TEC (NM_003215) Human Untagged Clone

CAT#: SC323542

TEC (untagged)-Kinase deficient mutant (K398M) of Human tec protein tyrosine kinase (TEC)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TEC
• Synonyms: PSCTK4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003215, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAATTTTAACACTATTTTGGAGGAGATTCTTATTAAAAGGTCACAGCAGAAAAAGAAGACATCGCCCT
  TAAACTACAAAGAGAGACTTTTTGTACTTACAAAGTCCATGCTAACCTACTATGAGGGTCGAGCAGAGAA
  GAAATACAGAAAGGGGTTTATTGATGTTTCAAAAATCAAGTGTGTGGAAATAGTGAAGAATGATGATGGT
  GTCATTCCCTGTCAAAATAAGTATCCATTTCAGGTTGTTCATGATGCTAACACACTTTACATTTTTGCAC
  CTAGTCCACAAAGCAGGGACCTGTGGGTGAAGAAGTTAAAAGAAGAAATAAAGAACAACAATAATATTAT
  GATTAAATATCATCCTAAATTCTGGACAGATGGAAGTTATCAGTGTTGTAGACAAACTGAAAAATTAGCA
  CCCGGATGTGAAAAATACAATCTTTTTGAGAGCAGTATAAGAAAAGCACTACCTCCAGCACCAGAAACAA
  AGAAGCGAAGGCCTCCCCCACCAATTCCACTAGAAGAAGAAGATAATAGTGAAGAAATCGTTGTAGCCAT
  GTATGATTTCCAAGCAGCAGAAGGACATGATCTCAGATTAGAGAGAGGCCAAGAGTATCTCATTTTAGAA
  AAGAATGATGTTCATTGGTGGAGAGCAAGAGATAAATATGGGAATGAAGGATATATCCCAAGTAATTACG
  TAACGGGAAAGAAATCAAACAACTTAGATCAATATGAATGGTATTGCAGAAATATGAATAGAAGCAAGGC
  AGAGCAACTCCTCCGCAGTGAAGATAAAGAAGGTGGTTTTATGGTAAGGGATTCCAGTCAACCAGGCTTG
  TACACAGTCTCCCTTTATACCAAGTTTGGAGGAGAAGGTTCATCGGGTTTTAGGCATTATCATATAAAGG
  AAACAACAACATCTCCAAAGAAGTATTACCTAGCTGAAAAACATGCTTTTGGCTCCATTCCTGAGATTAT
  TGAATATCATAAGCACAATGCAGCAGGACTTGTCACCAGGCTTCGGTACCCAGTTAGTGTGAAAGGGAAG
  AATGCACCCACCACTGCAGGATTCAGCTATGAGAAATGGGAGATTAACCCTTCAGAACTGACCTTTATGA
  GGGAATTGGGAAGTGGACTGTTTGGAGTGGTGAGGCTTGGCAAATGGCGAGCCCAGTACAAAGTCGCAAT
  CAAAGCTATTCGGGAAGGTGCAATGTGCGAGGAGGACTTTATAGAAGAAGCTAAAGTGATGATGAAACTG
  ACACACCCGAAGTTAGTGCAGCTTTATGGTGTGTGCACCCAGCAGAAACCAATATACATTGTTACTGAGT
  TCATGGAAAGGGGCTGCCTTCTGAATTTCCTCCGACAGAGACAAGGTCATTTCAGTAGAGACGTACTGCT
  GAGCATGTGTCAGGATGTGTGTGAAGGGATGGAGTATCTGGAGAGAAACAGCTTCATCCACAGAGATCTG
  GCTGCCAGAAATTGTCTAGTAAGTGAGGCGGGAGTTGTAAAAGTATCTGATTTTGGAATGGCCAGGTATG
  TTCTGGATGATCAGTACACAAGTTCTTCTGGTGCTAAGTTTCCTGTGAAGTGGTGTCCACCTGAAGTGTT
  TAATTACAGCCGCTTCAGCAGCAAATCAGATGTCTGGTCATTTGGTGTTTTAATGTGGGAAGTATTCACG
  GAAGGCAGAATGCCTTTTGAAAAATACACCAATTATGAAGTGGTAACCATGGTTACTCGAGGCCACCGAC
  TCTACCAGCCGAAGTTGGCGTCCAACTATGTGTATGAGGTGATGCTGAGATGTTGGCAGGAGAAACCAGA
  GGGAAGGCCTTCTTTCGAAGATCTGCTGCGCACAATAGATGAACTAGTTGAATGTGAAGAAACTTTTGGA
  AGATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_003215 unedited
  CCGCCGTCTGAGCAATGGGCGGTAGGCGCTGTACGGCTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTG
  AACCGTCAGAATATTGTAATACGACTCACTATAGGGCGGCCGCGAATACGGCACGAGCAGGTCCGCCCAG
  CAGCCGCGCTTCAGCCAGAATACTGGGAATCTTCAGTGGCAGGAGGAGTAATCAGAAGACGGAGATGAAT
  TTTAACACTATTTTGGAGGAGATTCTTATTAAAAGGTCACAGCAGAAAAAGAAGACATCGCCCTTAAACT
  ACAAAGAGAGACTTTTTGTACTTACAAAGTCCATGCTAACCTACTATGAGGGTCGAGCAGAGAAGAAATA
  CAGAAAGGGGTTTATTGATGTTTCAAAAATCAAGTGTGTGGAAATAGTGAAGATGATGATGGGTGTCATT
  CCCTGTCAAAATAAGTATCCATTTCAGGTTTGTTCATGATGCTAACACACTTTACATTTTTGCACCTAGT
  CCAACAAAGCAGGGACTGGTGGGTTGAAGAAGTTAAAGAAGAATTAAGGACCACATTATTTATGATTAAT
  TCCTCCTAAATTCTGGAACGAAGGGGATTACGTGGTGAAACAACTGAAAATAGCCCGGAGTGAAAATCCA
  TCTTTTAGAGATGTAAGAAAGCCACACTCCCCCGACCAAACGAAAGCTCCCCCATTCCTAGAGAGATATT
  TGAAATCTGTGCCCGTGTAATTCCACCAAGACGTCTCGATAAGAAGCCAAGTTCTTTAAGAGGTGTCTTG
  TGGACCATATTGGATAGAGATCCGTTCTCCGAATCACCTTTCTATGTGCCATTAGAGCCGGACCTCCCTG
  AATAAGTTGAGGAGTCACTGAATC
  
