Annexin VII (ANXA7) (NM_001156) Human Untagged Clone

CAT#: SC126802

ANXA7 (untagged)-Human annexin A7 (ANXA7), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ANXA7
• Synonyms: ANX7; SNX; SYNEXIN
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001156, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCATACCCAGGCTATCCCCCAACAGGCTACCCACCTTTCCCTGGATATCCTCCTGCAGGTCAGGAGT
  CATCTTTTCCCCCTTCTGGTCAGTATCCTTATCCTAGTGGCTTTCCTCCAATGGGAGGAGGTGCCTACCC
  ACAAGTGCCAAGTAGTGGCTACCCAGGAGCTGGAGGCTACCCTGCGCCTGGAGGTTATCCAGCCCCTGGA
  GGCTATCCTGGTGCCCCACAGCCAGGGGGAGCTCCATCCTATCCCGGAGTTCCTCCAGGCCAAGGATTTG
  GAGTCCCACCAGGTGGAGCAGGCTTTTCTGGGTATCCACAGCCACCTTCACAGTCTTATGGAGGTGGTCC
  AGCACAGGTTCCACTACCTGGTGGCTTTCCTGGAGGACAGATGCCTTCTCAGTATCCTGGAGGACAACCT
  ACTTACCCTAGTCAGCCTGCCACAGTGACTCAGGTCACTCAAGGAACTATCCGACCAGCTGCCAACTTCG
  ATGCTATAAGAGATGCAGAAATTCTTCGTAAGGCAATGAAGGGTTTTGGGACAGATGAGCAGGCAATTGT
  GGATGTGGTGGCCAACCGTTCCAATGATCAGAGGCAAAAAATTAAAGCAGCATTTAAGACCTCCTATGGC
  AAGGATTTAATCAAAGATCTCAAATCAGAGTTAAGTGGAAATATGGAAGAACTGATCCTGGCCCTCTTCA
  TGCCTCCTACGTATTACGATGCCTGGAGCTTACGGAAAGCAATGCAGGGAGCAGGAACTCAGGAACGTGT
  ATTGATTGAGATTTTGTGCACAAGAACAAATCAGGAAATCCGAGAAATTGTCAGATGTTATCAGTCAGAA
  TTTGGACGAGACCTTGAAAAGGACATTAGGTCAGATACATCAGGACATTTTGAACGTTTACTTGTGTCCA
  TGTGCCAGGGAAATCGTGATGAGAACCAGAGTATAAACCACCAAATGGCTCAGGAAGATGCTCAGCGTCT
  CTATCAAGCTGGTGAGGGGAGACTAGGGACCGATGAATCTTGCTTTAACATGATCCTTGCCACAAGAAGC
  TTTCCTCAGCTGAGAGCTACCATGGAGGCTTATTCTAGGATGGCTAATCGAGACTTGTTAAGCAGTGTGA
  GCCGTGAGTTTTCCGGATATGTAGAAAGTGGTTTGAAGACCATCTTGCAGTGTGCCCTGAACCGCCCTGC
  CTTCTTTGCTGAGAGGCTCTACTATGCTATGAAAGGTGCTGGCACAGATGACTCCACCCTGGTCCGGATT
  GTGGTCACTCGAAGTGAGATTGACCTTGTACAAATAAAACAGATGTTCGCTCAGATGTATCAGAAGACTC
  TGGGCACAATGATTGCAGGTGACACGAGTGGAGATTACCGAAGACTTCTTCTGGCTATTGTGGGCCAGTA
  G
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001156 unedited
  TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGTGACGCTGCTGCT
  GGGGTCAGAATGTCATACCCAGGCTATCCCCCAACAGGCTACCCACCTTTCCCTGGATAT
  CCTCCTGCAGGTCAGGAGTCATCTTTTCCCCCTTCTGGTCAGTATCCTTATCCTAGTGGC
  TTTCCTCCAATGGGAGGAGGTGCCTACCCACAAGTGCCAAGTAGTGGCTACCCAGGAGCT
  GGAGGCTACCCTGCGCCTGGAGGTTATCCAGCCCCTGGAGGCTATCCTGGTGCCCCACAG
  CCAGGGGGAGCTCCATCCTATCCCGGAGTTCCTCCAGGCCAAGGATTTGGAGTCCCACCA
  GGTGGAGCAGGCTTTTCTGGGTATCCACAGCCACCTTCACAGTCTTATGGAGGTGGTCCA
  GCACAGGTTCCACTACCTGGTGGCTTTCCTGGAGGACAGATGCCTTCTCAGTATCCTGGA
