ATF 4 (ATF4) (NM_182810) Human Untagged Clone

CAT#: SC127457

ATF4 (untagged)-Human activating transcription factor 4 (tax-responsive enhancer element B67) (ATF4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATF4
• Synonyms: CREB-2; CREB2; TAXREB67; TXREB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127457 sequence for NM_182810 edited (data generated by NextGen Sequencing)
  ATGACCGAAATGAGCTTCCTGAGCAGCGAGGTGTTGGTGGGGGACTTGATGTCCCCCTTC
  GACCCGTCGGGTTTGGGGGCTGAAGAAAGCCTAGGTCTCTTAGATGATTACCTGGAGGTG
  GCCAAGCACTTCAAACCTCATGGGTTCTCCAGCGACAAGGCTAAGGCGGGCTCCTCCGAA
  TGGCTGGCTGTGGATGGGTTGGTCAGTCCCTCCAACAACAGCAAGGAGGATGCCTTCTCC
  GGGACAGATTGGATGTTGGAGAAAATGGATTTGAAGGAGTTCGACTTGGATGCCCTGTTG
  GGTATAGATGACCTGGAAACCATGCCAGATGACCTTCTGACCACGTTGGATGACACTTGT
  GATCTCTTTGCCCCCCTAGTCCAGGAGACTAATAAGCAGCCCCCCCAGACGGTGAACCCA
  ATTGGCCATCTCCCAGAAAGTTTAACAAAACCCGACCAGGTTGCCCCCTTCACCTTCTTA
  CAACCTCTTCCCCTTTCCCCAGGGGTCCTGTCCTCCACTCCAGATCATTCCTTTAGTTTA
  GAGCTGGGCAGTGAAGTGGATATCACTGAAGGAGATAGGAAGCCAGACTACACTGCTTAC
  GTTGCCATGATCCCTCAGTGCATAAAGGAGGAAGACACCCCTTCAGATAATGATAGTGGC
  ATCTGTATGAGCCCAGAGTCCTATCTGGGGTCTCCTCAGCACAGCCCCTCTACCAGGGGC
  TCTCCAAATAGGAGCCTCCCATCTCCAGGTGTTCTCTGTGGGTCTGCCCGTCCCAAACCT
  TACGATCCTCCTGGAGAGAAGATGGTAGCAGCAAAAGTAAAGGGTGAGAAACTGGATAAG
  AAGCTGAAAAAAATGGAGCAAAACAAGACAGCAGCCACTAGGTACCGCCAGAAGAAGAGG
  GCGGAGCAGGAGGCTCTTACTGGTGAGTGCAAAGAGCTGGAAAAGAAGAACGAGGCTCTA
  AAAGAGAGGGCGGATTCCCTGGCCAAGGAGATCCAGTACCTGAAAGATTTGATAGAAGAG
  GTCCGCAAGGCAAGGGGGAAGAAAAGGGTCCCCTAG
  