  Kinase domain sequence

  >SC323542 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TMGACTTTATGAGGGATTGGGAGTGGACTGTTTGGAGTGGTGAGGCTTGGCAAATGGCGAGCCCAGTACA
  AAGTCGCAATCATGGCTATTCGGGAAGGTGCAATGTGCGAGGAGGACTTTATAGAAGAAGCTAAAGTGAT
  GATGAAACTGACACACCCGAAGTTAGTGCAGCTTTATGGTGTGTGCACCCAGCAGAAACCAATATACATT
  GTTACTGAGTTCATGGAAAGGGGCTGCCTTCTGAATTTCCTCCGA
  
• Restriction Sites: Please inquire
• ACCN: NM_003215
• Insert Size: 2600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003215.1, NP_003206.1
• RefSeq Size: 3620 bp
• RefSeq ORF: 1896 bp
• Locus ID: 7006
• Cytogenetics: 4p12-p11
• Domains: pkinase, SH2, TyrKc, SH3, BTK, PH, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: T cell receptor signaling pathway
• Gene Summary: 'The protein encoded by this gene belongs to the Tec family of non-receptor protein-tyrosine kinases containing a pleckstrin homology domain. Tec family kinases are involved in the intracellular signaling mechanisms of cytokine receptors, lymphocyte surface antigens, heterotrimeric G-protein coupled receptors, and integrin molecules. They are also key players in the regulation of the immune functions. Tec kinase is an integral component of T cell signaling and has a distinct role in T cell activation. This gene may be associated with myelodysplastic syndrome. [provided by RefSeq, Jul 2008]'