  GGACAACCTACTTACCCTAGTCAGATCAATACAGATTCTTTTTCTTCCTATCCTGTTTTC
  TCTCCTGTTTCTTTGGATTATAGCAGTGAACCTGCCACAGTGACTCAGGTCACTCAAGGA
  ACTATCCGACCAGCTGCCAACTTCGATGCTATAAGAGATGCAGAAATTCTTCGTAAGGCA
  ATGAAGGGTTTTGGGACAGATGAGCAGGCAATTGTGGATGTGGTGGCCAACCGTTCCCAT
  GATCAGAGGCANAAAATTAAAGCAGCATTTAAGACCTCCTATGGCAGGGATTTATCAAAG
  ATCTCAATCAGAGTTAAGTGGAATATGGAAGAACTGATCCCTGGCCTCTTCATGCCTNNC
  TACGTATACGATGCCTGNNAGCTACNGNAAGCATGCAGGNAGGCAGACTCANGACC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001156 unedited
  CAATATTGCAATTTTTTATTTGGTCTAACTCAATTTAAAAAAGAAAACACTGCGAAAAAA
  AGTAGGCTATGTATCTTAAGCATATAAACATTTGACCCTTTCTTAAAAGGTGCCACAGGT
  AATACTAATCTATTTTCTATGTGGAAAGTGTTTATGAATACAGAACCAATTTAGGCAAAA
  TGAGATGCAAATTATTCTAGATGTAAAGGTTGAATTTTTTTTTAAAGTACACAGTTTTAA
  CATACTATCCACTCTATCAAGCAGACTTAGAGGGATTCCGGAAGGCAGAGCTTTCAGTAA
  CCTTCTAAAAAATTATTCAATATTATTACACAGAATCAGAGATTGCTGTGATTTTTATTC
  AATTTGACATCTTGATTAGAATGAAACTAGTAAGAATGAAGGTTTACAAACATTGCAATA
  TTACTGTATCATTGTGATATGGCTTTACATTGATTGTATGTAGAGAACAAAATAAAATTA
  GAATTAAGGCAATAACAACATGTGCAAACCAAGCACATTACCCTGATACGGTCCTTGACA
  GAAAGCTCTTTCGGTTAGCTGATGTTTGATATTGCTGCATGCAGGTCATTGCTCTGAAGG
  ATAAGCTATGAATAGAAATTCTTTTTCATTAAAAAAAAAAAAATCCCTCCTACTGGCCCA
  CAATAGCCAGAAGAAGTCTTCGGTAATCTCCACTCGTGTCACCTGCAATCATTGTGCCCC
  AGAGTCTTCTGATACATCTGAGCGAACATCTGGTTTATTTGTACAAGTCAATCTCACTTC
  GAGTGACCACAATNCGGACCACGGTGGAGTCATCTGTGCCACCACCTTTCATAGCATAGT
  AGAGCCTCTCACCANAGAAGGCAGGGCGGGTCATGGCCCCTGCAAGATGGTCTCAACCAC
  CTTTTAATATCGGAAACCACGCTCAACTGNTTACAGGTTCGATAGCCTCCTAGATAAACC
  TCTTGTACTTCTACTTGAGAAGATTTTTGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001156
• Insert Size: 2320 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001156.2, NP_001147.1
• RefSeq Size: 2110 bp
• RefSeq ORF: 1401 bp
• Locus ID: 310
• Cytogenetics: 10q22.2
• Domains: annexin
• Gene Summary: 'Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3'-non coding regions by the use of two alternative poly(A) signals. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including voltage-sensitive calcium channel activity, ion selectivity and membrane fusion. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) lacks an alternate in-frame exon, compared to variant 2, resulting in a shorter protein (isoform 1) that lacks an internal segment, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data.