATGGCAGCGGAGCCCATCTCCGCCAGGCAGCCGGCCCTGCGGCCCCACGTCCACAAGCAG
  CCGGAGAAGACCACCCGCGTGCGGACTGTGCTGAACGAGAAGCAGCTGCACACCTTGCGG
  ACCTGCTACGCCGCAAACCCGCGGCCAGATGCGCTCATGAAGGAGCAACTGGTAGAGATG
  ACGGGCCTCAGTCCCCGTGTGATCCGGGTCTGGTTTCAAAACAAGCGGTGCAAGGACAAG
  AAGCGAAGCATCATGATGAAGCAACTCCAGCAGCAGCAGCCCAATGACAAAACTAATATC
  CAGGGGATGACAGGAACTCCCATGGTGGCTGCCAGTCCAGAGAGACACGACGGTGGCTTA
  CAGGCTAACCCAGTGGAAGTACAAAGTTACCAGCCACCTTGGAAAGTACTGAGCGACTTC
  GCCTTGCAGAGTGACATAGATCAGCCTGCTTTTCAGCAACTGGTCAATTTTTCAGAAGGA
  GGACCGGGCTCTAATTCCACTGGCAGTGAAGTAGCATCAATGTCCTCTCAACTTCCAGAT
  ACACCTAACAGCATGGTAGCCAGTCCTATTGAGGCATGAGGAACATTCATTCTGTATTTT
  TTTTCCCTGTTGGAGAAAGTGGGAAATTATAATGTCGAACTCTGAAACAAAAGTATTTAA
  CGACCCAGTCAATGAAAACTGAATCAAGAAATGAATGCTCCATGAAATGCACGAAGTCTG
  TTTTAATGACAAGGTGATATGGTAGCAACACTGTGAAGACAATCATGGGATTTTACTAGA
  ATTAAACAACAAACAAAACGCAAAACCCAGTATATGCTATTCAATGATCTTAGAAGTACT
  GAAAAAAAAAGACGTTTTTAAAACGTAGAGGATTTATATTCAAGGATCTCAAAGAAAGCA
  TTTTCATTTCACTGCACATCTAGAGAAAAACAAAAATAGAAAATTTTCTAGTCCATCCTA
  ATCTGAATGGTGCTGTTTCTATATTGGTCATTGCCTTGCCAAACAGGAGCTCCAGCAAAA
  GCGCAGGAAGAGAGACTGGCCTCCTTGGCTGAAAGAGTCCTTTCAGGAAGGTGGAGCTGC
  ATTGGTTTGATATGTTTAAAGTTGACTTTAACAAGGGGTTAATTGAAATCCTGGGTCTCT
  TGGCCTGTCCTGTAGCTGGTTTATTTTTTACTTTGCCCCCTCCCCACTTTTTTTGAGATC
  CATCCTTTATCAAGAAGTCTGAAGCGACTTTAAAGGTTTTTGAATTCAGATTTAAAAACC
  AACTTATAAAGCATTGCAACAAGGTTACCTCTATTTTGCCACAAGCGTCTCGGGATTGTG
  TTTGACTTGTGTCTGTCCAAGAACTTTTCCCCCAAAGATGTGTATAGTTATTGGTTAAAA
  TGACTGTTTTCTCTCTCTATGGAAATAAAAAGGAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002202
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002202.2, NP_002193.2
• RefSeq Size: 2729 bp
• RefSeq ORF: 1050 bp
• Locus ID: 3670
• Cytogenetics: 5q11.1
• Protein Families: ES Cell Differentiation/IPS, Transcription Factors
• Gene Summary: 'This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq, Jul 2008]'