TEC (NM_003215) Human Untagged Clone

CAT#: SC128212

TEC (untagged)-Human tec protein tyrosine kinase (TEC)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TEC
• Synonyms: PSCTK4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC128212 sequence for NM_003215 edited (data generated by NextGen Sequencing)
  ATGAATTTTAACACTATTTTGGAGGAGATTCTTATTAAAAGGTCACAGCAGAAAAAGAAG
  ACATCGCCCTTAAACTACAAAGAGAGACTTTTTGTACTTACAAAGTCCATGCTAACCTAC
  TATGAGGGTCGAGCAGAGAAGAAATACAGAAAGGGGTTTATTGATGTTTCAAAAATCAAG
  TGTGTGGAAATAGTGAAGAATGATGATGGTGTCATTCCCTGTCAAAATAAGTATCCATTT
  CAGGTTGTTCATGATGCTAACACACTTTACATTTTTGCACCTAGTCCACAAAGCAGGGAC
  CTGTGGGTGAAGAAGTTAAAAGAAGAAATAAAGAACAACAATAATATTATGATTAAATAT
  CATCCTAAATTCTGGACAGATGGAAGTTATCAGTGTTGTAGACAAACTGAAAAATTAGCA
  CCCGGATGTGAAAAATACAATCTTTTTGAGAGTAGTATAAGAAAAGCACTACCTCCAGCA
  CCAGAAACAAAGAAGCGAAGGCCTCCCCCACCAATTCCACTAGAAGAAGAAGATAATAGT
  GAAGAAATCGTTGTAGCCATGTATGATTTCCAAGCAGCAGAAGGACATGATCTCAGATTA
  GAGAGAGGCCAAGAGTATCTCATTTTAGAAAAGAATGATGTTCATTGGTGGAGAGCAAGA
  GATAAATATGGGAATGAAGGATATATCCCAAGTAATTACGTAACGGGAAAGAAATCAAAC
  AACTTAGATCAATATGAATGGTATTGCAGAAATATGAATAGAAGCAAGGCAGAGCAACTC
  CTCCGCAGTGAAGATAAAGAAGGTGGTTTTATGGTAAGGGATTCCAGTCAACCAGGCTTG
  TACACAGTCTCCCTTTATACCAAGTTTGGAGGNNNNGGTTCATCGGGTTTTAGGCATTAT
  CATATAAAGGAAACAACAACATCTCCAAAGAAGTATTACCTAGCTGAAAAACATGCTTTT
  GGCTCCATTCCTGAGATTATTGAATATCATAAGCACAATGCAGCAGGACTTGTCACCAGG
  CTTCGGTACCCAGTTAGTGTGAAAGGGAAGAATGCACCCACCACTGCAGGATTCAGCTAT
  GAGAAATGGGAGATTAACCCTTCAGAACTGACCTTTATGAGGGAATTGGGAAGTGGACTG
  TTTGGAGTGGTGAGGCTTGGCAAATGGCGAGCCCAGTACAAAGTCGCAATCAAAGCTATT
  CGGGAAGGTGCAATGTGCGAGGAGGACTTTATAGAAGAAGCTAAAGTGATGATGAAACTG
  ACACACCCGAAGTTAGTGCAGCTTTATGGTGTGTGCACCCAGCAGAAACCAATATACATT
  GTTACTGAGTTCATGGAAAGGGGCTGCCTTCTGAATTTCCTCCGACAGAGACAAGGTCAT
  TTCAGTAGAGACGTACTGCTGAGCATGTGTCAGGATGTGTGTGAAGGGATGGAGTATCTG
  GAGAGAAACAGCTTCATCCACAGAGATCTGGCTGCCAGAAATTGTCTAGTAAGTGAGGCG
  GGAGTTGTAAAAGTATCTGATTTTGGAATGGCCAGGTATGTTCTGGATGATCAGTACACA
  AGTTCTTCTGGTGCTAAGTTTCCTGTGAAGTGGTGTCCACCTGAAGTGTTTAATTACAGC
  CGCTTCAGCAGCAAATCAGATGTCTGGTCATTTGGTGTTTTAATGTGGGAAGTATTCACG
  GAAGGCAGAATGCCTTTTGAAAAATACACCAATTATGAAGTGGTAACCATGGTTACTCGA
  GGCCACCGACTCTACCAGCCGAAGTTGGCGTCCAACTATGTGTATGAGGTGATGCTGAGA
  TGTTGGCAGGAGAAACCAGAGGGAAGGCCTTCTTTCGAAGATCTGCTGCGCACAATAGAT
  GAACTAGTTGAATGTGAAGAAACTTTTGGAAGATAA
  
  Clone variation with respect to NM_003215.2
  453 c=>t;873 a=>n;874 g=>n;875 a=>n;876 a=>n
• Restriction Sites: Please inquire
• ACCN: NM_003215
• Insert Size: 2600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003215.1, NP_003206.1
• RefSeq Size: 3650 bp
• RefSeq ORF: 1896 bp
• Locus ID: 7006
• Cytogenetics: 4p12-p11
• Domains: pkinase, SH2, TyrKc, SH3, BTK, PH, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: T cell receptor signaling pathway
• Gene Summary: 'The protein encoded by this gene belongs to the Tec family of non-receptor protein-tyrosine kinases containing a pleckstrin homology domain. Tec family kinases are involved in the intracellular signaling mechanisms of cytokine receptors, lymphocyte surface antigens, heterotrimeric G-protein coupled receptors, and integrin molecules. They are also key players in the regulation of the immune functions. Tec kinase is an integral component of T cell signaling and has a distinct role in T cell activation. This gene may be associated with myelodysplastic syndrome. [provided by RefSeq, Jul 2008]'