SRP1 (KPNA1) (NM_002264) Human Untagged Clone

CAT#: SC128103

KPNA1 (untagged)-Human karyopherin alpha 1 (importin alpha 5) (KPNA1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KPNA1
• Synonyms: IPOA5; NPI-1; RCH2; SRP1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002264, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACCACCCCAGGAAAAGAGAACTTTCGCCTGAAAAGTTACAAGAACAAATCTCTGAATCCCGATGAGA
  TGCGCAGGAGGAGGGAGGAAGAAGGACTGCAGTTACGAAAGCAGAAAAGAGAAGAGCAGTTATTCAAGCG
  GAGAAATGTTGCTACAGCAGAAGAAGAAACAGAAGAAGAAGTTATGTCAGATGGAGGCTTTCATGAGGCT
  CAGATTAGTAACATGGAGATGGCACCAGGTGGTGTCATCACTTCTGACATGATTGAAATGATATTTTCCA
  AAAGCCCAGAGCAACAGCTTTCAGCAACACAGAAATTCAGGAAGCTGCTTTCAAAAGAACCTAACCCTCC
  TATTGATGAAGTTATCAGCACACCAGGAGTAGTGGCCAGGTTTGTGGAGTTCCTCAAACGAAAAGAGAAT
  TGTACACTGCAGTTTGAATCAGCTTGGGTACTGACAAATATTGCTTCAGGAAATTCTCTTCAGACCCGAA
  TTGTGATTCAGGCAGGAGCTGTGCCCATCTTCATAGAGTTGCTCAGCTCAGAGTTTGAAGATGTCCAGGA
  ACAGGCAGTCTGGGCTCTTGGCAACATTGCTGGAGATAGTACCATGTGCAGGGACTATGTCTTAGACTGC
  AATATCCTTCCCCCTCTTTTGCAGTTATTTTCAAAGCAAAACCGCCTGACCATGACCCGGAATGCAGTAT
  GGGCTTTGTCTAATCTCTGTAGAGGGAAAAGTCCACCTCCAGAATTTGCAAAGGTTTCTCCATGTCTGAA
  TGTGCTTTCCTGGTTGCTGTTTGTCAGTGACACTGATGTACTGGCTGATGCCTGCTGGGCCCTCTCATAT
  CTATCAGATGGACCCAATGATAAAATTCAAGCGGTCATCGATGCGGGAGTATGTAGGAGACTTGTGGAAC
  TGCTGATGCATAATGATTATAAAGTGGTTTCTCCTGCTTTGCGAGCTGTGGGAAACATTGTCACAGGGGA
  TGATATTCAGACACAGGTAATTCTGAATTGCTCAGCTCTGCAGAGTTTATTGCATTTGCTGAGTAGCCCA
  AAGGAATCTATCAAAAAGGAAGCATGTTGGACGATATCTAATATTACAGCTGGAAATAGGGCACAGATCC
  AGACTGTGATAGATGCCAACATTTTCCCAGCCCTCATTAGTATTTTACAAACTGCTGAATTTCGGACAAG
  AAAAGAAGCAGCTTGGGCCATCACAAATGCAACTTCTGGAGGATCAGCTGAACAGATCAAGTACCTAGTA
  GAACTGGGTTGTATCAAGCCGCTCTGTGATCTCCTCACGGTCATGGACTCTAAGATTGTACAGGTTGCCC
  TAAATGGCTTGGAAAATATCCTGAGGCTTGGAGAACAGGAAGCCAAAAGGAATGGCACTGGCATTAACCC
  TTACTGTGCTTTGATTGAAGAAGCTTATGGTCTGGATAAAATTGAGTTCTTACAGAGTCATGAAAACCAG
  GAGATCTACCAAAAGGCCTTTGATCTTATTGAGCATTACTTCGGGACCGAAGATGAAGACAGCAGCATTG
  CACCCCAGGTTGACCTTAACCAGCAGCAGTACATCTTCCAACAGTGTGAGGCTCCTATGGAAGGTTTCCA
  GCTTTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002264 unedited
  TGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCTGCTCGGTGCGAGGCGG
  CGGAGAGCGAGGCCTGGTGAGCACCGCCGAGGCGCGGGCCAGCTCTTCGAGGTTGTGCGC
  GGGAGTGGCACGGCGGGCGGGCGAGCGAGGGGCTAACTTCAGCGGTGGCACCGGGATCGG
  TTGCCTTGAGCCTGAAATCATGACCACCCCAGGAAAAGAGAACTTTCGCCTGAAAAGTTA
  CAAGAACAAATCTCTGAATCCCGATGAGATGCGCAGGAGGAGGGAGGAAGAAGGACTGCA
