SRP1 (KPNA1) (NM_002264) Human Untagged Clone

CAT#: SC322070

KPNA1 (untagged)-Human karyopherin alpha 1 (importin alpha 5) (KPNA1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KPNA1
• Synonyms: IPOA5; NPI-1; RCH2; SRP1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322070 CGCTGCTCGGTGCGAGGCGGCGGAGAGCGAGGCCTGGTGAGCACCGCCGAGGCGCGGGCC
  AGCTCTTCGAGGTTGTGCGCGGGAGTGGCACGGCGGGCGGGCGAGCGAGGGGCTAACTTC
  AGCGGTGGCACCGGGATCGGTTGCCTTGAGCCTGAAATCATGACCACCCCAGGAAAAGAG
  AACTTTCGCCTGAAAAGTTACAAGAACAAATCTCTGAATCCCGATGAGATGCGCAGGAGG
  AGGGAGGAAGAAGGACTGCAGTTACGAAAGCAGAAAAGAGAAGAGCAGTTATTCAAGCGG
  AGAAATGTTGCTACAGCAGAAGAAGAAACAGAAGAAGAAGTTATGTCAGATGGAGGCTTT
  CATGAGGCTCAGATTAATAACATGGAGATGGCACCAGGTGGTGTCATCACTTCTGACATG
  ATTGAAATGATATTTTCCAAAAGCCCAGAGCAACAGCTTTCAGCAACACAGAAATTCAGG
  AAGCTGCTTTCAAAAGAACCTAACCCTCCTATTGATGAAGTTATCAGCACACCAGGAGTA
  GTGGCCAGGTTTGTGGAGTTCCTCAAACGAAAAGAGAATTGTACACTGCAGTTTGAATCA
  GCTTGGGTACTGACAAATATTGCTTCAGGAAATTCTCTTCAGACCCGAATTGTGATTCAG
  GCAGGAGCTGTGCCCATCTTCATAGAGTTGCTCAGCTCAGAGTTTGAAGATGTCCAGGAA
  CAGGCAGTCTGGGCTCTTGGCAACATTGCTGGAGATAGTACCATGTACAGGGACTATGTC
  TTAGACTGCAATATCCTTCCCCCTCTTTTGCAGTTATTTTCAAAGCAAAACCGCCTGACC
  ATGACCCGGAATGCAGTATGGGCTTTGTCTAATCTCTGTAGAGGGAAAAGTCCACCTCCA
  GAATTTGCAAAGGTTTCTCCATGTCTGAATGTGCTTTCCTGGTTGCTGTTTGTCAGTGAC
  ACTGATGTACTGGCTGATGCCTGCTGGGCCCTCTCATATCTATCAGATGGACCCAATGAT
  AAAATTCAAGCGGTCATCGATGCGGGAGTATGTAGGAGACTTGTGGAACTGCTGATGCAT
  AATGATTATAAAGTGGTTTCTCCTGCTTTGCGAGCTGTGGGAAACATTGTCACAGGGGAT
  GATATTCAGACACAGGTAATTCTGAATTGCTCAGCTCTGCAGAGTTTATTGCATTTGCTG
  AGTAGCCCAAAGGAATCTATCAAAAAGGAAGCATGTTGGACGATATCTAATATTACAGCT
  GGAAATAGGGCACAGATCCAGACTGTGATAGATGCCAACATTTTCCCAGCCCTCATTAGT
  ATTTTACAAACTGCTGAATTTCGGACAAGAAAAGAAGCAGCTTGGGCCATCACAAATGCA
  ACTTCTGGAGGATCAGCTGAACAGATCAAGTACCTAGTAGAACTGGGTTGTATCAAGCCG
  CTCTGTGATCTCCTCACGGTCATGGACTCTAAGATTGTACAGGTTGCCCTAAATGGCTTG
  GAAAATATCCTGAGGCTTGGAGAACAGGAAGCCAAAAGGAATGGCACTGGCATTAACCCT
  TACTGTGCTTTGATTGAAGAAGCTTATGGTCTGGATAAAATTGAGTTCTTACAGAGTCAT
  GAAAACCAGGAGATCTACCAAAAGGCCTTTGATCTTATTGAGCATTACTTCGGGACCGAA
  GATGAAGACAGCAGCATTGCACCCCAGGTTGACCTTAACCAGCAGCAGTACATCTTCCAA
  CAGTGTGAGGCTCCTATGGAAGGTTTCCAGCTTTGAAGCAATACTCTGCTTTCACGTTCC
  TGTGTCAGACCAGGCTACCCAGTCGAGTCCTCTTGTGGAGCCCACAGTCCTCATGGAGCT
  AACTTCTCAAATGTTTTCCATAATACTGTTTGCGCTCATTTGCTTGCCTTGCGCACCTGC
  TCTCTTACACACATCTGGAAAACCTCCGGCTCTCTGTGGTGGAATACCCTTCTAATAAAA
  GGGTAACCAGAACGGCCCACTCTCTTTTACGGAAAAATCCCTAGGCTTTGGAGATCCGCA
  CTTACATTAGAGTTATGGGAATATACACATATTAATGTGGCTCCCTTTTTCTTGTGGGGG
  AATAAAAGAGGACTCCTCCTCATTCCCTTTAACATGGGGGAAAAAACTGACATTAAAAGA
  TGAGACTAAATCTTTATCTTGAATTTTACACAACTACTTAACGACAAGGGAGATGTTTAG
  ACCTGTTGTGTATACTTCAGAGTACTTTTCATGAGTTCTTCCACAGTGAACCCTTGGATT
  ACCTAGTGGCTTTTTCTAGCCAGATTTGCATTAATCCTTACTGAGATTGGATGGTTTTCT
  TTCCTCTATTGGCGCCATTCTTCAGATATTAAAGTTAAACCATCCACTCCCTCACCTTCA
  GCCTTCAGTGAATGTGCTTTCTAGTTGTCAGGAATGCTGAAGAATTAACACTTTGACTCC
  TAAATGTGATACTGGTTTGTAGATTCCCTTAGAGCAGAAAGGAGAGGGGCACATATTAAT
  TTGTATCGCTTTTGCTTCTCTTTGGTCTTTTGTGTCTTAGAATTTGGAAGTGGTTCATTT
  CTGTTGCTGGTATGAGGATTTCGAATACTTAGTAATCGAAAACCATATCCTGTAATTTAA
  TAAAAAAAACTAAGGAAGAAAAAACCCTCCAATTTTCCCAAATGCAATCAGTGTAACTAG
  GGGCTGTGTTTCTGCATTAAAATAAATGTTTCAGGCTTTGTGGTCCTGATCAAGGTCCTC
  ATTAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002264
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002264.2, NP_002255.2
• RefSeq Size: 6887 bp
• RefSeq ORF: 1617 bp
• Locus ID: 3836
• Cytogenetics: 3q21.1
• Domains: Armadillo_seg, IBB
• Gene Summary: 'The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. This protein interacts with the recombination activating gene 1 (RAG1) protein and is a putative substrate of the RAG1 ubiquitin ligase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]'
  Transcript Variant: This variant (1) lacks an alternate exon compared to variant 2 and represents the protein-coding transcript. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.