  Clone variation with respect to NM_182810.1
  65 a=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_182810 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGGTGGAAGAATCCCC
  TGGTCTCCGTGAGCGTCCATTTTGTGGAACCTGAGTTGCAAGCAGGGAGGGGCAAATACA
  ACTGCCCTGTTCCCGATTCTCTAGATGGCCGATCTAGAGAAGTCCCGCCTCATAAGTGGA
  AGGATGAAATTCTCAGAACAGCTAACCTCTAATGGGAGTTGGCTTCTGATTCTCATTCAG
  GCTTCTCACGGCATTCAGCAGCAGCGTTGCTGTAACCGACAAAGACACCTTCGAATTAAG
  CACATTCCTCGATTCCAGCAAAGCACCGCAACATGACCGAAATGAGCTTCCTGAGCAGCG
  AGGTGTTGGNTGGGGGACCTTGATGTCCCCCTTCGACCCGTCGGGTTTGGGGGCTGAAGA
  AAGCCTAGGTCTCTTAGATGATTACCTGGAGGTGGCCAAGCACTTCAAACCTCATGGGTT
  CTCCAGCGACAAGGCTAAGGCGGGCTCCTCCGAATGGCTGGCTGTGGATGGGTTGGGTCA
  GTCCCTCCAACAACAGCAAGGAGGATGCCTTCTCCGGGACAATTGGNATGTTGGAGAAAA
  TGGATTGAAAGAGTTCGACTTGGATGCCCTGTTGGGGTATAGATGACCTGGAAACCATGC
  CAGATGACCTTCTGACCACGTTGGATGACACTTGTGATCTCTTTGCCCCCCTAGTCCANG
  AGACTNAATAGCAGCCCCCCCAGACGGTGAACCCANTGGCCATCTCCCAGAAAGTTTACC
  ANACCCGACCAGGTTGCCCCCTTCACCTTCTACACCTCTTCCCTTCCCCAAGGGGGCCTG
  TCCTCCTCCAGACATNCCCTTANTTAAAACCGGCCGGGGAGGGGTATCCTGGAGAACAAG
  GA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_182810 unedited
  NNNNNNNNNNNNNNNTTTTAACTTTGAACCGCGGNCCGCATNCTANGATCGAGTTTTTTT
  TTTTTTTTTTTACTTTCCCTACAAAAAATTTATTGGGAACACACAGCTACAGCACTCTAT
  GTACAAGCACATTGACGCTCCTGACTATCCTCAACTAGGGGACCCTTTTCTTCCCCCTTG
  CCTTGCGGACCTCTTCTATCAAATCTTTCAGGTACTGGATCTCCTTGGCCAGGGAATCCG
  CCCTCTCTTTTAGAGCCTCGTTCTTCTTTTCCAGCTCTTTGCACTCACCAGTAAGAGCCT
  CCTGCTCCGCCCTCTTCTTCTGGCGGTACCTAGTGGCTGCTGTCTTGTTTTGCTCCATTT
  TTTTCAGCTTCTTATCCAGTTTCTCACCCTTTACTTTTGCTGCTACCATCTTCTCTCCAG
  GAGGATCGTAAGGTTTGGGACGGGCAGACCCACAGAGAACACCTGGAGATGGGAGGCTCC
  TATTTGGAGAGCCCCTGNTANAGGGGCTGTGCTGAGGAGACCCCANAAAGACTCTGGGCT
  CATACAGATGCCCTATCATTATCTGAAGGGGTGTCTTCCTCCTTTATGCACTGAGGGAAC
  ATGGCAACGTAAGCANTGTANTCCTGCCTTCCTATCTTCTTTAATGATATCCACTTAACT
  GGCCANCTTTAAACTAAAGGAATGATCTTGAGGGGAAGACAGGACCCCCGGGGAAAGGGG
  AAAAGTTTGAAAAATGGGAAGGGGGCACCCTGGTCGGTTTTGGAAAACTTTTGGGAAAAT
  GCCAATGGGGTTACCGCTGGGGGGGCCTCTTTTTAATCTCCGGGACTTAGGGGGCAAAAA
  ACACCAGTGTCCCCCCACGTGGCAAAAGCAATTGGCTGGGGTCCCGGCATTTTACCCCAG
  GGGGTTCCAAGGAAAACCCTTAAACCATTTTCCCAAACCAATTTGGCCCGAAAAGCTTCT
  CA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_182810
• Insert Size: 1410 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182810.1, NP_877962.1
• RefSeq Size: 1420 bp
• RefSeq ORF: 1056 bp
• Locus ID: 468
• Cytogenetics: 22q13.1
• Protein Families: Transcription Factors
• Protein Pathways: GnRH signaling pathway, Long-term potentiation, MAPK signaling pathway, Neurotrophin signaling pathway, Prostate cancer
• Gene Summary: 'This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011]'
  Transcript Variant: This variant (2) lacks an internal segment in the 5' UTR, compared to variant 1. The protein translation of this variant is regulated by two upstream open reading frames (PMID: 15277680). Both variants 1 and 2 encode the same protein.