TEC (NM_003215) Human Untagged Clone

CAT#: SC323542

TEC (untagged)-Kinase deficient mutant (K398M) of Human tec protein tyrosine kinase (TEC)


  "NM_003215" in other vectors (7)

Reconstitution Protocol

USD 760.00

In Stock*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol TEC
Synonyms PSCTK4
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_003215, the custom clone sequence may differ by one or more nucleotides


ATGAATTTTAACACTATTTTGGAGGAGATTCTTATTAAAAGGTCACAGCAGAAAAAGAAGACATCGCCCT
TAAACTACAAAGAGAGACTTTTTGTACTTACAAAGTCCATGCTAACCTACTATGAGGGTCGAGCAGAGAA
GAAATACAGAAAGGGGTTTATTGATGTTTCAAAAATCAAGTGTGTGGAAATAGTGAAGAATGATGATGGT
GTCATTCCCTGTCAAAATAAGTATCCATTTCAGGTTGTTCATGATGCTAACACACTTTACATTTTTGCAC
CTAGTCCACAAAGCAGGGACCTGTGGGTGAAGAAGTTAAAAGAAGAAATAAAGAACAACAATAATATTAT
GATTAAATATCATCCTAAATTCTGGACAGATGGAAGTTATCAGTGTTGTAGACAAACTGAAAAATTAGCA
CCCGGATGTGAAAAATACAATCTTTTTGAGAGCAGTATAAGAAAAGCACTACCTCCAGCACCAGAAACAA
AGAAGCGAAGGCCTCCCCCACCAATTCCACTAGAAGAAGAAGATAATAGTGAAGAAATCGTTGTAGCCAT
GTATGATTTCCAAGCAGCAGAAGGACATGATCTCAGATTAGAGAGAGGCCAAGAGTATCTCATTTTAGAA
AAGAATGATGTTCATTGGTGGAGAGCAAGAGATAAATATGGGAATGAAGGATATATCCCAAGTAATTACG
TAACGGGAAAGAAATCAAACAACTTAGATCAATATGAATGGTATTGCAGAAATATGAATAGAAGCAAGGC
AGAGCAACTCCTCCGCAGTGAAGATAAAGAAGGTGGTTTTATGGTAAGGGATTCCAGTCAACCAGGCTTG
TACACAGTCTCCCTTTATACCAAGTTTGGAGGAGAAGGTTCATCGGGTTTTAGGCATTATCATATAAAGG
AAACAACAACATCTCCAAAGAAGTATTACCTAGCTGAAAAACATGCTTTTGGCTCCATTCCTGAGATTAT
TGAATATCATAAGCACAATGCAGCAGGACTTGTCACCAGGCTTCGGTACCCAGTTAGTGTGAAAGGGAAG
AATGCACCCACCACTGCAGGATTCAGCTATGAGAAATGGGAGATTAACCCTTCAGAACTGACCTTTATGA
GGGAATTGGGAAGTGGACTGTTTGGAGTGGTGAGGCTTGGCAAATGGCGAGCCCAGTACAAAGTCGCAAT
CAAAGCTATTCGGGAAGGTGCAATGTGCGAGGAGGACTTTATAGAAGAAGCTAAAGTGATGATGAAACTG
ACACACCCGAAGTTAGTGCAGCTTTATGGTGTGTGCACCCAGCAGAAACCAATATACATTGTTACTGAGT
TCATGGAAAGGGGCTGCCTTCTGAATTTCCTCCGACAGAGACAAGGTCATTTCAGTAGAGACGTACTGCT
GAGCATGTGTCAGGATGTGTGTGAAGGGATGGAGTATCTGGAGAGAAACAGCTTCATCCACAGAGATCTG
GCTGCCAGAAATTGTCTAGTAAGTGAGGCGGGAGTTGTAAAAGTATCTGATTTTGGAATGGCCAGGTATG
TTCTGGATGATCAGTACACAAGTTCTTCTGGTGCTAAGTTTCCTGTGAAGTGGTGTCCACCTGAAGTGTT
TAATTACAGCCGCTTCAGCAGCAAATCAGATGTCTGGTCATTTGGTGTTTTAATGTGGGAAGTATTCACG
GAAGGCAGAATGCCTTTTGAAAAATACACCAATTATGAAGTGGTAACCATGGTTACTCGAGGCCACCGAC
TCTACCAGCCGAAGTTGGCGTCCAACTATGTGTATGAGGTGATGCTGAGATGTTGGCAGGAGAAACCAGA
GGGAAGGCCTTCTTTCGAAGATCTGCTGCGCACAATAGATGAACTAGTTGAATGTGAAGAAACTTTTGGA
AGATAA