  GTTACGAAAGCAGAAAAGAGAAGAGCAGTTATTCAAGCGGAGAAATGTTGCTACAGCAGA
  AGAAGAAACAGAAGAAGAAGTTATGTCAGATGGAGGCTTTCATGAGGCTCAGATTAATAA
  CATGGAGATGGCACCAGGTGGTGTCATCACTTCTGACATGATTGAAATGATATTTTCCAA
  AAGCCCAGAGCAACAGCTTTCAGCAACACAGAAATTCAGGAAGCTGCTTTCAAAAGAACC
  TAACCCTCCTATTGATGAAGTTATCAGCACACCAGGAGTAGTGGCCAGGTTTGTGGAGTT
  CCTCAAACGAAAAGAGAATTGTACCACTGCAGTTTGAATCAGCTTTGGGTACTGACAAAT
  ATTGCTTCAGGAAATTCTCTTCAACCCGAATTGTGATTCAGCAGGAGCTGTGCCCATCTT
  CATAGAGTTGCTCAGCTCAGAGTTTGGAAGATGTCCAGGAACAGGGCAGTCTGGGCTCTT
  GGNCACATTGCTGNAGATAGTACCATGTGCAGGGACTATGTCTTAGACTGGCATATNCCT
  TCCCCTCTTTTGCAGTTATTTTCAAGCAAAACGCCTGACCTGACCCGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_002264 unedited
  NNNGGGCCCACTTGGGNGGTTGGCCACAATTCCCAGGGCCACAGNAATGAGCCACCTGGG
  GNAGTGGGCTCACAGGGNATGCCCACCCGGGATTCTGTTCAGGAAACAGCCACGACCGCC
  GGCCGCAATCTAGAGCCGACCCCCCCCCCCCCCCCCCCCCCCCCAGGAAAACCCCTTCAT
  AATGCATCCCCCAATCCGGGGGATTAAATCGCTGAACTGCCTACTAGCACTAGAGAACTA
  AACTCAAAGCCTGCCTGTGGGTAGACTTTACAATAGAACTGGTCTGACAAAGCTTGGTCT
  TATAAAATATACTTACATATTGTTTCTTAGGCAAAAATCGGCAGCAATCTGGAATAATTC
  AGATTTTTTATGAGATTAATTGCCAATCTTTTTATCTGTGACGATAATGCCAAAAATGAC
  TCAAAGCCAATAACCTTCATCTGTCATATGGAAAAAACAATCCTAGGGCAATATTTTTTT
  CAAAAGCGGAACTGACAAAGATAAGCGTTTAAAAGGGTCTTGATCAATTAGTTACAGGAA
  CATGAAAGAAAGTGTTCCACAGAGAACTGTGTCTCCTAAAATCTCTTTCTTCTATCATCT
  ACCTTACCCAAAATTTCTCAATCCAACCACATCTACCTGCTGGTCCATCAGATACCAGAT
  GCCTTTACATCCAAAATTCTCTTAATGCTTTTTTTCCCCCACCAAAGTTCTCATTACAAA
  TAATATAGATTTGNNAGTTTGGNAGGATGGAGGGGAATTAAAAAGGAAAAGTAAAACAAA
  GTCCCTTTTCCCCAATCCTATATAAAACTACAAAGATTTTTCCTGTATTTGAATTAACAT
  GCTTTTGTAATGAGCAACCCCTTATGGTAGGTTTTTCAGTGAATTTAAACTAAGTTAACA
  GAATCTTAAGGCCCAAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002264
• Insert Size: 4700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002264.2, NP_002255.2
• RefSeq Size: 6887 bp
• RefSeq ORF: 1617 bp
• Locus ID: 3836
• Cytogenetics: 3q21.1
• Domains: Armadillo_seg, IBB
• Gene Summary: 'The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. This protein interacts with the recombination activating gene 1 (RAG1) protein and is a putative substrate of the RAG1 ubiquitin ligase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]'
  Transcript Variant: This variant (1) lacks an alternate exon compared to variant 2 and represents the protein-coding transcript. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.