>OriGene 5' read for mutant NM_003215 unedited
CCGCCGTCTGAGCAATGGGCGGTAGGCGCTGTACGGCTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTG
AACCGTCAGAATATTGTAATACGACTCACTATAGGGCGGCCGCGAATACGGCACGAGCAGGTCCGCCCAG
CAGCCGCGCTTCAGCCAGAATACTGGGAATCTTCAGTGGCAGGAGGAGTAATCAGAAGACGGAGATGAAT
TTTAACACTATTTTGGAGGAGATTCTTATTAAAAGGTCACAGCAGAAAAAGAAGACATCGCCCTTAAACT
ACAAAGAGAGACTTTTTGTACTTACAAAGTCCATGCTAACCTACTATGAGGGTCGAGCAGAGAAGAAATA
CAGAAAGGGGTTTATTGATGTTTCAAAAATCAAGTGTGTGGAAATAGTGAAGATGATGATGGGTGTCATT
CCCTGTCAAAATAAGTATCCATTTCAGGTTTGTTCATGATGCTAACACACTTTACATTTTTGCACCTAGT
CCAACAAAGCAGGGACTGGTGGGTTGAAGAAGTTAAAGAAGAATTAAGGACCACATTATTTATGATTAAT
TCCTCCTAAATTCTGGAACGAAGGGGATTACGTGGTGAAACAACTGAAAATAGCCCGGAGTGAAAATCCA
TCTTTTAGAGATGTAAGAAAGCCACACTCCCCCGACCAAACGAAAGCTCCCCCATTCCTAGAGAGATATT
TGAAATCTGTGCCCGTGTAATTCCACCAAGACGTCTCGATAAGAAGCCAAGTTCTTTAAGAGGTGTCTTG
TGGACCATATTGGATAGAGATCCGTTCTCCGAATCACCTTTCTATGTGCCATTAGAGCCGGACCTCCCTG
AATAAGTTGAGGAGTCACTGAATC
>SC323542 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TMGACTTTATGAGGGATTGGGAGTGGACTGTTTGGAGTGGTGAGGCTTGGCAAATGGCGAGCCCAGTACA
AAGTCGCAATCATGGCTATTCGGGAAGGTGCAATGTGCGAGGAGGACTTTATAGAAGAAGCTAAAGTGAT
GATGAAACTGACACACCCGAAGTTAGTGCAGCTTTATGGTGTGTGCACCCAGCAGAAACCAATATACATT
GTTACTGAGTTCATGGAAAGGGGCTGCCTTCTGAATTTCCTCCGA
Restriction Sites Please inquire     
ACCN NM_003215
Insert Size 2600 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003215.1, NP_003206.1
RefSeq Size 3620 bp
RefSeq ORF 1896 bp
Locus ID 7006
Cytogenetics 4p12-p11
Domains pkinase, SH2, TyrKc, SH3, BTK, PH, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways T cell receptor signaling pathway
Gene Summary 'The protein encoded by this gene belongs to the Tec family of non-receptor protein-tyrosine kinases containing a pleckstrin homology domain. Tec family kinases are involved in the intracellular signaling mechanisms of cytokine receptors, lymphocyte surface antigens, heterotrimeric G-protein coupled receptors, and integrin molecules. They are also key players in the regulation of the immune functions. Tec kinase is an integral component of T cell signaling and has a distinct role in T cell activation. This gene may be associated with myelodysplastic syndrome. [provided by RefSeq, Jul 2008]'

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